Current Search: Jia, Pingping (x)
-
-
Title
-
Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells.
-
Creator
-
Jia, Pingping, Zhang, Chi, Jia, Yuanyuan, Webster, Keith A., Huang, Xupei, Kochegarov, Andrei A., Lemanski, Sharon L., Lemanski, Larry F.
-
Date Issued
-
2011-06-22
-
PURL
-
http://purl.flvc.org/fcla/dt/3327268
-
Subject Headings
-
Cell nucleus -- metabolism, Cloning, Molecular, Cytosol --metabolism, Embryonic Stem Cells --metabolism, Methionine --metabolism, Methionine Sulfoxide Reductases, Methionine Sulfoxide Reductases --metabolism, Methionine Sulfoxide Reductases --genetics, Mitochondria --metabolism, Molecular Sequence Data, Oxidation --Reduction, Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Methionine
-
Format
-
Document (PDF)
-
-
Title
-
Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts.
-
Creator
-
Zhang, Chi, Jia, Pingping, Huang, Xupei, Sferrazza, Gian Franco, Athauda, Gagani, Achary, Mohan P., Wang, Jikui, Lemanski, Sharon L., Lemanski, Larry F.
-
Date Issued
-
2009-09-03
-
PURL
-
http://purl.flvc.org/fcla/dt/3327272
-
Subject Headings
-
Ambystoma mexicanum --embryology, Ambystoma mexicanum --genetics, Base Sequence, DNA, Complementary --genetics, Endoderm --physiology, Exons --genetics, Gene Expression Regulation, Developmental, Gene Knockdown Techniques, Genes, Recessive, Heart --embryology, Models, Genetic, Molecular Sequence Data, Muscle Proteins --biosynthesis, Muscle Proteins --genetics, Myocardial Contraction --physiology, Protein Isoforms, Reverse Transcriptase Polymerase Chain Reaction, Tropomyosin
-
Format
-
Document (PDF)
-
-
Title
-
Posttranscriptional regulation of tropomyosin expression by myofibril inducing RNA (MIR) during axolotl embryonic heart development.
-
Creator
-
Jia, Pingping, Florida Atlantic University, Lemanski, Larry F., Charles E. Schmidt College of Medicine, Department of Biomedical Science
-
Abstract/Description
-
A naturally-occurring recessive lethal mutation in axolotls, Ambystoma mexicanum, is an intriguing model for studying tropomyosin expression regulation. Homozygous embryos(c/c) form hearts that are deficient in tropomyosin, lack organized myofibrils and fail to beat. Previous studies have shown that a non-coding RNA gene, MIR (Myofibril Inducing RNA), is sufficient to rescue the non-beating homozygous recessive mutant hearts by promoting sarcomeric tropomyosin expression that leads to...
Show moreA naturally-occurring recessive lethal mutation in axolotls, Ambystoma mexicanum, is an intriguing model for studying tropomyosin expression regulation. Homozygous embryos(c/c) form hearts that are deficient in tropomyosin, lack organized myofibrils and fail to beat. Previous studies have shown that a non-coding RNA gene, MIR (Myofibril Inducing RNA), is sufficient to rescue the non-beating homozygous recessive mutant hearts by promoting sarcomeric tropomyosin expression that leads to formation of organized myofibrils and beating hearts. Real time RT-PCR reveals that mutant hearts express the same level mRNA of the alpha-tropomyosin and TM4 type tropomyosin (ATmC-3) gene as normal embryonic hearts. These genes show no differences with regard to the splicing patterns of normal and mutant. Using protease inhibitor LLnL and E-64d treatments and two-dimensional Western blots of normal and mutant hearts, it is found that mutant hearts express all tropomyosin protein isoforms as normal hearts but protein expression are at low levels. These studies suggest that there is a failure in the translational or posttranslational control mechanisms for tropomyosin protein synthesis in cardiac mutant axolotl hearts during development.
Show less
-
Date Issued
-
2006
-
PURL
-
http://purl.flvc.org/fcla/dt/13380
-
Subject Headings
-
Medical genetics, Molecular biology, Cell differentiation, Gene expression, Axolotls--Development, Heart--Growth--Molecular aspects
-
Format
-
Document (PDF)
