Current Search: Jerez, Diego Alejandro (x)
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Title
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DEVELOPING A DEEP LEARNING PIPELINE TO AUTOMATICALLY ANNOTATE GOLD PARTICLES IN IMMUNOELECTRON MICROSCOPY IMAGES.
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Creator
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Jerez, Diego Alejandro, Hahn, William, Florida Atlantic University, Department of Mathematical Sciences, Charles E. Schmidt College of Science
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Abstract/Description
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Machine learning has been utilized in bio-imaging in recent years, however as it is relatively new and evolving, some researchers who wish to utilize machine learning tools have limited access because of a lack of programming knowledge. In electron microscopy (EM), immunogold labeling is commonly used to identify the target proteins, however the manual annotation of the gold particles in the images is a time-consuming and laborious process. Conventional image processing tools could provide...
Show moreMachine learning has been utilized in bio-imaging in recent years, however as it is relatively new and evolving, some researchers who wish to utilize machine learning tools have limited access because of a lack of programming knowledge. In electron microscopy (EM), immunogold labeling is commonly used to identify the target proteins, however the manual annotation of the gold particles in the images is a time-consuming and laborious process. Conventional image processing tools could provide semi-automated annotation, but those require that users make manual adjustments for every step of the analysis. To create a new high-throughput image analysis tool for immuno-EM, I developed a deep learning pipeline that was designed to deliver a completely automated annotation of immunogold particles in EM images. The program was made accessible for users without prior programming experience and was also expanded to be used on different types of immuno-EM images.
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Date Issued
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2020
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PURL
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http://purl.flvc.org/fau/fd/FA00013628
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Subject Headings
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Electron microscopy, Immunogold labeling, Image analysis, Deep learning
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Format
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Document (PDF)
