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- Title
- DEVELOPMENT OF A SENSITIVE POLYMERASE CHAIN REACTION (PCR) APPROACH TO DETECT ENTAMOEBA DISPAR IN INDUSTRIAL WASTEWATER.
- Creator
- Chowdhury, Rakib Ahmed, Meeroff, Daniel E., Florida Atlantic University, Department of Civil, Environmental and Geomatics Engineering, College of Engineering and Computer Science
- Abstract/Description
-
Deep injection wells are considered among the most efficient, environmentally-friendly and cost-effective techniques to dispose of wastewater. However, formation of biofilms in the casing pipe can reduce the effective diameter, which in turn, can lower the injectivity of wastewater and ultimately results in injection failure. A class 1 deep injection well located at the Solid Waste Authority of Palm Beach County was revealed to be getting clogged due to the development of a microbial...
Show moreDeep injection wells are considered among the most efficient, environmentally-friendly and cost-effective techniques to dispose of wastewater. However, formation of biofilms in the casing pipe can reduce the effective diameter, which in turn, can lower the injectivity of wastewater and ultimately results in injection failure. A class 1 deep injection well located at the Solid Waste Authority of Palm Beach County was revealed to be getting clogged due to the development of a microbial community where Entamoeba dispar, a protozoan species was found to be the abundant microorganism in the biofilm. The injection well is used to discharge industrial wastewater coming from several sources at the facility which are discharged to a collection chamber, known as the wet well, before being disposed down the deep injection well pipe. Prior to design and implementation of a suitable treatment technique to inactivate the protozoan species, it is imperative to reveal the origins of the microorganism coming to the deep injection well. Therefore, the objective of the current research was to develop a technique to identify potential sources of Entamoeba dispar. In this study, samples were collected from the seven sources as well as from the wet well. Initially, a number of onsite and laboratory experiments were conducted to monitor the water quality parameters of the collected samples. In case of microbiological investigations, microscopic analysis was carried out to detect the microorganism in the wastewater specimens followed by polymerase chain reaction (PCR) and gel-electrophoresis assays. In addition, the number of DNA copies in each of the tested samples was determined using the ImageJ app. From the microscopic analysis, no samples were found to be Entamoeba dispar positive. However, PCR and gel electrophoresis tests results indicated that wet well, NEFCO effluent, class 1, REF 1 and groundwater dilution samples were positive and the calculated number of DNA copies were 6545, 6849, 16763, 6351 and 5635 in 100 mL of the wastewater specimens respectively. The PCR technique used in this study is sensitive enough to detect even 4 DNA copies of the target microorganism. All the positive samples have one thing in common, which is they all contain local groundwater from site, indicating a potential source for further investigation.
Show less - Date Issued
- 2021
- PURL
- http://purl.flvc.org/fau/fd/FA00013871
- Subject Headings
- Entamoeba, Industrial wastewater, Sewage, Polymerase chain reaction
- Format
- Document (PDF)
- Title
- AN EXPERIMENTAL APPROACH TO EVALUATE THE EFFECTIVENESS AND PERFORMANCE OF CHLORINE AS A DISINFECTANT FOR ENTAMOEBA DISPAR IN GROUNDWATER.
- Creator
- Chowdhury. Rakib Ahmed, Lashaki, Masoud Jahandar, Meeroff, Daniel E., Florida Atlantic University, Department of Civil, Environmental and Geomatics Engineering, College of Engineering and Computer Science
- Abstract/Description
-
Deep injection well technology is a reliable and cost-effective technique to manage hazardous wastewater. However, reduced injectivity is an issue for the performance of an injection well which can happen due to the occurrence of biogeochemical clogging. A class 1 deep injection well located at the Solid Waste Authority of Palm Beach County has long suffered similar problems that occurred due to the formation of chemical precipitation and biofilm. In the case of the biofilm, the dominant...
Show moreDeep injection well technology is a reliable and cost-effective technique to manage hazardous wastewater. However, reduced injectivity is an issue for the performance of an injection well which can happen due to the occurrence of biogeochemical clogging. A class 1 deep injection well located at the Solid Waste Authority of Palm Beach County has long suffered similar problems that occurred due to the formation of chemical precipitation and biofilm. In the case of the biofilm, the dominant microorganism detected in previous work was determined to be Entamoeba dispar. The prime source of the protozoan was identified as the local groundwater, which is employed for different purposes within the solid waste facility, such as cooling water and dilution water. Therefore, it is imperative to examine the effectiveness of the commonly used disinfectant chlorine to inactivate the protozoan to eliminate biofilms and clogging. This study conducted a laboratory-based chlorination of the groundwater sample to reveal the required dosages of chlorine needed for 3.0-log inactivation of E. dispar in various temperature (20°C, 25°C, 30°C, and 35°C) and pH (6.5, 7.0, 7.5) conditions.
Show less - Date Issued
- 2024
- PURL
- http://purl.flvc.org/fau/fd/FA00014439
- Subject Headings
- Groundwater, Entamoeba dispar, Chlorine, Injection wells
- Format
- Document (PDF)