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Biological electron transfer
Title
Biological electron transfer: an investigation of riboflavin.
Creator
Caplan, Stacee Lee, Harriet L. Wilkes Honors College
Abstract/Description
Most living organisms utilize electron transport chains in order to obtain energy. Riboflavin, commonly known as vitamin B2, is the central component of the redox coenzymes flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN). These cofactors serve as a prosthetic group to flavoproteins and function as the energy-carrying molecules in electron transfer reactions. In this study, the different ionization and oxidation states of riboflavin were identified and quantified as a...
Show moreMost living organisms utilize electron transport chains in order to obtain energy. Riboflavin, commonly known as vitamin B2, is the central component of the redox coenzymes flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN). These cofactors serve as a prosthetic group to flavoproteins and function as the energy-carrying molecules in electron transfer reactions. In this study, the different ionization and oxidation states of riboflavin were identified and quantified as a function of solution potential and pH. To accomplish this task, spectroelectrochemical reductions of riboflavin at different pH were performed. Spectroscopic data offer clues concerning the identity of underlying species, such as oxidation/ionization states and the controlling equilibria. The large data sets obtained from these experiments were analyzed and the acid dissociation constant for reduced riboflavin was determined.
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Date Issued
2008
PURL
http://purl.flvc.org/FAU/77660
Subject Headings
Solution (Chemistry), Electron spectroscopy, Chemical reaction, Conditions and laws of, Charge transfer in biology
Format
Document (PDF)
Rapid neuroprotection from acute oxidative stress
Title
Rapid neuroprotection from acute oxidative stress.
Creator
Caplan, Stacee Lee, Dawson-Scully, Ken, Milton, Sarah L., Graduate College
Date Issued
2011-04-08
PURL
http://purl.flvc.org/fcla/dt/3164512
Subject Headings
Stress --physiology, Protein kinases, Neuroprotective agents
Format
Document (PDF)
A cGMP-dependent protein kinase (PKG) controls synaptic transmission tolerance to acute oxidative stress at the Drosophila larval neuromuscular junction
Title
A cGMP-dependent protein kinase (PKG) controls synaptic transmission tolerance to acute oxidative stress at the Drosophila larval neuromuscular junction.
Creator
Caplan, Stacee Lee, Milton, Sarah L., Dawson-Scully, Ken
Date Issued
2013-02-01
PURL
http://purl.flvc.org/fau/flvc_fau_islandoraimporter_10.1152_jn.00784.2011_1644861569
Format
Document (PDF)
Pseudopterosin A: Protection of Synaptic Function and Potential as a Neuromodulatory Agent
Title
Pseudopterosin A: Protection of Synaptic Function and Potential as a Neuromodulatory Agent.
Creator
Caplan, Stacee Lee, Zheng, Bo, Dawson-Scully, Ken, White, Catherine, West, Lyndon
Date Issued
2016-03-10
PURL
http://purl.flvc.org/fau/fd/FAUIR000130
Format
Citation
Neuroprotection During Acute Oxidative Stress: Role of the PKG Pathway and Identification of Novel Neuromodulatory Agents Using Drosophila Melanogaster
Title
Neuroprotection During Acute Oxidative Stress: Role of the PKG Pathway and Identification of Novel Neuromodulatory Agents Using Drosophila Melanogaster.
Creator
Caplan, Stacee Lee, Dawson-Scully, Ken, Milton, Sarah L., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description
Oxidant stress and injury is inherent in many human diseases such as ischemic vascular and respiratory diseases, heart failure, myocardial infarction, stroke, perinatal and placental insufficiencies, diabetes, cancer, and numerous psychiatric and neurodegenerative disorders. Finding novel therapeutics to combat the deleterious effects of oxidative stress is critical to create better therapeutic strategies for many conditions that have few treatment options. This study used the anoxia-tolerant...
Show moreOxidant stress and injury is inherent in many human diseases such as ischemic vascular and respiratory diseases, heart failure, myocardial infarction, stroke, perinatal and placental insufficiencies, diabetes, cancer, and numerous psychiatric and neurodegenerative disorders. Finding novel therapeutics to combat the deleterious effects of oxidative stress is critical to create better therapeutic strategies for many conditions that have few treatment options. This study used the anoxia-tolerant fruit fly, Drosophila melanogaster, to investigate endogenous cellular protection mechanisms and potential interactions to determine their ability to regulate synaptic functional tolerance and cell survival during acute oxidative stress. The Drosophila larval neuromuscular junction (NMJ) was used to analyze synaptic transmission and specific motor axon contributions. Drosophila Schneider 2 (S2) cells were used to assess viability. Acute oxidative stress was induced using p harmacological paradigms that generate physiologically relevant oxidant species: mitochondrial superoxide production induced by sodium azide (NaN3) and hydroxyl radical formation via hydrogen peroxide (H2O2). A combination of genetic and pharmacological approaches were used to explore the hypothesis that endogenous protection mechanisms control cellular responses to stress by manipulating ion channel conductance and neurotransmission. Furthermore, this study analyzed a group of marine natural products, pseudopterosins, to identify compounds capable of modulating synaptic transmission during acute oxidative stress and potential novel neuromodulatory agents.
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Date Issued
2015
PURL
http://purl.flvc.org/fau/fd/FA00004487, http://purl.flvc.org/fau/fd/FA00004487
Subject Headings
Drosophila melanogaster -- Life cycles, Oxidative stress -- Ecophysiology, Oxidative stress -- Prevention, Protein kinases, Proteins -- Chemical modification
Format
Document (PDF)