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The NSAID, Sulindac confers protection against oxidative stress induced damage in retinal pigmented epithelial cells
Title
The NSAID, Sulindac confers protection against oxidative stress induced damage in retinal pigmented epithelial cells.
Creator
Sur, Arunodoy, Biswal, Manas, Kreymerman, Alexander, Weissbach, Herbert, Prentice, Howard, Blanks, Janet C., Graduate College
Date Issued
2011-04-08
PURL
http://purl.flvc.org/fcla/dt/3164798
Subject Headings
Nonsteroidal anti-inflammatory agents, Oxidative stress, Epithelial cells
Format
Document (PDF)
Hypoxia-regulated glial cell-specific gene therapy to treat retinal neovascularization
Title
Hypoxia-regulated glial cell-specific gene therapy to treat retinal neovascularization.
Creator
Biswal, Manas Ranjan., Charles E. Schmidt College of Science, Center for Complex Systems and Brain Sciences
Abstract/Description
Diabetic retinopathy is an ischemic retinal neovascular disease causing vision loss among adults. The studies presented involve the design and testing of a gene therapy vector to inhibit retinal revascularization, similar to that found in diabetic retinopathy. Gene therapy has proven to be an effective method to introduce therapeutic proteins to treat retinal diseases. Targeting a specific cell type and expression of therapeutic proteins according to the tissue microenvironment should have an...
Show moreDiabetic retinopathy is an ischemic retinal neovascular disease causing vision loss among adults. The studies presented involve the design and testing of a gene therapy vector to inhibit retinal revascularization, similar to that found in diabetic retinopathy. Gene therapy has proven to be an effective method to introduce therapeutic proteins to treat retinal diseases. Targeting a specific cell type and expression of therapeutic proteins according to the tissue microenvironment should have an advantage over traditional gene therapy by avoiding unwanted transgene expression. Hypoxia plays a significant role in the pathophysiology of many retinal ischemic diseases. Retinal Mèuller cells provide structural and functional support to retinal neurons, as well as playing a significant role in retinal neovascularization. Targeting Mèuller cells may be an effective strategy to prevent retinal neovascularization under pathological conditions. ... The hypoxia regulated, glial specific vector successfully reduced the abnormal neovascularization in the periphery by 93% and reduced the central vasobliterated area by 90%. A substantial amount of exogenous endostatin was produced in the retinas of P17 OIR mice. A significant increase in human endostatin protein and reduced vascular endothelial growth factor (VEGF) were identified by Western blot and ELISA, respectively. These findings suggest hypoxia-regulated, glial cell-specific scAAV mediated gene expression may be useful to prevent blindness found in devastating retinal diseases involving neovascularization.
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Date Issued
2012
PURL
http://purl.flvc.org/FAU/3359290
Subject Headings
Diabetic retinopathy, Research, Methodology, Gene therapy, Retinal degeneration, Treatment, Neovascularization inhibitors, Mitochondrial pathology, Retina, Cytology, Gene mapping
Format
Document (PDF)