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Title: Effects of MsrA and MsrB During Anoxic Stress in Drosophila melanogaster.
Creator: Suthakaran, Nirthieca, Binninger, David
Abstract/Description: Drosophila melanogaster can withstand hours of oxygen deprivation (anoxia) by entering a protective coma called spreading depression. When oxygen is reintroduced to the cells, a burst of reactive oxygen species (ROS) causes oxidative damage. Methionine is susceptible to oxidation to form methionine sulfoxide. This oxidation is reversible where methionine sulfoxide reductase (Msr) A and B reduce the S and R enantiomers, respectively. In this study, MsrA and MsrB single deletion lines were...
Show moreDrosophila melanogaster can withstand hours of oxygen deprivation (anoxia) by entering a protective coma called spreading depression. When oxygen is reintroduced to the cells, a burst of reactive oxygen species (ROS) causes oxidative damage. Methionine is susceptible to oxidation to form methionine sulfoxide. This oxidation is reversible where methionine sulfoxide reductase (Msr) A and B reduce the S and R enantiomers, respectively. In this study, MsrA and MsrB single deletion lines were exposed to one hour of anoxia and the Drosophila Activity Monitor (DAM) recorded their recovery times. RNA interference (RNAi) lines were used to mimic the effect of these deletion lines by ubiquitously knocking down their expression. My current data indicates that MsrA loss-of-function strains recover significantly faster than the MsrB loss-of-function lines with increasing age. Insight into the roles of Msr genes under anoxic stress could lead to a better understanding of how these genes contribute to aging.
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Date Issued: 2014
PURL: http://purl.flvc.org/fau/fd/FA0005037
Subject Headings: College students --Research --United States.
Format: Document (PDF)

Title: Does methionine sulfoxide reductase have a role in maintaining adequate dopamine levels in drosophila melanogaster?.
Creator: Hernandez, Caesar, Binninger, David, Graduate College
Date Issued: 2013-04-12
PURL: http://purl.flvc.org/fcla/dt/3361311
Subject Headings: Drosophila melanogaster, Methionine Sulfoxide Reductases, Dopamine
Format: Document (PDF)

Title: Methionine Sulfoxide Reductase Msr Deficiency Leads to a Reduction in Dopamine Levels in Drosophila.
Creator: Hernandez, Caesar, Weissbach, Herbert, Binninger, David, Graduate College
Abstract/Description: Biological homeostasis relies on protective mechanisms that respond to cellular oxidation caused primarily by free radical reactions. Methionine sulfoxide reductases Msr are a class of enzymes that reverse oxidative damage to methionine. The focus of this study is on the relationship between Msr and dopamine in Drosophila. Dopaminergic neurons in drosophila have comparable roles to those found in humans. A deficit in dopamine leads to the onset of many neurological disorders including the...
Show moreBiological homeostasis relies on protective mechanisms that respond to cellular oxidation caused primarily by free radical reactions. Methionine sulfoxide reductases Msr are a class of enzymes that reverse oxidative damage to methionine. The focus of this study is on the relationship between Msr and dopamine in Drosophila. Dopaminergic neurons in drosophila have comparable roles to those found in humans. A deficit in dopamine leads to the onset of many neurological disorders including the loss of fine motor control—a neurodegenerative condition characteristic of Parkinson’s disease PD. We have found that dopamine levels in the heads of MsrAΔ/ΔBΔ/Δ mutants are significantly reduced in comparison to the wild type. In addition, we have found that TH protein and expression levels are markedly reduced in an Msr-deficient system. Our findings suggest that it is possible the Msr system plays an important role in maintaining dopaminergic neurons alive, and thus, is protectant of the CNS.
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Date Issued: 2014
PURL: http://purl.flvc.org/fau/fd/FA00005820
Format: Document (PDF)

Title: Effects of anoxia on methionine sulfoxide reductase (Msr) deficient drosophila.
Creator: Howard, Danielle, Binninger, David
Date Issued: 2013-04-05
PURL: http://purl.flvc.org/fcla/dt/3361093
Subject Headings: Anoxia, Drosophila, Reactive Oxygen Species
Format: Document (PDF)

Title: The Effects of Methionine Sulfoxide Reductase (Msr) Deficiency During Anoxia in Drosophila melanogaster.
Creator: Victoria Beck, David Binninger
Date Issued: 2017
PURL: http://purl.flvc.org/fau/fd/FAU_SR00000003
Subject Headings: College students --Research --United States.
Format: Document (PDF)

Title: Sulindac Enhances the Killing of Cancer Cells Exposed to Oxidative Stress.
Creator: Marchetti, Maria, Resnick, Lionel, Gamliel, Edna, Kesaraju, Shailaja, Weissbach, Herbert, Binninger, David, Bauer, Joseph Alan
Date Issued: 2009-06-05
PURL: http://purl.flvc.org/fau/fd/FAUIR000108
Format: Citation

Title: The role of methionine sulfoxide reductase in thermal stress response.
Creator: Martin, James, Bruce, Lindsay, Schey, Karin, Binninger, David
Date Issued: 2013-04-05
PURL: http://purl.flvc.org/fcla/dt/3361149
Subject Headings: Hyperthermia, Heat shock proteins, Methionine, Oxidative stress
Format: Document (PDF)

Title: Effects of power line (60 Hz) electromagnetic fields on protein phosphorylation in the yeast Saccharomyces cerevisiae.
Creator: Ross, Mary Alice, Florida Atlantic University, Binninger, David
Abstract/Description: Epidemiological studies have suggested that 60 Hz AC electromagnetic fields (EMF) generated by power lines might play a role in the increased incidence of certain human cancers, especially in children. Previous studies with human cells indicate that one molecular mechanism may involve differential effects on protein phosphorylation. The aim of this thesis was to determine whether 60 HZ EMF also affect phosphorylation in the yeast Saccharomyces cerevisiae which has been developed into a...
Show moreEpidemiological studies have suggested that 60 Hz AC electromagnetic fields (EMF) generated by power lines might play a role in the increased incidence of certain human cancers, especially in children. Previous studies with human cells indicate that one molecular mechanism may involve differential effects on protein phosphorylation. The aim of this thesis was to determine whether 60 HZ EMF also affect phosphorylation in the yeast Saccharomyces cerevisiae which has been developed into a paradigm for molecular biologists. The data presented in this thesis show that protein phosphorylation is indeed altered in yeast in response to 60 Hz EMF. This work validates yeast as an experimental model for more in depth investigation of the molecular and genetic basis of this response. Insight gained from these studies should shed light on the interaction of power line EMF with human cells and its possible role in development of human cancers.
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Date Issued: 1995
PURL: http://purl.flvc.org/fcla/dt/15145
Subject Headings: Electric lines--Health aspects, Electromagnetic fields--Health aspects, Saccharomyces cerevisiae
Format: Document (PDF)

Title: Molecular analysis of two yeast genes whose expression is induced by power line electromagnetic fields.
Creator: Busch, Annette., Florida Atlantic University, Binninger, David
Abstract/Description: It has been suggested that 60 Hz electromagnetic fields (EMF) from power lines might present a health hazard to humans. Toward understanding the molecular mechanisms, two genes--EMF-A and EMF-B--had been tentatively identified in Saccharomyces cerevisiae as being responsive to EMF. The aim of this thesis was to confirm that EMF-A and EMF-B were convincingly and reproducibly inducible by 60 Hz EMF. The results confirmed that expression of both genes was induced following exposure to 60 Hz EMF....
Show moreIt has been suggested that 60 Hz electromagnetic fields (EMF) from power lines might present a health hazard to humans. Toward understanding the molecular mechanisms, two genes--EMF-A and EMF-B--had been tentatively identified in Saccharomyces cerevisiae as being responsive to EMF. The aim of this thesis was to confirm that EMF-A and EMF-B were convincingly and reproducibly inducible by 60 Hz EMF. The results confirmed that expression of both genes was induced following exposure to 60 Hz EMF. DNA sequence analysis of these genes showed a nearly perfect match to two Saccharomyces cerevisiae genes in the GenBank DNA database. The available data indicates that while both genes have been previously identified in other laboratories, precise identification of their biological functions has not been established. The cloned genes will provide valuable molecular probes for the ongoing research into the biological effects of power line EMF.
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Date Issued: 1995
PURL: http://purl.flvc.org/fcla/dt/15116
Subject Headings: Electromagnetic fields--Health aspects, Electric lines--Health aspects, Saccharomyces cerevisiae, Nucleotide sequence
Format: Document (PDF)

Title: Progress towards genetic engineering of an EMF-responsive plasmid in yeast.
Creator: Crenshaw, Karin Ann., Florida Atlantic University, Binninger, David
Abstract/Description: Despite the many epidemiological studies which have shown some effects of EMF on biological systems, there has yet to be any data that indicates the molecular mechanisms by which this effect takes place. My goal was to genetically engineer a yeast strain that would have a clear biological effect to the EMF's. The strategy involved using a yeast strain which requires histone function from a plasmid, and the plasmid construct that has a Gal1 promoter controlling the histone function. The...
Show moreDespite the many epidemiological studies which have shown some effects of EMF on biological systems, there has yet to be any data that indicates the molecular mechanisms by which this effect takes place. My goal was to genetically engineer a yeast strain that would have a clear biological effect to the EMF's. The strategy involved using a yeast strain which requires histone function from a plasmid, and the plasmid construct that has a Gal1 promoter controlling the histone function. The plasmid construct could then be engineered to contain a promoter sequence for a known EMF-repressed gene in yeast, which would control the histone production. Without a functional histone gene, the cells will die, and the effects will be easily visualized. Although the genetic screening for the desired transformants appeared to work, the molecular analysis of those transformants did not show the promoter insertion. There are a few possible reasons for why this happened, including possible reversions from one of the original mutations of the chromosomal histone H4 genes, or the mutation of the Gal1 promoter which would no longer repress the histone H4 gene and allow the cells to grow on glucose.
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Date Issued: 1998
PURL: http://purl.flvc.org/fcla/dt/15572
Subject Headings: Electromagnetism--Physiological effect, Genetic engineering, Plasmids--Genetics
Format: Document (PDF)

Title: Role of Methionine Sulfoxide Reductase (MsrA) on Aging and Oxidative Stress in Drosophila.
Creator: Foss, Katie, Binninger, David, Florida Atlantic University
Abstract/Description: Oxidative damage is an inevitable consequence of aerobic respiration. Methionine sulfoxide reductases (Msr) are a group of enzymes that function to repair oxidized methionine residues in both free methionine and methionine in proteins. MsrA was the first of these enzymes to be discovered and is the most thoroughly studied. It is thought to play a role in both the aging process and probably several neurodegenerative diseases. I recently obtained a strain of Drosophila that was reported to have...
Show moreOxidative damage is an inevitable consequence of aerobic respiration. Methionine sulfoxide reductases (Msr) are a group of enzymes that function to repair oxidized methionine residues in both free methionine and methionine in proteins. MsrA was the first of these enzymes to be discovered and is the most thoroughly studied. It is thought to play a role in both the aging process and probably several neurodegenerative diseases. I recently obtained a strain of Drosophila that was reported to have a P-element transposon located within Exon 2 (part of the open reading frame) of the eip71cd gene, which is the Drosophila homolog of MsrA. Thus, the transposon insertion should disrupt expression of the msrA gene. I did a series of experiments to "jump out" the P-element in an effort to recover two types of isogenic strains. The first would be a null mutation of the MsrA gene created by deletion of flanking genomic DNA when the P-element excised from the chromosome. The second would be a precise excision of the P-element, which would restore the genetic locus to its original structure. This study looks at the effect of a null mutant of the MsrA gene on aging and resistance to oxidative stress.
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Date Issued: 2006
PURL: http://purl.flvc.org/fau/fd/FA00000772
Subject Headings: Genetic regulation, Oxidation-reduction reaction, Antioxidants, Oxygen--Physiological effect, Proteins--Chemical modification
Format: Document (PDF)

Title: Novel DNA sequences of the endangered Florida Semaphore cactus.
Creator: Dougherty, Christopher James., Florida Atlantic University, Binninger, David
Abstract/Description: The population decline in the Florida Semaphore cactus, Opuntia corallicola, due to grazing by the moth, Cactoblastus cactorum, has placed this plant species on the verge of extinction in the Florida Keys. Although this semaphore cactus is endemic to Florida, it was mis-named as an almost uncommon Jamaican species (Opuntia spinosissima) in the 1970s. Our goal was to provide genetic data to distinguish these cacti with nuclear and chloropast genes previously used in other plant phylogenetic...
Show moreThe population decline in the Florida Semaphore cactus, Opuntia corallicola, due to grazing by the moth, Cactoblastus cactorum, has placed this plant species on the verge of extinction in the Florida Keys. Although this semaphore cactus is endemic to Florida, it was mis-named as an almost uncommon Jamaican species (Opuntia spinosissima) in the 1970s. Our goal was to provide genetic data to distinguish these cacti with nuclear and chloropast genes previously used in other plant phylogenetic analyses. Phylogenetic relationships were inferred from nucleotide sequence variation in both the Leucine-Phenylalanine transfer RNA intergenic spacer (trnL-trnF) and ribulose bisphosphate carboxylase-oxygenase (rbcL) genes of cactus chloropast DNA. While the trnL-trnF gene sequence data could only group cacti at the genus level, it was the data from the rbcL gene which show speciation and differences between the Florida Semaphore cactus, Opuntia corallicola, and the Jamaican species, Opuntia spinosissima. Sequence data from the internal transcribed spacer (ITS) regions of nuclear DNA were not available due to the divergence of the cactus ITS region from the universal primers sequences used to initiate the polymerase chain reaction (PCR).
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Date Issued: 1996
PURL: http://purl.flvc.org/fcla/dt/15335
Subject Headings: Cactus--Classification, Plant cytotaxonomy, Opuntia
Format: Document (PDF)

Title: The biosynthetic production of pseudopterosin C: An anti-inflammatory agent from a marine soft coral.
Creator: Mydlarz, Laura D., Florida Atlantic University, Kerr, Russell G., Binninger, David
Abstract/Description: Pseudopterosin C is a novel diterpene glycoside isolated from the Caribbean gorgonian coral, Pseudopterogorgia elisabethae. Pseudopterosin C is non-steroidal and has been shown to exhibit promising anti-inflammatory and analgesic properties. The in vitro production of pseudopterosin C through biosynthetic methods has been examined. A cell-free system as well as an enzyme precipitate capable of transforming terpene precursors to the product has been developed. The parameters toward optimizing...
Show morePseudopterosin C is a novel diterpene glycoside isolated from the Caribbean gorgonian coral, Pseudopterogorgia elisabethae. Pseudopterosin C is non-steroidal and has been shown to exhibit promising anti-inflammatory and analgesic properties. The in vitro production of pseudopterosin C through biosynthetic methods has been examined. A cell-free system as well as an enzyme precipitate capable of transforming terpene precursors to the product has been developed. The parameters toward optimizing the enzymatic production through cofactor requirements, differing terpene precursors and sugars were investigated.
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Date Issued: 1998
PURL: http://purl.flvc.org/fcla/dt/15588
Subject Headings: Anti-inflammatory agents, Alcyonacea, Synthetic drugs
Format: Document (PDF)

Title: In Vivo Effects of Methionine Sulfoxide Reductase Deficiency in Drosophila melanogaster.
Creator: Bruce, Lindsay, Singkornrat, Diana, Wilson, Kelsey, Hausman, William, Robbins, Kelli, Huang, Lingxi, Foss, Katie, Binninger, David
Date Issued: 2018-11-01
PURL: http://purl.flvc.org/fau/flvc_fau_islandoraimporter_10.3390_antiox7110155_1634240527
Format: Citation

Title: Alternative Biological Roles of Methionine Sulfoxide Reductases in Drosophila melanogaster.
Creator: Wilson, Kelsey, Binninger, David, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: The oxidation of methionine (Met) into methionine sulfoxide (met-(o)) leads to deleterious modifications to a variety of cellular constituents. These deleterious alterations can be reversed by enzymes known as methionine sulfoxide reductases (Msr). The Msr (MsrA and MsrB) family of enzymes have been studied extensively for their biological roles in reducing oxidized Met residues back into functional Met. A wide range of studies have focused on Msr both in vivo and in vitro using a variety of...
Show moreThe oxidation of methionine (Met) into methionine sulfoxide (met-(o)) leads to deleterious modifications to a variety of cellular constituents. These deleterious alterations can be reversed by enzymes known as methionine sulfoxide reductases (Msr). The Msr (MsrA and MsrB) family of enzymes have been studied extensively for their biological roles in reducing oxidized Met residues back into functional Met. A wide range of studies have focused on Msr both in vivo and in vitro using a variety of model organisms. More specifically, studies have noted numerous processes affected by the overexpression, under expression, and silencing of MsrA and MsrB. Collectively, the results of these studies have shown that Msr is involved in lifespan and the management of oxidative stress. More recent evidence is emerging that supports existing biological functions of Msr and theorizes the involvement of Msr in numerous biological pathways.
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Date Issued: 2018
PURL: http://purl.flvc.org/fau/fd/FA00005980
Subject Headings: Drosophila melanogaster, Methionine Sulfoxide Reductases, Oxidative stress
Format: Document (PDF)

Title: Developmental delays in methionine sulfoxide reductase mutants in Drosophila Melanogaster.
Creator: Hausman, William, Binninger, David, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Aging is a biological process that has many detrimental effects due to the accumulation of oxidative damage to key biomolecules due to the action of free radicals. Methionine sulfoxide reductase (Msr) functions to repair oxidative damage to methionine residues. Msr comes in two forms, MsrA and MsrB, each form has been shown to reduce a specific enantiomer of bound and free oxidized methionine. Effects of Msr have yet to be studied in the major developmental stages of Drosophila melanogaster...
Show moreAging is a biological process that has many detrimental effects due to the accumulation of oxidative damage to key biomolecules due to the action of free radicals. Methionine sulfoxide reductase (Msr) functions to repair oxidative damage to methionine residues. Msr comes in two forms, MsrA and MsrB, each form has been shown to reduce a specific enantiomer of bound and free oxidized methionine. Effects of Msr have yet to be studied in the major developmental stages of Drosophila melanogaster despite the enzymes elevated expression during these stages. A developmental timeline was determined for MsrA mutant, MsrB mutant, and double null mutants against a wild type control. Results show that the Msr double mutant is delayed approximately 20 hours in the early/mid third instar stage while each of the single mutants showed no significant difference to the wild type. Data suggests that the reasoning of this phenomenon is due to an issue gaining mass.
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Date Issued: 2014
PURL: http://purl.flvc.org/fau/fd/FA00004200, http://purl.flvc.org/fau/fd/FA00004200
Subject Headings: Aging -- Molecular aspects, Cellular signal transduction, Drosophila melanogaster -- Genetics, Mitochondrial pathology, Mutation (Biology), Oxidative stress
Format: Document (PDF)

Title: Effects of Serotonin Modulation on Methionine Sulfoxide Reductase Deficient Drosophila melanogaster.
Creator: Hamadeh, Ali, Binninger, David, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Methionine sulfoxide reductase (MSR) is an important antioxidant to help mitigate oxidative stress that contributes to age-associated neurodegenerative diseases, such as Alzheimer’s Disease and Parkinson’s Disease. In MSR deficient Drosophila melanogaster (fruit flies), larvae show a developmental delay like that seen when wild-type larvae are reared on nutrient deficit culture medium. These investigators further showed that serotonin levels were depressed in these nutrient deficient larvae....
Show moreMethionine sulfoxide reductase (MSR) is an important antioxidant to help mitigate oxidative stress that contributes to age-associated neurodegenerative diseases, such as Alzheimer’s Disease and Parkinson’s Disease. In MSR deficient Drosophila melanogaster (fruit flies), larvae show a developmental delay like that seen when wild-type larvae are reared on nutrient deficit culture medium. These investigators further showed that serotonin levels were depressed in these nutrient deficient larvae. The overarching aim of this study was to better understand the role of serotonin in MSR regulated physiology. Supplementing food with serotonin partially rescued the slower mouth hook movements (MHM) observed in the MSR-deficient flies. However, supplementation with serotonin altering drugs that cross the blood brain barrier (5-hydroxytryptophan, fluoxetine, or paravi chlorophenylalanine) did not rescue MHM and caused impairments to the growth of larvae during development. This study indicates that serotonin regulates feeding behavior partially through the regulation of MSR production but acts independently to regulate development.
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Date Issued: 2021
PURL: http://purl.flvc.org/fau/fd/FA00013761
Subject Headings: Drosophila melanogaster, Methionine sulfoxide reductase, Serotonin
Format: Document (PDF)

Title: The Effects of MsrA and MsrB in Anoxia Tolerance in Aging Drosophila melanogaster.
Creator: Suthakaran, Nirthieca, Binninger, David, Florida Atlantic University, Charles E. Schmidt College of Medicine, Department of Biomedical Sciences
Abstract/Description: Drosophila melanogaster tolerates several hours of anoxia (the absence of oxygen) by entering a protective coma. A burst of reactive oxygen species (ROS) is produced when oxygen is reintroduced to the cells. ROS causes oxidative damage to critical cellular molecules, which contribute to aging and development of certain agerelated conditions. The amino acid, methionine, is susceptible to oxidation, although this damage can be reversed by methionine sulfoxide reductases (Msr). This project...
Show moreDrosophila melanogaster tolerates several hours of anoxia (the absence of oxygen) by entering a protective coma. A burst of reactive oxygen species (ROS) is produced when oxygen is reintroduced to the cells. ROS causes oxidative damage to critical cellular molecules, which contribute to aging and development of certain agerelated conditions. The amino acid, methionine, is susceptible to oxidation, although this damage can be reversed by methionine sulfoxide reductases (Msr). This project investigates the effect of Msr-deficiency on anoxia tolerance in Drosophila throughout the lifespan of the animal. The data show that the time for recovery from the protective comma as well as the survival of the animals lacking any Msr activity depends on how quickly the coma is induced by the anoxic conditions. Insight into the roles(s) of Msr genes under anoxic stress can lead us to a path of designing therapeutic drugs around these genes in relation to stroke.
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Date Issued: 2018
PURL: http://purl.flvc.org/fau/fd/FA00013046
Subject Headings: Drosophila melanogaster, Methionine Sulfoxide Reductases, Anoxia, Aging, Oxidative stress
Format: Document (PDF)

Title: Reduced Reproductivity and Larval Locomotion in the Absence of Methionine Sulfoxide Reductase in Drosophila.
Creator: Singkornrat, Diana, Binninger, David, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: The inevitable aging process can be partially attributed to the accumulation of oxidative damage that results from the action of free radicals. Methionine sulfoxide reductases (Msr) are a class of enzymes that repair oxidized methionine residues. The two known forms of Msr are MsrA and MsrB which reduce the R- and S- enantiomers of methionine sulfoxide, respectively. Our lab has created the first genetic animal model that is fully deficient for any Msr activity. Previously our lab showed that...
Show moreThe inevitable aging process can be partially attributed to the accumulation of oxidative damage that results from the action of free radicals. Methionine sulfoxide reductases (Msr) are a class of enzymes that repair oxidized methionine residues. The two known forms of Msr are MsrA and MsrB which reduce the R- and S- enantiomers of methionine sulfoxide, respectively. Our lab has created the first genetic animal model that is fully deficient for any Msr activity. Previously our lab showed that these animals exhibit a 20 hour delay in development of the third instar larvae (unpublished data). My studies have further shown that the prolonged third-instar stage is due to a reduced growth rate associated with slower food intake and a markedly slower motility. These Msr-deficient animals also exhibit decreased egg-laying that can be attributed to a lack of female receptivity to mating.
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Date Issued: 2016
PURL: http://purl.flvc.org/fau/fd/FA00004777, http://purl.flvc.org/fau/fd/FA00004777
Subject Headings: Proteins--Chemical modification., Oxidative stress., Oxidation-reduction reaction., Drosophila melanogaster--Genetics., Mitochondrial pathology., Cellular signal transduction., Mutation (Biology), Aging--Molecular aspects.
Format: Document (PDF)

Title: Interfamilial relationships of Cactaceae within the dicot order, Caryophyllales.
Creator: Oliver, James Evans, Florida Atlantic University, Austin, Daniel F., Binninger, David, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: The position of the cactus family, Cactaceae, within the order Caryophyllales was examined by outgroup analysis of chloroplast rbcL gene sequence data. Comparative data came from 28 outgroup species in 15 families and six ingroup species. Phylogenetic Analysis with PAUP 3.1 produced twelve equally parsimonious trees; these were used to generate a strict consensus tree. MacClade 3.06 was used to refine the polytomies in the consensus tree. Analysis indicates that the Cactaceae is a...
Show moreThe position of the cactus family, Cactaceae, within the order Caryophyllales was examined by outgroup analysis of chloroplast rbcL gene sequence data. Comparative data came from 28 outgroup species in 15 families and six ingroup species. Phylogenetic Analysis with PAUP 3.1 produced twelve equally parsimonious trees; these were used to generate a strict consensus tree. MacClade 3.06 was used to refine the polytomies in the consensus tree. Analysis indicates that the Cactaceae is a monophyletic clade; moreover, these results support the work of other analyses that the sister family to Cactaceae is the Portulacaceae. Other relationships within the order are not completely consistent with previous studies. For example Molluginaceae and Caryophyllaceae occupy a central position in the cladogram; Achatocarpaceae aligns with the Amaranthaceae and Chenopodiaceae; and Basellaceae is presented as the basal family within the order. Lastly, the Jamaican cactus Opuntia spinosissima was derived from a common ancestor with the Florida Keys endemic, Opuntia corallicola.
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Date Issued: 1998
PURL: http://purl.flvc.org/fcla/dt/15593
Subject Headings: Caryophyllales--Analysis, Cactus--Analysis
Format: Document (PDF)

FAU Theses and Dissertations (14)	+ -
FAU Undergraduate Student Research (4)	+ -
FAU College Collections (2)	+ -
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Oxidative stress (5)	+ -
Drosophila melanogaster (4)	+ -
Cellular signal transduction (3)	+ -
Methionine Sulfoxide Reductases (3)	+ -
Mitochondrial pathology (3)	+ -

Mutation (Biology) (3)	+ -
Aging -- Molecular aspects (2)	+ -
Anoxia (2)	+ -
College students --Research --United States. (2)	+ -
Drosophila melanogaster -- Genetics (2)	+ -
Electric lines--Health aspects (2)	+ -
Electromagnetic fields--Health aspects (2)	+ -
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