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Title: DEVELOPING PLASMA-BASED DIAGNOSTICS: FROM PANCREATIC DUCTAL ADENOCARCINOMA TO HIV.
Creator: Makler, Amy, Asghar, Waseem, Florida Atlantic University, Department of Biological Sciences
Abstract/Description: Plasma-based diagnostics are ideal for detecting a variety of diseases because they offer a method of detection that is minimally invasive, readily available, and easy to use for monitoring patients as they progress through a disease or respond to treatment. The only serum marker for PDAC is CA19-9 which lacks specificity, has limited sensitivity, and is unreliable for early detection. It is therefore of great importance to develop a diagnostic that is viable for screening and early detection...
Show morePlasma-based diagnostics are ideal for detecting a variety of diseases because they offer a method of detection that is minimally invasive, readily available, and easy to use for monitoring patients as they progress through a disease or respond to treatment. The only serum marker for PDAC is CA19-9 which lacks specificity, has limited sensitivity, and is unreliable for early detection. It is therefore of great importance to develop a diagnostic that is viable for screening and early detection. Exosomal miRNA were determined via bioinformatics analyses and then examined in PDAC cell lines to identify markers with greatest potential. These markers were then examined in plasma from PDAC patients and control groups. Four markers, miR-93-5p, miR-339-3p, miR-425-5p, and miR-425-3p, emerged as the most viable biomarker panel with the ability to detect PDAC in 100% of the early stages (N=5) compared to CA19-9 which showed increased levels in only one patient with early stage PDAC. Additionally, the diagnostic has a specificity of 80% and a sensitivity of 94.7%, making it comparable to CA19-9, and may even be beneficial for use in conjunction with CA19-9. A plasma-based diagnostic was also developed for multi-strain HIV-1 detection utilizing the loop-mediated isothermal amplification (LAMP) method. LAMP primers were developed against the integrase and vpr regions of the HIV-1 genome. They were tested first in cultured HIV samples and then examined for their ability to amplify HIV-1 subtypes A-G. The integrase primer set provided a reliable means of diagnosing all 55 strains and isolates in under 30 minutes, whereas vpr was inconsistent and exhibited high variability in detecting the HIV subtypes. Our limit of detection for B-subtype with integrase was 30 viral copies/reaction. This could provide the basis for a novel, point-of-care diagnostic for use in underdeveloped regions.
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Date Issued: 2023
PURL: http://purl.flvc.org/fau/fd/FA00014141
Subject Headings: Plasma, Biomarkers, Carcinoma, Pancreatic Ductal, HIV-1, Diagnosis
Format: Document (PDF)

Title: Engineering cancer microenvironments for in vitro 3-D tumor models.
Creator: Asghar, Waseem, El Assal, Rami, Shafiee, Hadi, Pitteri, Sharon, Paulmurugan, Ramasamy, Demirci, Utkan
Date Issued: 2015-12
PURL: http://purl.flvc.org/fau/flvc_fau_islandoraimporter_10.1016_j.mattod.2015.05.002_1630594119
Format: Citation

Title: Engineering long shelf life multi-layer biologically active surfaces on microfluidic devices for point of care applications.
Creator: Asghar, Waseem, Yuksekkaya, Mehmet, Shafiee, Hadi, Zhang, Michael, Ozen, Mehmet O., Inci, Fatih, Kocakulak, Mustafa, Demirci, Utkan
Date Issued: 2016-08-17
PURL: http://purl.flvc.org/fau/fd/FAUIR000118
Format: Citation

Title: Biological Computation: the development of a genomic analysis pipeline to identify cellular genes modulated by the transcription / splicing factor srsf1.
Creator: Clark, Evan, Asghar, Waseem, Florida Atlantic University, College of Engineering and Computer Science, Department of Computer and Electrical Engineering and Computer Science
Abstract/Description: SRSF1 is a widely expressed mammalian protein with multiple functions in the regulation of gene expression through processes including transcription, mRNA splicing, and translation. Although much is known of SRSF1 role in alternative splicing of specific genes little is known about its functions as a transcription factor and its global effect on cellular gene expression. We utilized a RNA sequencing (RNA-¬‐Seq) approach to determine the impact of SRSF1 in on cellular gene expression and...
Show moreSRSF1 is a widely expressed mammalian protein with multiple functions in the regulation of gene expression through processes including transcription, mRNA splicing, and translation. Although much is known of SRSF1 role in alternative splicing of specific genes little is known about its functions as a transcription factor and its global effect on cellular gene expression. We utilized a RNA sequencing (RNA-¬‐Seq) approach to determine the impact of SRSF1 in on cellular gene expression and analyzed both the short term (12 hours) and long term (48 hours) effects of SRSF1 expression in a human cell line. Furthermore, we analyzed and compared the effect of the expression of a naturally occurring deletion mutant of SRSF1 (RRM12) to the full-¬‐length protein. Our analysis reveals that shortly after SRSF1 is over-¬‐expressed the transcription of several histone coding genes is down-¬‐regulated, allowing for a more relaxed chromatin state and efficient transcription by RNA Polymerase II. This effect is reversed at 48 hours. At the same time key genes for the immune pathways are activated, more notably Tumor Necrosis Factor-¬‐Alpha (TNF-¬‐α), suggesting a role for SRSF1 in T cell functions.
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Date Issued: 2017
PURL: http://purl.flvc.org/fau/fd/FA00004858, http://purl.flvc.org/fau/fd/FA00004858
Subject Headings: Gene expression., Computational biology., Markov processes., Bioinformatics., Genetic engineering., Molecular biology.
Format: Document (PDF)

Title: CEREBROSPINAL FLUID SHUNT SYSTEM WITH AUTO-FLOW REGULATION.
Creator: Mutlu, Caner, Asghar, Waseem, Florida Atlantic University, Department of Computer and Electrical Engineering and Computer Science, College of Engineering and Computer Science
Abstract/Description: A cerebrospinal fluid (CSF) shunt system is used for treatment of hydrocephalus and abnormal intracranial pressure (ICP) conditions. Mostly a shunt system is placed under skin for creating a low resistance pathway between intracranial space and appropriate discharge sites within body by doing so excess CSF volume can exit the intracranial space. Displaced intracranial CSF volume normally results in lowered ICP. Thereby, a CSF shunt can manage ICP. In a healthy person, normal ICP is primarily...
Show moreA cerebrospinal fluid (CSF) shunt system is used for treatment of hydrocephalus and abnormal intracranial pressure (ICP) conditions. Mostly a shunt system is placed under skin for creating a low resistance pathway between intracranial space and appropriate discharge sites within body by doing so excess CSF volume can exit the intracranial space. Displaced intracranial CSF volume normally results in lowered ICP. Thereby, a CSF shunt can manage ICP. In a healthy person, normal ICP is primarily maintained by CSF production and reabsorption rate as a natural tendency of body. If intracranial CSF volume starts increasing due to under reabsorption, this mostly results in raised ICP. Abnormal ICP can be treated by discharging excess CSF volume via use of a shunt system. Once a shunt system is placed subcutaneously, a patient is expected to live a normal life. However, shunt failure as well as flow regulatory problems are major issues with current passive shunt systems which leaves patients with serious consequences of under-/over CSF drainage condition. In this research, a shunt system is developed which is resistant to most shunt-related causes of under-/over CSF drainage. This has been made possible via use of an on-board medical monitoring (diagnostic) and active flow control mechanism. The developed shunt system, in this research, has full external ventricular drainage (EVD) capability. Further miniaturization will make it possible for an implantable shunt.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013489
Subject Headings: Cerebrospinal Fluid Shunts
Format: Document (PDF)

Title: DEVELOPMENT OF POINT-OF-CARE ASSAYS FOR DISEASE DIAGNOSTIC AND TREATMENT MONITORING FOR RESOURCE CONSTRAINED SETTINGS.
Creator: Sher, Mazhar, Asghar, Waseem, Florida Atlantic University, Department of Computer and Electrical Engineering and Computer Science, College of Engineering and Computer Science
Abstract/Description: This thesis aims to address the challenges of the development of cost-effective and rapid assays for the accurate counting of CD4+ T cells and quantification of HIV-1 viral load for resource-constrained settings. The lack of such assays has severely affected people living in disease prevalent areas. CD4+ T cells count information plays a vital role in the effective management of HIV-1 disease. Here, we present a flow-free magnetic actuation platform that uses antibody-coated magnetic beads to...
Show moreThis thesis aims to address the challenges of the development of cost-effective and rapid assays for the accurate counting of CD4+ T cells and quantification of HIV-1 viral load for resource-constrained settings. The lack of such assays has severely affected people living in disease prevalent areas. CD4+ T cells count information plays a vital role in the effective management of HIV-1 disease. Here, we present a flow-free magnetic actuation platform that uses antibody-coated magnetic beads to efficiently capture CD4+ T cells from a 30 μL drop of whole blood. On-chip cell lysate electrical impedance spectroscopy has been utilized to quantify the isolated CD4 cells. The developed assay has a limit of detection of 25 cells per μL and provides accurate CD4 counts in the range of 25–800 cells per μL. The whole immunoassay along with the enumeration process is very rapid and provides CD4 quantification results within 5 min time frame. The assay does not require off-chip sample preparation steps and minimizes human involvement to a greater extent. The developed impedance-based immunoassay has the potential to significantly improve the CD4 enumeration process especially for POC settings.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013495
Subject Headings: Point-of-care testing, Diagnostic tests, Immunoassay, HIV-1, Microfluidic devices
Format: Document (PDF)

Title: Development of Smart Phone-based Automated Microfluidic-ELISA For Human Immunodefciency Virus 1.
Creator: Coarsey, Chad Thomas, Asghar, Waseem, Florida Atlantic University, College of Engineering and Computer Science, Department of Computer and Electrical Engineering and Computer Science
Abstract/Description: The majority of HIV prevalence is found in Sub-Saharan Africa with 36.9 mil- lion living with HIV/AIDS. The cultural implications such as patient non-compliance or denial of available routine medical care can potentially cause limitations on the ef- fectiveness of detecting such virulent pathogens and manage chronic disease. The lack of access to healthcare and further socioeconomic impacts hinder the ability to ade- quately diagnose and treat infection in resource-limited settings....
Show moreThe majority of HIV prevalence is found in Sub-Saharan Africa with 36.9 mil- lion living with HIV/AIDS. The cultural implications such as patient non-compliance or denial of available routine medical care can potentially cause limitations on the ef- fectiveness of detecting such virulent pathogens and manage chronic disease. The lack of access to healthcare and further socioeconomic impacts hinder the ability to ade- quately diagnose and treat infection in resource-limited settings. Intervention through diagnosis and treatment helps prevent the spread of transmission, where pre-exposure prophylaxis or active disease prevention measures are not readily available. The cur- rent gold standard for HIV detection is by molecular detection; Reverse-Transcription Polymerase Chain Reaction is widely used that employs cycles of temperature condi- tions that require a thermal cycling platform and typically laboratory space for RNA extraction separate from RT-PCR space required. Serological detection can be ad- vantageous for surveillance and screening, Lateral Flow Assays and Enzyme-Linked Immunosorbent Assay (ELISA) can detect a viral protein (antigen) or antibodies. The ELISA can require at least 12 hours of assay preparation and takes a diagnostic laboratory many resources to run. There is need to develop Point-of-Care (POC) testing that can potentially be used for decentralized testing that can leverage ex- isting technologies such as smart phone capability and routine medical or diagnostic tests with cutting edge applications leveraging micro uidics, nanotechnology and in- tegrated circuit design. Such technologies allow for automated, rapid turnaround and cost-e ective diagnosis of HIV, where these assays could potentially be read- ily deployed. It is such technology that can potentially change the way diagnostics are performed, as POC technology can be rapidly disseminated, enable decentralized testing and, is user-friendly. A novel smart phone-enabled automated magnetic bead- based platform was developed for a micro uidic ELISA for HIV-1 detection at the POC to meet this demand.
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Date Issued: 2017
PURL: http://purl.flvc.org/fau/fd/FA00005945
Subject Headings: Dissertations, Academic -- Florida Atlantic University
Format: Document (PDF)

Title: GENOTYPIC SPERM SORTING: A less invasive “ART” to prevent Genetic Disorders in Newborns.
Creator: Adenmosun, Olumide O., Kumi-Diaka, James, Asghar, Waseem, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Genetic disorders like Cystic Fibrosis (CF) and X-linked Diseases (XLD) are inherited by offspring from parents who are healthy carriers of the autosomal recessive or allosomal genes. About 10-million Americans are healthy carriers of a mutant cysticfibrosis gene (predominantly F508del) and about 4% of newborns are at risk of being born with an X-linked disease. The current clinically approved mitigation plan for preventing genetic disorders in newborns from “at-risk couples” is to consider...
Show moreGenetic disorders like Cystic Fibrosis (CF) and X-linked Diseases (XLD) are inherited by offspring from parents who are healthy carriers of the autosomal recessive or allosomal genes. About 10-million Americans are healthy carriers of a mutant cysticfibrosis gene (predominantly F508del) and about 4% of newborns are at risk of being born with an X-linked disease. The current clinically approved mitigation plan for preventing genetic disorders in newborns from “at-risk couples” is to consider Preimplantation Genetic Testing for Monogenetic diseases (PGT-M). PGT-M involves an invasive microsurgical procedure that requires the removal of cells from 3-5day old embryos. To minimize this invasiveness, we proposed a less invasive approach to prevent genetic disorders in newborns by genotypically sorting sperm cells which may be used for fertilization events (IUI/IVF/ICSI) with specially characterized antigens on the sperm surface membrane. For the disease models being adopted in our study – CF and XLD; we utilized certain monoclonal antibodies (mab) to target the H-Y male antigen and the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein which are both selectively expressed on the sperm surface.
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Date Issued: 2021
PURL: http://purl.flvc.org/fau/fd/FA00013805
Subject Headings: Genetic disorders--Prevention, Genetic Testing, Reproductive technology, Cystic fibrosis
Format: Document (PDF)

Title: The Effect of Fluid Flow on Human Sperm Fertility.
Creator: Rappa, Kari, Asghar, Waseem, Florida Atlantic University, College of Engineering and Computer Science, Department of Computer and Electrical Engineering and Computer Science
Abstract/Description: Current sperm processing methods used in assisted reproductive technologies can cause damage to the sperm cell. New ways that mimic the natural guidance mechanisms present in the female genital tract may offer ways to sort sperm with better fertility parameters. Sperm that respond to these cues may have better over sperm health. Human sperm exhibit positive rheotaxis by orienting and swimming against the fluid released by the female genital tract. At certain flow rates sperm can actively...
Show moreCurrent sperm processing methods used in assisted reproductive technologies can cause damage to the sperm cell. New ways that mimic the natural guidance mechanisms present in the female genital tract may offer ways to sort sperm with better fertility parameters. Sperm that respond to these cues may have better over sperm health. Human sperm exhibit positive rheotaxis by orienting and swimming against the fluid released by the female genital tract. At certain flow rates sperm can actively orient and swim against the flow. Sperm retrieved that exhibit positive rheotaxis have higher motility and better morphology than the original semen sample.
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Date Issued: 2017
PURL: http://purl.flvc.org/fau/fd/FA00004938, http://purl.flvc.org/fau/fd/FA00004938
Subject Headings: Human reproduction--Immunological aspects., Fertility, Human., Cell separation., Reproductive health., Cilia and ciliary motion., Spermatozoa--Motility.
Format: Document (PDF)

Title: SMARTPHONE BASED SICKLE CELL DISEASE DETECTION AND ITS TREATMENT MONITORING FOR POINT-OF-CARE SETTINGS.
Creator: Ilyas, Shazia, Asghar, Waseem, Florida Atlantic University, Department of Computer and Electrical Engineering and Computer Science, College of Engineering and Computer Science
Abstract/Description: The majority of Sickle Cell Disease (SCD) prevalence is found in Sub-Saharan Africa, where 80% of the world’s population who suffer from this disease are born. Due to a lack of diagnosis and early treatments, 50-90% of these children will die before they reach the age of five. Current methods used for diagnosing SCD are based on hemoglobin analysis such as capillary electrophoresis, ion-exchange high-performance liquid chromatography, and isoelectric focusing. They require expensive...
Show moreThe majority of Sickle Cell Disease (SCD) prevalence is found in Sub-Saharan Africa, where 80% of the world’s population who suffer from this disease are born. Due to a lack of diagnosis and early treatments, 50-90% of these children will die before they reach the age of five. Current methods used for diagnosing SCD are based on hemoglobin analysis such as capillary electrophoresis, ion-exchange high-performance liquid chromatography, and isoelectric focusing. They require expensive laboratory equipment and are not feasible in these low-resource countries. It is, therefore, imperative to develop an alternative and cost-effective method for diagnosing and monitoring of SCD. This thesis aims to address the development and evaluation of a smartphone-based optical setup for the detection of SCD. This innovative technique can potentially be applied for low cost and accurate diagnosis of SCD and improve disease management in resource-limited settings where the disease exhibits a high prevalence. This Point-of-Care (POC) based device offers the potential to improve SCD diagnosis and patient care by providing a portable and cost effective device that requires minimal training to operate and analyze.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013475
Subject Headings: Anemia, Sickle Cell, Point-of-Care Systems, Sickle cell anemia--Treatment, Sickle cell anemia--Diagnosis, Smartphones
Format: Document (PDF)

Title: THE DEVELOPMENT OF WIDE-FIELD BIO-IMAGING FOR POINT OF CARE DIAGNOSTIC ASSAYS IN RESOURCE CONSTRAINED SETTINGS.
Creator: Fennell, Robert, Asghar, Waseem, Florida Atlantic University, Department of Computer and Electrical Engineering and Computer Science, College of Engineering and Computer Science
Abstract/Description: The World Health Organization has identified the need for affordable, specific, rapid and deliverable point of care assays for infectious diseases in areas that are resource poor and lacking readily available complex testing methods. The objective of this research is to discover improved methods of capturing and counting CD4+T, in a portable assay to aid in the detection of HIV or other diseases that are informed by cell identification and count. The research divides into 4 major objectives:...
Show moreThe World Health Organization has identified the need for affordable, specific, rapid and deliverable point of care assays for infectious diseases in areas that are resource poor and lacking readily available complex testing methods. The objective of this research is to discover improved methods of capturing and counting CD4+T, in a portable assay to aid in the detection of HIV or other diseases that are informed by cell identification and count. The research divides into 4 major objectives: Design an improved portable, microchip. to isolate cells in a timely manner. Explore, design and prove the optical technology that provides large field-of-view and enables imaging large surface area simultaneously so that a sufficient sample can be collected. Test and analyze the microchip and optics to verify the specificity and efficiency of the biological process. Identify and count the cells in an automated manner.
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Date Issued: 2021
PURL: http://purl.flvc.org/fau/fd/FA00013817
Subject Headings: Point-of-care testing, HIV
Format: Document (PDF)

Title: DEVELOPMENT OF POINT-OF-CARE ASSAYS FOR ZIKA VIRUS DIAGNOSTIC.
Creator: Md Alamgir Kabir, Asghar, Waseem, Florida Atlantic University, Department of Computer and Electrical Engineering and Computer Science, College of Engineering and Computer Science
Abstract/Description: Zika virus (ZIKV) is an emerging flavivirus transmitted to humans by Aedes mosquitos. ZIKV can be transmitted from mother to fetus during pregnancy and can cause microcephaly and other birth defects. Effective vaccines for Zika are yet to be approved. Detection of the ZIKV is based on serological testing that often shows cross-reactivity with the Dengue virus (DENV) and other flaviviruses. Currently, identification of ZIKV infection is usually done by i) testing the patient’s serum sample to...
Show moreZika virus (ZIKV) is an emerging flavivirus transmitted to humans by Aedes mosquitos. ZIKV can be transmitted from mother to fetus during pregnancy and can cause microcephaly and other birth defects. Effective vaccines for Zika are yet to be approved. Detection of the ZIKV is based on serological testing that often shows cross-reactivity with the Dengue virus (DENV) and other flaviviruses. Currently, identification of ZIKV infection is usually done by i) testing the patient’s serum sample to detect ZIKV RNA using reverse transcriptase-polymerase chain reaction (RT-PCR), ii) testing patient’s serum sample for the presence of the NS1 protein antigen or iii) serological assays to determine the presence of virus-specific immunoglobin antibodies (IgG and IgM) by the use of ELISA assay. But ELISA-based assays show cross-reactivity and poor sensitivity. The gold standard for ZIKV RNA detection is RT-PCR, involves expensive medical facilities and skillful technicians. However, the plaque reduction neutralization test are executed to quantity neutralizing antibodies of the virus-but show high accuracy only after day 7 of the disease onset. Therefore, the development of POC assays which has the ASSURED (affordability, sensitivity, specificity, user-friendly, rapid and robust, equipment-free and deliverable) criteria defined by the World Health Organization are topmost priority. The core objective of this thesis is to find inexpensive, sensitive, precise, and fast assays for the specific diagnosis of ZIKV suitable for resource-constrained settings.
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Date Issued: 2021
PURL: http://purl.flvc.org/fau/fd/FA00013836
Subject Headings: Point-of-care testing, Zika virus, Microfluidic devices
Format: Document (PDF)

Title: DEVELOPMENT OF A MICROFLUIDIC DEVICE FOR EXOSOME ISOLATION IN POINT-OF-CARE SETTINGS.
Creator: Ramnauth, Natasha, Waseem Asghar, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Exosomes have gained recognition in cancer diagnostics and therapeutics. Most exosome isolation methods are time-consuming, costly and require bulky equipment, rendering them unsuitable for point-of-care (POC) settings. Microfluidics can be the key to solving these challenges. Here, we employ the development of a double filtration microfluidic device that can rapidly isolate exosomes in POC settings. The device can efficiently isolate exosomes from just 100 uL of plasma within 50 minutes. The...
Show moreExosomes have gained recognition in cancer diagnostics and therapeutics. Most exosome isolation methods are time-consuming, costly and require bulky equipment, rendering them unsuitable for point-of-care (POC) settings. Microfluidics can be the key to solving these challenges. Here, we employ the development of a double filtration microfluidic device that can rapidly isolate exosomes in POC settings. The device can efficiently isolate exosomes from just 100 uL of plasma within 50 minutes. The device was compared against Polyethylene glycol (PEG) based precipitation, and findings show that both methods yield comparable exosome sizes and purity, but the device can detect exosomal miRNA earlier than PEG. Finally, a comparative analysis of membrane filters with exosomes collected from pore sizes 15 nm and 30 nm showed a similarity in exosome size and miRNA expressions, with significantly increased sample purity. These findings suggest that this device has potential in POC settings.
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Date Issued: 2022
PURL: http://purl.flvc.org/fau/fd/FA00014071
Subject Headings: Microfluidic devices, Exosomes, Point-of-care testing
Format: Document (PDF)

Title: DEVELOPMENT OF AN AUTOMATED DEVICE FOR THE OPTIMIZED REGULATION OF CEREBROSPINAL FLUID (CSF).
Creator: Anjum, Muhammad Waleed, Asghar, Waseem, Florida Atlantic University, Department of Computer and Electrical Engineering and Computer Science, College of Engineering and Computer Science
Abstract/Description: Cerebrospinal fluid (CSF) has a role, in keeping the brain and spinal cord safe and nourished within the nervous system (CNS). This clear and colorless fluid is produced in the ventricles of the brain. Surrounds these structures acting as a protective cushion. CSF plays a role in maintaining nervous system health and ensuring optimal functioning. CSF accomplishes four objectives. Protection: The brain and spinal cord are shielded from harm due to CSFs natural shock absorbing properties. This...
Show moreCerebrospinal fluid (CSF) has a role, in keeping the brain and spinal cord safe and nourished within the nervous system (CNS). This clear and colorless fluid is produced in the ventricles of the brain. Surrounds these structures acting as a protective cushion. CSF plays a role in maintaining nervous system health and ensuring optimal functioning. CSF accomplishes four objectives. Protection: The brain and spinal cord are shielded from harm due to CSFs natural shock absorbing properties. This effectively safeguards these structures, from injuries caused by impacts or collisions. Nutrition It ensures a favorable environment for neural cells to perform at their peak by supplying essential nutrients and removing waste products from the brain and spinal cord.
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Date Issued: 2023
PURL: http://purl.flvc.org/fau/fd/FA00014342
Subject Headings: Cerebrospinal fluid, Biomedical devices, Biomedical engineering
Format: Document (PDF)

Title: DEVELOPMENT OF MICROFLUIDIC PLATFORMS FOR INFECTIOUS DISEASES DIAGNOSIS AND SPERM CELL SORTING.
Creator: Sharma, Sandhya, Asghar, Waseem, Florida Atlantic University, Department of Computer and Electrical Engineering and Computer Science, College of Engineering and Computer Science
Abstract/Description: In recent years, point-of-care (POC) microfluidic platforms have transformed the healthcare landscape as they offer rapid, low-cost, and easy operational benefits. POC diagnostics play an important role in expediting the testing process in resource-constrained areas. These platforms have become a powerful tool as they offer comparable results with gold-standard methods. The gold standard methods require sophisticated lab locations and expensive equipment, to process the samples which is a...
Show moreIn recent years, point-of-care (POC) microfluidic platforms have transformed the healthcare landscape as they offer rapid, low-cost, and easy operational benefits. POC diagnostics play an important role in expediting the testing process in resource-constrained areas. These platforms have become a powerful tool as they offer comparable results with gold-standard methods. The gold standard methods require sophisticated lab locations and expensive equipment, to process the samples which is a significant challenge particularly for people living in low-income countries. To address these limitations, herein, in my dissertation, I have developed POC microfluidic platforms that can be operated outside the laboratory using lesser equipment statistically hence reducing the testing cost and time. The developed POC chips are used for infectious diseases diagnosis for viruses such as Zika, Hepatitis C Virus (HCV), and severe acute respiratory syndrome coronavirus 2 (SARSCoV-2). The entire virus detection process was executed inside a uniquely designed, inexpensive, disposable self-driven microfluidic chip with high sensitivity and specificity. In addition to this, I have also developed a microfluidic platform for functional sperm cell sorting from raw semen samples. The microfluidic chip offers a platform where the sperm cells experience different shear stress in different parts of the chip that facilitates isolation of competent sperm cells without impacting their integrity. Simultaneously, it also allows effortless collection of sorted sperm cells from the collection chamber which holds clinical significance. All things considered, the developed devices are inexpensive, disposable, easy-to-use, and rapid that provide results within one hour.
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Date Issued: 2022
PURL: http://purl.flvc.org/fau/fd/FA00013906
Subject Headings: Microfluidics, Point-of-care testing, Communicable diseases—Diagnosis
Format: Document (PDF)

Title: Paper and Flexible Substrates as Materials for Biosensing Platforms to Detect Multiple Biotargets.
Creator: Shafiee, Hadi, Asghar, Waseem, Inci, Fatih, Yuksekkaya, Mehmet, Jahangir, Muntasir, Zhang, Michael H., Durmus, Naside Gozde, Gurkan, Umut Atakan, Kuritzkes, Daniel R., Demirci, Utkan
Date Issued: 2015-08-06
PURL: http://purl.flvc.org/fau/flvc_fau_islandoraimporter_10.1038_srep08719_1630600934
Format: Citation

Title: Toxicology Study of Single-walled Carbon Nanotubes and Reduced Graphene Oxide in Human Sperm.
Creator: Asghar, Waseem, Shafiee, Hadi, Velasco, Vanessa, Sah, Vasu R., Guo, Shirui, El Assal, Rami, Inci, Fatih, Rajagopalan, Adhithi, Jahangir, Muntasir, Anchan, Raymond M., Mutter, George L., Ozkan, Mihrimah, Ozkan, Cengiz S., Demirci, Utkan
Date Issued: 2016-08-19
PURL: http://purl.flvc.org/fau/flvc_fau_islandoraimporter_10.1038_srep30270_1630604252
Format: Citation

Title: DEVELOPMENT OF A WEARABLE DEVICE FOR MONITORING PHYSIOLOGICAL PARAMETERS RELATED TO HEART FAILURE.
Creator: Iqbal, Sheikh Muhammad Asher, Asghar, Waseem, Florida Atlantic University, Department of Computer and Electrical Engineering and Computer Science, College of Engineering and Computer Science
Abstract/Description: Heart failure is a chronic cardiovascular disease that is caused due to the lack of blood supply from heart. This lack of blood supply leads to accumulation of the fluid in the thoracic region. Traditionally, implantable cardioverter defibrillators (ICDs) are used to treat HF and to monitor its parameters. Healthcare wearable devices (HWDs) are healthcare devices that can be worn or attached to the skin. HWD are non-invasive and low-cost means of providing healthcare at the point-of-care (POC...
Show moreHeart failure is a chronic cardiovascular disease that is caused due to the lack of blood supply from heart. This lack of blood supply leads to accumulation of the fluid in the thoracic region. Traditionally, implantable cardioverter defibrillators (ICDs) are used to treat HF and to monitor its parameters. Healthcare wearable devices (HWDs) are healthcare devices that can be worn or attached to the skin. HWD are non-invasive and low-cost means of providing healthcare at the point-of-care (POC). Herein, this dissertation discusses the development of a HWD for the monitoring of the parameters of heart failure (HF). These parameters include thoracic impedance, electrocardiogram (ECG), heart rate, oxygen saturation in blood and activity status of the subject. These are similar parameters as monitored using ICD. The dissertation will discuss the development, design, and results of the HWD.
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Date Issued: 2023
PURL: http://purl.flvc.org/fau/fd/FA00014349
Subject Headings: Wearable technology--Design and construction, Wearable devices, Heart failure
Format: Document (PDF)

FAU Theses and Dissertations (14)	+ -
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Point-of-care testing (5)	+ -
Microfluidic devices (3)	+ -
HIV-1 (2)	+ -
Anemia, Sickle Cell (1)	+ -
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Biomedical devices (1)	+ -
Biomedical engineering (1)	+ -
Carcinoma, Pancreatic Ductal (1)	+ -
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