Current Search: Alluri, Ravi Kumar (x)
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Title
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Multiple factors determine tRNA 3' maturation by RNase R of mycoplasma genitalium.
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Creator
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Alluri, Ravi Kumar, Graduate College
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Date Issued
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2010-04-09
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PURL
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http://purl.flvc.org/fcla/dt/3176161
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Format
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Document (PDF)
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Title
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tRNA processing and quality control in bacteria.
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Creator
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Alluri, Ravi Kumar, Li, Zhongwei, Charles E. Schmidt College of Science, Department of Biomedical Science
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Abstract/Description
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In this work, we report that the only exoribonuclease in M. genitalium, RNase R, is able to generate mature 3'-ends. The aminoacyl-acceptor stem, CCA terminus and discriminator residue plays an important role in stopping RNase R digestion at the mature 3'-end. Disruption of the stem causes partial or complete degradation of the pre-tRNA, whereas extension of the stem results in the formation of a mature 3’-end. CC residues in CCA terminus and A or G residues at discriminator position are the...
Show moreIn this work, we report that the only exoribonuclease in M. genitalium, RNase R, is able to generate mature 3'-ends. The aminoacyl-acceptor stem, CCA terminus and discriminator residue plays an important role in stopping RNase R digestion at the mature 3'-end. Disruption of the stem causes partial or complete degradation of the pre-tRNA, whereas extension of the stem results in the formation of a mature 3’-end. CC residues in CCA terminus and A or G residues at discriminator position are the most preferred residues for precise stopping of RNase R at mature 3’ end. The significance of this works shows that M. genitalium RNase R generates mature tRNA in a single step by recognizing features in the terminal domains of tRNA, a process requiring multiple RNases in most bacteria.
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Date Issued
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2013
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PURL
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http://purl.flvc.org/fau/fd/FA00004174
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Format
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Document (PDF)
