Current Search: Fields, Gregg B. (x)
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Title
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Metalloprotease Profiling in Melanoma.
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Creator
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Giricz, Orsolya, Florida Atlantic University, Fields, Gregg B., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
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Abstract/Description
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The proteolytic activities of the ADAM (a disintegrin and metalloproteinase), ADAMTS (a disintegrin and metalloproteinases with thrombospondin motifs) and MMP (matrix metalloproteinase) protein families play important roles in normal and multiple pathological conditions. These metalloproteases have potential implications in the degradation of the extracellular matrix and in the processing of bioactive molecules. Under pathological conditions these proteases are involved in many diverse...
Show moreThe proteolytic activities of the ADAM (a disintegrin and metalloproteinase), ADAMTS (a disintegrin and metalloproteinases with thrombospondin motifs) and MMP (matrix metalloproteinase) protein families play important roles in normal and multiple pathological conditions. These metalloproteases have potential implications in the degradation of the extracellular matrix and in the processing of bioactive molecules. Under pathological conditions these proteases are involved in many diverse processes from tumor cell migration to cartilage destruction in rheumatoid arthritis. In the present study, the gene expression levels of six ADAMs, eight MMPs, and four ADAMTSs were analyzed by Real Time PCR. RNA was isolated from multiple normal fibroblast and metastatic melanoma cell lines, as well as the isogenic normal tissue and tumor samples. This method allowed for detected changes in mRNA expressiOn of the individual metalloproteainase genes to be compared between normal and metastatic states, and also between tissue and cultured cells. Substantial differences have been observed in the level of ADAM and MMP mRNA expression between tissue and cell lines. In general, the level of expression is several folds higher in cultured cells compared to the isogenic tissue they are derived from. Protein microarrays were utilized in order to evaluate the correlations between MMP and TIMP mRNA copy numbers and protein abundance in cell culture. In several cases, distinct differences were observed regarding the localization of the proteins examined. In order to determine if the metalloprotease genes that were elevated at the level of RNA expression produce functional proteins, the foundations of an in situ FRET assay have been established. This will greatly aid in a better understanding of the behavior of metallopeptidases in a cellular context.
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Date Issued
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2008
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PURL
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http://purl.flvc.org/fau/fd/FA00000859
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Subject Headings
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Tumor markers--Research, Metalloproteinases--Inhibitors, Melanoma--Research, Proteins--Synthesis, Genetic translation
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Format
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Document (PDF)
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Title
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The Roles of Substrate Thermal Stability and P2 and P1′ Subsite Identity on Matrix Metalloproteinase Triple-helical Peptidase Activity and Collagen Specificity.
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Creator
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Minond, Dmitriy, Lauer-Fields, Janelle L., Cudic, Mare, Overall, Christopher M., Pei, Duanqing, Brew, Keith, Visse, Robert, Nagase, Hideaki, Fields, Gregg B.
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Date Issued
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2006-12
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PURL
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http://purl.flvc.org/fau/flvc_fau_islandoraimporter_10.1074_jbc.M606004200_1638203001
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Format
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Document (PDF)
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Title
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The Mechanism by Which MYCN Amplification Confers an Enhanced Sensitivity to a PCNA-Derived Cell Permeable Peptide in Neuroblastoma Cells.
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Creator
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Gu, Long, Chu, Peiguo, Lingeman, Robert, McDaniel, Heather, Kechichian, Steven, Hickey, Robert J., Liu, Zheng, Yuan, Yate-Ching, Sandoval, John A., Fields, Gregg B., Malkas, Linda H.
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Date Issued
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2015-12
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PURL
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http://purl.flvc.org/fau/fd/FAUIR000140
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Format
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Citation
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