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Title: Characterization of genetic markers for in vitro cell line identification of the marine sponge, Axinella corrugata.
Creator: Lopez, Jose V., Peterson, C. L., Willoughby, Robin, Wright, Amy E., Enright, E., Zoladz, S., Pomponi, Shirley A.
Date Issued: 2002
PURL: http://purl.flvc.org/FCLA/DT/3355156
Subject Headings: Sponges--Research, Marine invertebrates, Genetic markers
Format: Document (PDF)

Title: Bioactivity of marine organisms: relationships with taxonomy, geography and depth.
Creator: Reed, John K., Sennett, Susan H., McCarthy, Peter J., Pitts, Tara P., Wright, Amy E., Pomponi, Shirley A.
Date Issued: 1998
PURL: http://purl.flvc.org/fau/fd/FA00007421
Subject Headings: Marine organisms, Bioactive compounds, Marine natural products
Format: Document (PDF)

Title: New Bioactive Peroxides from Marine Sponges of the Family Plakiniidae.
Creator: Chen, Ying, McCarthy, Peter J., Harmody, Dedra K., Schimoler, Rebecca, Chilson, Katherine, Selitrennikoff, Claude, Pomponi, Shirley A., Wright, Amy E.
Date Issued: 2002
PURL: http://purl.flvc.org/FCLA/DT/3164099
Subject Headings: Sponges, Peroxides, Pathogenic fungi, Candida albicans, Aspergillus fumigatus
Format: Document (PDF)

Title: Discorhabdin P, a new enzyme inhibitor from a deep-water Caribbean sponge of the genus Batzella.
Creator: Gunasekera, Sarath P., McCarthy, Peter J., Longley, Ross E., Pomponi, Shirley A., Wright, Amy E., Lobkovsky, E., Clardy, J.
Date Issued: 1999
PURL: http://purl.flvc.org/FCLA/DT/3319100
Subject Headings: Sponges --Research, Marine natural products, discorhabdin P, Enzyme inhibitors
Format: Document (PDF)

Title: Sesquiterpene-derived metabolites from the deep water marine sponge Poecillastra sollasi.
Creator: Killday, K. B., Longley, Ross E., McCarthy, Peter J., Pomponi, Shirley A., Wright, Amy E.
Date Issued: 1993
PURL: http://purl.flvc.org/FCLA/DT/3318952
Subject Headings: Sponges --Research, Sesquiterpenes, Marine natural products, Marine metabolites
Format: Document (PDF)

Title: (+)-Curcuphenol and (+)-Curcudiol, sesquiterpene phenols from shallow and deep water collections of the marine sponge Didiscus Flavus.
Creator: Wright, Amy E., Pomponi, Shirley A., McConnell, O. J., Kohmoto, S., McCarthy, Peter J.
Date Issued: 1987
PURL: http://purl.flvc.org/FCLA/DT/3332940
Subject Headings: Sponges, Sesquiterpenes, Antifungal agents, Marine metabolites
Format: Document (PDF)

Title: Lasonolides C—G, Five New Lasonolide Compounds from the Sponge Forcepia sp.
Creator: Wright, Amy E., Chen, Ying, Winder, Priscilla L., Pitts, Tara P., Pomponi, Shirley A., Longley, Ross E.
Date Issued: 2004
PURL: http://purl.flvc.org/FCLA/DT/3164109
Subject Headings: Macrolide antibiotics, Natural products, Mass spectrometry, Sponges, Stereochemistry
Format: Document (PDF)

Title: Diversity and bioactivity of marine sponges of the Caribbean: the Turks and Caicos Islands.
Creator: Pomponi, Shirley A., Reed, John K., Wright, Amy E., McCarthy, Peter J.
Date Issued: 1996
PURL: http://purl.flvc.org/FCLA/DT/3351966
Subject Headings: Sponges--Turks and Caicos Islands, Bioactive compounds, Marine natural products, Sponges, Demospongiae
Format: Document (PDF)

Title: A chemical view of the most ancient metazoa – biomarker chemotaxonomy of hexactinellid sponges.
Creator: Thiel, Volker, Blumenberg, Martin, Hefter, Jens, Pape, Thomas, Pomponi, Shirley A., Reed, John K., Reitner, Joachim, Worheide, Gert, Michaelis, Walter
Date Issued: 2002
PURL: http://purl.flvc.org/FCLA/DT/2709448
Subject Headings: Sponges --Microbiology
Format: Document (PDF)

Title: Conservation and Phylogeny of a Novel Family of Non‐Hox Genes of the Antp Class in Demospongiae (Porifera).
Creator: Richelle-Maurer, Evelyn, Boury-Esnault, Nicole, Itskovich, Valeria B., Manuel, Michael, Pomponi, Shirley A., Van de Vyver, Gisele, Borchiellini, Carole
Date Issued: 2006
PURL: http://purl.flvc.org/FCLA/DT/2796095
Subject Headings: Demospongiae, Homeobox genes, Marine ecosystem management, Phylogeny, Sponges --Bahamas, Sponges --Florida
Format: Document (PDF)

Title: Secobatzellines A and B, two new enzyme inhibitors from a deep-water Caribbean sponge of the genus Batzella.
Creator: Gunasekera, Sarath P., McCarthy, Peter J., Longley, Ross E., Pomponi, Shirley A., Wright, Amy E.
Date Issued: 1999
PURL: http://purl.flvc.org/FCLA/DT/3172682
Subject Headings: Sponges --Caribbean Sea, Marine natural products, Toxicity testing --In vitro, Phosphatases, Peptidase
Format: Document (PDF)

Title: Topsentin, bromotopsentin, and dihydrodeoxybromotopsentin; antiviral and antitumor bis(indolyl)imidazoles from Caribbean deep-sea sponges of the family Halichondriidae. Structural and synthetic studies.
Creator: Tsujii, S., Rinehart, Kenneth L., Gunasekera, Sarath P., Kashman, Y., Cross, S. S., Lui, M. S., Pomponi, Shirley A., Diaz, Maria Cristina
Date Issued: 1988
PURL: http://purl.flvc.org/FCLA/DT/3332952
Subject Headings: Sponges--Caribbean Sea, Imidazoles, Indoles, Antiviral agents, Antineoplastic agents
Format: Document (PDF)

Title: Cellular Basis of Sponge-Sponge Associations.
Creator: Conkling, Megan, Pomponi, Shirley A., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Marine sponges interact and coexist with many different organisms. A two-sponge association between Amphimedon erina and Geodia gibberosa commonly occurs in the Florida Keys. Previous studies have only focused on the ecological influence of the association; they did not examine the cellular basis of the association. This association between A. erina and G. gibberosa was used in the development of an in vitro model to further the understanding of the cellular basis of natural sponge-sponge...
Show moreMarine sponges interact and coexist with many different organisms. A two-sponge association between Amphimedon erina and Geodia gibberosa commonly occurs in the Florida Keys. Previous studies have only focused on the ecological influence of the association; they did not examine the cellular basis of the association. This association between A. erina and G. gibberosa was used in the development of an in vitro model to further the understanding of the cellular basis of natural sponge-sponge associations. In this study, sponge cells were cultured individually and in co-cultures and their responses related to apoptosis, cell death, and proliferation were monitored using high content imaging. Co-cultured cells of species that form sponge-sponge associations did not have the same cellular responses compared to co-cultured cells of species that do not form sponge-sponge associations. Protein expression analyses demonstrated that the model that was established does not mimic the cellular response of the association in nature, but this model can be used to test in vitro cellular interactions of sponge species that do not form associations in nature. In addition, the protein expression data that were obtained revealed that sponges use similar apoptotic pathways as humans and suggest that sponge cells may shut down cell cycling in order to repair damaged DNA. This research is a small piece to the puzzle that is sponge cell culture research.
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Date Issued: 2017
PURL: http://purl.flvc.org/fau/fd/FA00004848, http://purl.flvc.org/fau/fd/FA00004848
Subject Headings: Sponges--Habitat--Florida., Marine invertebrates--Florida., Aquatic invertebrates--Cultures and culture media., Apoptosis., Cell culture., Symbiosis.
Format: Document (PDF)

Title: Discovery of Loxosomella vivipara (Entoprocta: Loxosomatidae) in the Marine Sponge Hippospongia cf. gossypina (Porifera: Spongiidae) in the Florida Keys.
Creator: Plunkett, Rachel, Pomponi, Shirley A., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Populations of a marine invertebrate symbiont were found on the outer surface and internal spaces of a keratose sponge from a shallow bay in the Florida Keys in May 2014. A total of 24 specimens of the seagrass and reef-dwelling sponge were collected between May 2014 and August 2015 to provide material to identify both host and symbiont, and elucidate information on the nature of the association. Based on a morphological analysis via light microscopy, histology, and scanning electron...
Show morePopulations of a marine invertebrate symbiont were found on the outer surface and internal spaces of a keratose sponge from a shallow bay in the Florida Keys in May 2014. A total of 24 specimens of the seagrass and reef-dwelling sponge were collected between May 2014 and August 2015 to provide material to identify both host and symbiont, and elucidate information on the nature of the association. Based on a morphological analysis via light microscopy, histology, and scanning electron microscopy (SEM), and 99% similarity in aligned partial sequences from 28S and 18S nuclear ribosomal genes (rDNA), the symbiont was identified as the solitary entoproct Loxosomella vivipara Nielsen, 1966 (Entoprocta: Loxosomatidae). A partial sequence from the Internal Transcribed Spacer Region 2 (ITS2) of L. vivipara was registered to GenBank for the first time. The identity of the host sponge, based on a morphological investigation, is resolved as “velvet sponge” Hippospongia cf. gossypina Duchassing and Michelotti, 1864 (Demospongiae: Spongiidae). This is the first report of an entoproct commensal from Hippospongia cf. gossypina, a sponge that formerly had great commercial value when it was abundant throughout the Bahamas, Florida Keys and Gulf of Mexico. Other common sponge species at the study site were collected to investigate the host specificity of L. vivipara. Evidence that L. vivipara favorably selects the sponges Hippospongia cf. gossypina and Chondrilla nucula over other potential host sponges at the study site is provided. Commensalism is the most plausible justification for this relationship: L. vivipara is dependent on sponges for protection and food particles, while the sponges are unaffected by its presence. Further evidence of host-specific inquilinism is provided for L. vivipara associated with Hippospongia cf. gossypina, but not for L. vivipara associated with C. nucula. An inquilinistic association between an entoproct and sponge is a rare discovery only mentioned in one previous study. Sponge aquiferous qualities such as aperture and canal size, canal and choanocyte chamber arrangement, seawater pumping rate, and food particle size selection are likely factors that inhibit or enable inquilinism in sponge-entoproct associations – a potential topic for future researc
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Date Issued: 2016
PURL: http://purl.flvc.org/fau/fd/FA00004791, http://purl.flvc.org/fau/fd/FA00004791
Subject Headings: Marine invertebrates--Florida., Sponges--Habitat--Florida., Marine resources--Florida--Management., Aquatic biology., Entoprocta--Classification.
Format: Document (PDF)

Title: Morphological Diagnoses of Sponges Collected at Shelf-edge MPAs from Eastern Florida to North Carolina, between depths of 51 and 150 m, during CIOERT Expeditions (2018-2019).
Creator: Diaz Ronda, Maria Cristina, Farrington, Stephanie, Reed, John K., Pomponi, Shirley A.
Abstract/Description: This paper is a result of the taxonomic analyses of the sponges collected during the NOAA Ship Pisces expeditions in 2018 and 2019 with the University of North Carolina, Wilmington (UNCW) Mohawk ROV. This is part of a 9-year project conducted by NOAA National Marine Fisheries (NMF) in collaboration with the Cooperative Institute for Ocean Exploration, Research, and Technology (CIOERT) at Harbor Branch Oceanographic Institute, Florida Atlantic University (HBOI-FAU) to document and monitor the...
Show moreThis paper is a result of the taxonomic analyses of the sponges collected during the NOAA Ship Pisces expeditions in 2018 and 2019 with the University of North Carolina, Wilmington (UNCW) Mohawk ROV. This is part of a 9-year project conducted by NOAA National Marine Fisheries (NMF) in collaboration with the Cooperative Institute for Ocean Exploration, Research, and Technology (CIOERT) at Harbor Branch Oceanographic Institute, Florida Atlantic University (HBOI-FAU) to document and monitor the Marine Protected Areas (MPAs) of the southeastern U.S. continental shelf (eastern Florida to North Carolina, 50-165 m deep). The most prominent and conspicuous sponges were collected with the intention of evaluating the biodiversity of this important benthic group in these mesophotic habitats. Eleven sponge samples were collected, and taxonomic evaluation, studying the internal and external morphology, resulted in: 1) three well recognized species from the Tropical Western Atlantic (Leiodermatium lynceus, Neofibularia nolitangere, Clathria foliacea), 2) four species with sibling recognized species but with major morphological differences that might require the description of new species (Epipolasis cf. profunda, Cliona cf. tumula, Cliona aff. celata), and 3) three species that only could receive generic assignations (Penares sp.1, Clathria sp.1, and Aaptos sp.1). These results show that more than half of the species collected represent unknown species to science, or unknown variations of recognized species. Careful comparison with species types and observations of spicules under SEM would allow the necessary evidence to characterize and classify these new species or varieties. We suggest further characterization of these novel fauna considering their level of abundance and distribution in the MPAs studied. A synoptic description of each species studied, with live, deck and microscopy photographs is here presented.
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Date Issued: 2018 - 2019
PURL: http://purl.flvc.org/fau/fd/FAUIR000407
Format: Document (PDF)

Title: EVALUATION OF 3-D CULTURE METHODS FOR MARINE SPONGES.
Creator: Urban-Gedamke, Elizabeth, Pomponi, Shirley A., Florida Atlantic University, Department of Marine Science and Oceanography, Charles E. Schmidt College of Science
Abstract/Description: Marine sponges are economically and environmentally valuable, but restoration, commercial, and biomedical demands exceed what wild populations and aquaculture can provide. In vitro culture of sponge cells is a promising alternative, but has remained elusive until recent breakthroughs involving improved nutrient medium M1 in two-dimensional culture. The advantages of three-dimensional over two-dimensional cell culture have been increasingly recognized. Here we report the successful 21-day...
Show moreMarine sponges are economically and environmentally valuable, but restoration, commercial, and biomedical demands exceed what wild populations and aquaculture can provide. In vitro culture of sponge cells is a promising alternative, but has remained elusive until recent breakthroughs involving improved nutrient medium M1 in two-dimensional culture. The advantages of three-dimensional over two-dimensional cell culture have been increasingly recognized. Here we report the successful 21-day culture of cells from the marine sponge Geodia neptuni using multiple three-dimensional cell culture methods: FibraCel© disks, thin hydrogel layers, gel micro droplets, and spheroid cell culture. These methods performed comparably to two-dimensional control cultures, and each method offers advantages for restoration or in vitro applications using sponge cells. Further optimization of these methods may lead to the ability to culture fully functioning sponges from dissociated, cryopreserved cells, which will reduce the need for wild harvest of sponge tissues for commercial, restoration, and biomedical purposes.
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Date Issued: 2021
PURL: http://purl.flvc.org/fau/fd/FA00013726
Subject Headings: Sponges, Cell Culture--methods
Format: Document (PDF)

Title: ESTABLISHING A SPONGE HYBRIDOMA FOR THE IN VITRO PRODUCTION OF A MARINE NATURAL PRODUCT.
Creator: Dougan, Cassady, Pomponi, Shirley A., Florida Atlantic University, Department of Marine Science and Oceanography, Charles E. Schmidt College of Science
Abstract/Description: Marine sponges are one of the most prolific sources of chemical compounds with pharmaceutical importance. To establish a supply of such compounds large enough for clinical development, in vitro production methodology was investigated. Since all sponge cells do not divide in culture, it was hypothesized that the fusion of rapidly dividing cells of a sponge that does not produce any compounds of interest with cells of a nondividing but compound-producing sponge would result in a hybridoma that...
Show moreMarine sponges are one of the most prolific sources of chemical compounds with pharmaceutical importance. To establish a supply of such compounds large enough for clinical development, in vitro production methodology was investigated. Since all sponge cells do not divide in culture, it was hypothesized that the fusion of rapidly dividing cells of a sponge that does not produce any compounds of interest with cells of a nondividing but compound-producing sponge would result in a hybridoma that produces the compound of interest. In this study, hybridomas have been produced with cells of two marine sponges, Axinella corrugata, which produces the antitumor compound stevensine, and Geodia neptuni, which divides rapidly in a nutrient medium optimized for sponge cell culture. Successful hybridization and subsequent cell division and in vitro stevensine production may unlock the potential for sustainable mass production of other sponge-derived compounds.
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Date Issued: 2022
PURL: http://purl.flvc.org/fau/fd/FA00014024
Subject Headings: Marine natural products, Sponges, Hybridomas, In vitro
Format: Document (PDF)

Title: REEF WARS: MONITORING AND PREDICTING PHASE SHIFTS ON FLORIDA CORAL REEFS.
Creator: Conkling, Megan, Pomponi, Shirley A., Hindle, Tobin, Florida Atlantic University, Department of Geosciences, Charles E. Schmidt College of Science
Abstract/Description: Reefs off the coast of Florida face threats from stressors associated with climate change which leads to phase shifts. Under rapid climate change, a clear understanding of how reefs and their benthic organisms respond is still lacking and needs to be investigated. Using in situ imagery, a sponge cell model, and long-term benthic biota surveys, the effects of climate change on reef dynamics were explored in this dissertation project. Results from the in situ imagery found that differences in...
Show moreReefs off the coast of Florida face threats from stressors associated with climate change which leads to phase shifts. Under rapid climate change, a clear understanding of how reefs and their benthic organisms respond is still lacking and needs to be investigated. Using in situ imagery, a sponge cell model, and long-term benthic biota surveys, the effects of climate change on reef dynamics were explored in this dissertation project. Results from the in situ imagery found that differences in spectral signatures are found between functional groups (i.e., corals, sponges, and algae) and different species from substrate. Results based on a sponge cell model and transcriptomics data have found a resilience of these sponges to the predicted thermal extremes. Results from benthic biota surveys suggested that depth and light attenuation have the largest influence on the predicted distribution of corals, sponges, and algae at Pulley Ridge. Climate change has been impacting reef benthic biota starting at the organismal scale up to the reef scale. This research demonstrates the importance of monitoring reefs at a finer scale and determining the thresholds and limits of benthic biota to projected thermal extremes to better inform resource managers to preserve these irreplaceable ecosystems.
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Date Issued: 2023
PURL: http://purl.flvc.org/fau/fd/FA00014323
Subject Headings: Reefs--Florida, Climate change, Coral reef ecology
Format: Document (PDF)

Title: Neopeltolide a new macrolide from a lithistid sponge of the family Neopeltidae.
Creator: Wright, Amy E., Botelho, J. C., Guzman, Esther A., Harmody, Dedra K., Linley, P. A., McCarthy, Peter J., Pitts, Tara P., Pomponi, Shirley A., Reed, John K.
Date Issued: 2007
PURL: http://purl.flvc.org/FCLA/DT/3333011
Subject Headings: Sponges, Macrolides, Marine natural products
Format: Document (PDF)

Title: Isolation and characterization of niphatevirin, a human-immunodeficiency-virus-inhibitory glycoprotein from the marine sponge Niphates erecta.
Creator: O'Keefe, Barry R., Beutler, John A., Cardellina, John H., II, Gulakowski, Robert J., Krepps, Benjamin L., McMahon, James B., Sowder, Raymond C., II, Henderson, Louis E., Pannell, Lewis K., Pomponi, Shirley A., Boyd, Michael R.
Date Issued: 1997
PURL: http://purl.flvc.org/FCLA/DT/3174088
Subject Headings: Sponges, Sponges--Anatomy
Format: Document (PDF)

HBOI Faculty Contributions (99)	+ -
HBOI Technical Reports (8)	+ -
FAU Theses and Dissertations (5)	+ -
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FAU College Collections (1)	+ -

Sponges (48)	+ -
Marine natural products (28)	+ -
Bioactive compounds (12)	+ -
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article (97)	+ -
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Pomponi, Shirley A. (115)	+ -
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