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Title: Effects of a severe winter on invasive green iguanas (Iguana iguana).
Creator: Campbell, Ashley B., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: The green iguana (Iguana iguana) is an herbivorous lizard native to South America and is invasive in south Florida. The severe weather in January 2010 negatively impacted many species and significantly reduced the green iguana population, specifically adult females and juveniles of both sexes. Fifteen adult iguanas from two locations in Palm Beach County were tagged and had blood drawn for blood chemistry and HPLC steroid hormone analysis. Individuals were tracked for home range/territory...
Show moreThe green iguana (Iguana iguana) is an herbivorous lizard native to South America and is invasive in south Florida. The severe weather in January 2010 negatively impacted many species and significantly reduced the green iguana population, specifically adult females and juveniles of both sexes. Fifteen adult iguanas from two locations in Palm Beach County were tagged and had blood drawn for blood chemistry and HPLC steroid hormone analysis. Individuals were tracked for home range/territory analysis and behavioral observations. Blood chemistry values of cold-stunned individuals showed abnormal values similar to those reported in cold-stunned sea turtles. Territoriality and breeding behaviors, including nesting and head-bob displays, decreased or ceased during the following months. Steroid hormone concentrations were detected by HPLC and were not consistent with results from RIA studies in the literature.
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Date Issued: 2011
PURL: http://purl.flvc.org/FAU/3174507
Subject Headings: Weather, Environmental aspects, Adaptation (Biology), Climate changes, Environmental aspects, Conservation biology
Format: Document (PDF)

Title: Anticarcinogenic effects of genistein and anthocyanin extract in MCF-7 human breast cancer cells.
Creator: Stinson, Corine M., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: This study investigated potential apoptotic and anti-proliferative effects of the phytochemicals, genistein and anthocyanin extract, as single and combined treatments in MCF-7 human breast cancer cells. Cells were exposed to single and combined treatments with the phytochemiclas for 48 and 72 hours. Cell viability was assessed using the MTT bioassay. Apoptosis induction was assessed using acridine orange ethidium bromide and rhodamine 123 ethidium bromide fluorescence assays. Both singe and...
Show moreThis study investigated potential apoptotic and anti-proliferative effects of the phytochemicals, genistein and anthocyanin extract, as single and combined treatments in MCF-7 human breast cancer cells. Cells were exposed to single and combined treatments with the phytochemiclas for 48 and 72 hours. Cell viability was assessed using the MTT bioassay. Apoptosis induction was assessed using acridine orange ethidium bromide and rhodamine 123 ethidium bromide fluorescence assays. Both singe and combination treatments induced dose- and time-dependent apoptotic cell death in MCF-7 cells. The percentage of apoptosis was higher in combination treatments than single treatments with either phytochemical, although the difference was not statistically significant. The combination of genistein and anthocyanin extract peaked in efficacy at 48 hours of treatment, to exhibit significantly greater (P<. O5) dose- and time-dependent cell cytotoxicity than single treatments. This study reveals potential chemopreventive implications for the complementary effects of genistein and anthocyanin extract.
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Date Issued: 2011
PURL: http://purl.flvc.org/FAU/3320108
Subject Headings: Phytochemicals, Therapeutic use, Phytoestrogens, Physiological effect, Breast, Cancer, Risk factors, Breast, Cancer, Treatment, Probiotics, Cancer, Chemoprevention, Antioxidants, Therapeutic use
Format: Document (PDF)

Title: Anatomy and physiology of the elasmobranch olfactory system.
Creator: Meredith, Tricia L., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: The olfactory system is the most highly developed system for molecular sensing in vertebrates. Despite their reputation for being particularly olfactory driven, little is known about how this sense functions in elasmobranch fishes. The goal of this dissertation was to examine the morphology and physiology of elasmobranchs to compare their olfactory system with teleost fishes and more derived vertebrates. To test the hypotheses that elasmobranchs possess greater olfactory sensitivities than...
Show moreThe olfactory system is the most highly developed system for molecular sensing in vertebrates. Despite their reputation for being particularly olfactory driven, little is known about how this sense functions in elasmobranch fishes. The goal of this dissertation was to examine the morphology and physiology of elasmobranchs to compare their olfactory system with teleost fishes and more derived vertebrates. To test the hypotheses that elasmobranchs possess greater olfactory sensitivities than teleosts and that lamellar surface area is correlated to sensitivity, I compared the surface area of the olfactory lamellae and the olfactory sensitivities of five phylogenetically diverse elasmobranch species. The olfactory thresholds reported here (10-9 to 10-6 M) were comparable to those previously reported for teleosts and did not correlate with lamellar surface area. Since aquatic species are subject to similar environmental amino acid levels, they appear to have converged upon similar amino acid sensitivities. To test the hypothesis that elasmobranchs are able to detect bile salt odorants despite lacking ciliated olfactory receptor neurons (ORNs), the type of ORN that mediates bile salt detection in the teleosts, I quantified the olfactory specificity and sensitivity of two elasmobranch species to four, teleost-produced C24 bile salts. Both species responded to all four bile salts, but demonstrated smaller relative responses and less sensitivity compared to teleosts and agnathans. This may indicate that elasmobranchs don't rely on bile salts to detect teleost prey. Also, the olfactory system of elasmobranchs contains molecular olfactory receptors for bile salts independent of those that detect amino acids, similar to teleosts., In some elasmobranch species, each olfactory bulb (OB) is physically partitioned into two hemi-bulbs; however, the functional significance of this morphology is not fully understood. The organization of the OBs in three species with varying OB morphologies was examined to test the hypothesis that the elasmobranch OB is somatotopically arranged. Glomeruli in the OB received projections from ORNs in 3-4 olfactory lamellae situated immediately anterior. These results indicate a somatotopic arrangement of the elasmobranch OB, which may be unique among vertebrate olfactory systems and potentially led to the hemi-OB morphology.
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Date Issued: 2011
PURL: http://purl.flvc.org/FAU/3333058
Subject Headings: Condrichthyes, Sense organs, Condrichthyes, Physiology, Sharks, Physiology, Adaptation (Biology), Aquatic animals, Physiology
Format: Document (PDF)

Title: Biomonitoring of hypoxia and sulfide stress in three sub-tropical seagrasses.
Creator: Irwin, Connor., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Hypoxia and sulfide exposure, increased using glucose, are considered major environmental stressors in seagrass communities. Quantum efficiency, total soluble protein and catalase activity were quantified to evaluate the applicability of each of these bioindicators to detect environmental stress in three tropical seagrass species, Thalassia testudinum (Banks ex Kèoenig), Halodule wrightii (Ascherson) and Syringodium filiforme (Kuetz). Hypoxia + sulfide treatments significantly decreased the...
Show moreHypoxia and sulfide exposure, increased using glucose, are considered major environmental stressors in seagrass communities. Quantum efficiency, total soluble protein and catalase activity were quantified to evaluate the applicability of each of these bioindicators to detect environmental stress in three tropical seagrass species, Thalassia testudinum (Banks ex Kèoenig), Halodule wrightii (Ascherson) and Syringodium filiforme (Kuetz). Hypoxia + sulfide treatments significantly decreased the quantum efficiency of all three species, but showed no response in protein and catalase activity. Although no treatment effect was found, catalase activity was enhanced in T. testudinum leaves and H. wrightii roots relative to other tissues, while S. filiforme showed no location-specific catalase activity. These results indicate that quantum efficiency is a more sensitive indicator than protein and catalase activity to hypoxia and sulfide stress in seagrasses.
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Date Issued: 2010
PURL: http://purl.flvc.org/FAU/2976445
Subject Headings: Plant physiology, Environmental management, Seagrasses, Habitat, Environmental aspects, Sulfites, Physiological effect, Marine ecology
Format: Document (PDF)

Title: Characterization of the MHC II B of the bald eagle.
Creator: Smith, Andrew., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: The Major Histocompatibility Complex class II B (MHC II B) gene encodes a protein that is part of the adaptive immune system and critical for the non-self recognition ability of immune cells. This gene has been characterized in the Bald Eagle, ten unique alleles were found in two subpopulations at the geographic extremes of the range margins. Geographic genetic variation is suggested by the presence of population specific alleles. The results showed considerable divergence of groups of Bald...
Show moreThe Major Histocompatibility Complex class II B (MHC II B) gene encodes a protein that is part of the adaptive immune system and critical for the non-self recognition ability of immune cells. This gene has been characterized in the Bald Eagle, ten unique alleles were found in two subpopulations at the geographic extremes of the range margins. Geographic genetic variation is suggested by the presence of population specific alleles. The results showed considerable divergence of groups of Bald Eagle alleles when compared to alleles from other birds of prey. Particular codons within the exon II show signs of balancing selection driving the evolution of the MHC II B. Transcription data showed statistically significant differential expression of alleles. This can be interpreted as meaning a particular locus is being preferentially expressed in blood. The analysis of the polymorphism of this adaptive marker may aid managers of wildlife during this age of global climate change and the biodiversity crisis.
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Date Issued: 2010
PURL: http://purl.flvc.org/FAU/2979375
Subject Headings: Major histocompatibility complex, Cellular signal transduction, Immunogenetics, Genetic polymorphisms
Format: Document (PDF)

Title: Cleavage of brain glutamic acid decarboxylase 65 by calpain under pathological conditions.
Creator: Buddhala, Chandana, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Brain glutamic acid decarboxylase 65 (GAD65) catalyzes the rate-limiting step in the biosynthesis of the major inhibitory neurotransmitter-amino butyric acid (GABA) from the substrate L-glutamic acid. Severe lapse in GABA neurotransmission is one of the etiologies documented in the manifestation of certain neurodegenerative diseases such as epilepsy, Parkinson's disease, Huntington's disease etc. Because GAD65 synthesizes GABA, any modulation of GAD65, therefore, has direct implications on...
Show moreBrain glutamic acid decarboxylase 65 (GAD65) catalyzes the rate-limiting step in the biosynthesis of the major inhibitory neurotransmitter-amino butyric acid (GABA) from the substrate L-glutamic acid. Severe lapse in GABA neurotransmission is one of the etiologies documented in the manifestation of certain neurodegenerative diseases such as epilepsy, Parkinson's disease, Huntington's disease etc. Because GAD65 synthesizes GABA, any modulation of GAD65, therefore, has direct implications on the quanta of GABA released at the synapse. Hence, the major objective of this study was to focus on the regulation of GAD65, with special emphasis on investigating the proteolytic cleavage of fGAD65. Previously, we have shown in vitro that GAD65 was cleaved to form its truncated form (tGAD65), which was more active than the full length form (fGAD65). The enzyme responsible for cleavage was later identified as calpain. Calpain is known to cleave its substrates either under a transient physiologica l stimulus or upon a sustained pathological insult. However, the precise role of calpain cleavage of fGAD65 is poorly understood. In this study, we examined the cleavage of fGAD65 under a range of conditions encompassing both physiological and pathological aspects, including rats under ischemia/reperfusion insult, rat brain synaptosomes or primary neuronal cultures subjected to excitotoxic stimulation with KCl. It was observed that the formation of tGAD65 progressively increased with increasing stimulus concentration. More importantly, cleavage of synaptic vesicle (SV) - associated fGAD65 by calpain was demonstrated, and the resulting tGAD65 harboring the active site of the enzyme was detached from the SVs. Vesicular uptake of the newly synthesized GABA into the SVs was found to be reduced in calpain treated SVs. Furthermore, we also observed that the levels of tGAD65 in the focal cerebral ischemic rat brain tissue increased corresponding to the elevation of local glutamate indica, d by in vivo micro dialysis. Based on these observations, we conclude that calpain cleavage of fGAD65 occurs under pathological conditions.
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Date Issued: 2012
PURL: http://purl.flvc.org/FAU/3342053
Subject Headings: Glutamic acids, Antagonists, Proteolytic enzymes, Research, Cellular signal transduction, Calpain, Glutamic acid, Metabolism
Format: Document (PDF)

Title: Comprehensive study of the ZAD family of zinc finger transcription factors in Drosophila melanogaster.
Creator: Krystel, Joseph., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: The zinc finger associated domain (ZAD) family of transcription factors from Drosophila melanogaster is not well described in the literature, in part because it is very difficult to study by traditional mutagenesis screens. Bioinformatic studies indicate this is due to overlapping functions remaining after a recent evolutionary divergence. I set out to use in vitro-binding techniques to identify the characteristics of the ZAD family and test this theory. I have constructed glutathione S...
Show moreThe zinc finger associated domain (ZAD) family of transcription factors from Drosophila melanogaster is not well described in the literature, in part because it is very difficult to study by traditional mutagenesis screens. Bioinformatic studies indicate this is due to overlapping functions remaining after a recent evolutionary divergence. I set out to use in vitro-binding techniques to identify the characteristics of the ZAD family and test this theory. I have constructed glutathione S-transferase (GST)-ZAD domain chimeric proteins for use in pull down protein binding assays,and GST-Zinc finger (ZnF) array domain chimera for electrophoretic mobility shift assays (EMSA). Protein binding assays indicated two putative conserved interactors, similar to the analogous KRAB system in mammals. ... Competitive bindings were carried out to show a specificity of binding conferred by the identified conserved positions. While the consensus binding sites show relatively few similarities, the predicted target genes identified by the consensus binding sites show significant overlap. The nature of this overlap conforms to the known characteristics of the ZAD family but points to a more positive selection to maintain conservation of function.
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Date Issued: 2012
PURL: http://purl.flvc.org/FAU/3355627
Subject Headings: Cellular signal transduction, Drosophila melanogaster, Cytogenetics, Transcription factors, Zinc-finger proteins, Synthesis, Genetic transcription, Regulation, Gene expression
Format: Document (PDF)

Title: Consumptive effects of predatory fish reduce wetland crayfish (Procambarus spp.) recruitment and drive species turnover.
Creator: Kellogg, Christopher M., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Trade-offs in traits conferring success in permanent and ephemeral habitats are often at odds with few species being able to persist in both types of environments. I examined the effect of sunfish predators on two species of south Florida crayfish to establish the mechanism that limits one species, Procambarus alleni, to short-hydroperiod environments. The crayfish assemblage response to a gradient of sunfish predators and the effect of predation on P. fallax alone was examined. I also...
Show moreTrade-offs in traits conferring success in permanent and ephemeral habitats are often at odds with few species being able to persist in both types of environments. I examined the effect of sunfish predators on two species of south Florida crayfish to establish the mechanism that limits one species, Procambarus alleni, to short-hydroperiod environments. The crayfish assemblage response to a gradient of sunfish predators and the effect of predation on P. fallax alone was examined. I also examined the effects of sunfish on crayfish growth and quantified activity levels and risky behaviors of both crayfish species. P. alleni dominated at low sunfish densities but dominance shifted with increasing sunfish density. P. alleni was more active and likely to initiate risky behaviors, suggesting that sunfish predators remove the more active P. alleni, reducing their numbers disproportionally to those of P. fallax and allowing P. fallax to dominate crayfish assemblages in long-hydroperiod wetlands.
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Date Issued: 2011
PURL: http://purl.flvc.org/FAU/3171400
Subject Headings: Wetland ecology, Habitat selection, Marine ecosystem management, Predatory marine animals, Ecology
Format: Document (PDF)

Title: Dietary niche relationships of white ibis, tricolored heron and snowy egret nestlings in the northern Everglades.
Creator: Boyle, Robin A., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Food availability is the primary factor affecting the reproductive success in many species of birds. Diet composition can indicate diet quality, habitat use and niche requirements for breeding birds and may be variable across short and long-term time scales. Identifying primary prey types of nesting wading birds is important for the hydrologic restoration of wetlands. I collected nestling boluses during the 2008 and 2009 nesting seasons from three species of wading birds that nest in the...
Show moreFood availability is the primary factor affecting the reproductive success in many species of birds. Diet composition can indicate diet quality, habitat use and niche requirements for breeding birds and may be variable across short and long-term time scales. Identifying primary prey types of nesting wading birds is important for the hydrologic restoration of wetlands. I collected nestling boluses during the 2008 and 2009 nesting seasons from three species of wading birds that nest in the northern Everglades: White Ibis, Tricolored Herons and Snowy Egrets. White Ibis bolus composition was dominated by crayfish in both years, but exhibited some variation with landscape water depth in 2009; fish use was greatest when the wetland landscape was relatively dry. In contrast, the prey of Tricolored Herons and Snowy Egrets were primarily fish and their respective diets did not differ from one another in either fish species composition or size structure.
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Date Issued: 2010
PURL: http://purl.flvc.org/FAU/2953207
Subject Headings: Wetland ecology, Bird populations, Water birds
Format: Document (PDF)

Title: Elucidation of the features of the zinc finger associated domain (ZAD) family of transportation factors in Drosophila melanogaster.
Creator: Krystel, Joseph., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: The zinc finger associated domain (ZAD) containing family of transcription factors is not well described in the literature, in part because it is very difficult to study by mutagenesis. We used in vitro-binding techniques to identify characteristics of the ZAD family, by constructing glutathione Stransferase (GST)-ZAD domain chimeric proteins for use in protein binding assays, and GST-Zinc finger array domain chimera for binding site selections. Protein binding assays indicated a possible...
Show moreThe zinc finger associated domain (ZAD) containing family of transcription factors is not well described in the literature, in part because it is very difficult to study by mutagenesis. We used in vitro-binding techniques to identify characteristics of the ZAD family, by constructing glutathione Stransferase (GST)-ZAD domain chimeric proteins for use in protein binding assays, and GST-Zinc finger array domain chimera for binding site selections. Protein binding assays indicated a possible shared cofactor, as seen in the analogous KRAB system in mammals. DNA binding assays have provided a consensus binding sequence for five of the ZAD proteins, consistent with previously reported work on ZAD and unpublished work on mammalian transcription factors. Research is ongoing with an additional ~50 ZAD proteins to more fully map the binding characters of ZAD proteins.
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Date Issued: 2009
PURL: http://purl.flvc.org/FAU/186768
Subject Headings: Cellular signal transduction, Drosophila melanogaster, Cytogenetics, Transcription factors, Zinc-finger proteins, Synthesis, Genetic transcription, Regulation, Gene expression
Format: Document (PDF)

Title: Electroreception in the obligate freshwater stingray, Potamotrygon motoro.
Creator: Harris, Lindsay L., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Elasmobranch fishes use electroreception to detect electric fields in the environment, particularly minute bioelectric fields produced by potential prey. A single elasmobranch family (Potamotrygonidae) is composed of obligate freshwater stingrays endemic to the Amazon River. A freshwater existence has imposed morphological adaptions on their electrosensory system due to life in a high impedance medium. Because their electrosensory morphology differs from their marine relatives, freshwater...
Show moreElasmobranch fishes use electroreception to detect electric fields in the environment, particularly minute bioelectric fields produced by potential prey. A single elasmobranch family (Potamotrygonidae) is composed of obligate freshwater stingrays endemic to the Amazon River. A freshwater existence has imposed morphological adaptions on their electrosensory system due to life in a high impedance medium. Because their electrosensory morphology differs from their marine relatives, freshwater stingrays may demonstrate corresponding differences in behavioral sensitivity. The objective of this study was to quantify behavioral sensitivity of the obligate freshwater stingray Potamotrygon motoro to prey-simulating voltage. The voltage produced by common teleost prey of P. motoro were measured and replicated for behavioral trials. The best response was 10.62 cm, and the smallest voltage gradient detected was 0.005 mVcm-1. This sensitivity is reduced compared to marine species. The conductivity of the medium, more so than ampullary morphology, may dictate sensitivity of the elasmobranch electrosensory system.
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Date Issued: 2013
PURL: http://purl.flvc.org/fcla/dt/3362382
Subject Headings: Fishes, Sense organs, FIshes, Physiology, Stingrays, Physiology, Fish culture
Format: Document (PDF)

Title: Electrogenic metals for elasmobranch bycatch mitigation.
Creator: Smith, Kieran Thomas., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Commercial longline fishing results in large amounts of incidental bycatch of elasmobranch fishes (sharks, skates, and rays). Teleost species lack electrosensory systems and development of technologies which target the ampullary organs of sharks provides an avenue to selectively deter elasmobranchs without affecting the catch rate of target teleosts. Electric field measurements and a controlled scientific longline study were conducted testing whether the lanthanide metal neodymium or zinc...
Show moreCommercial longline fishing results in large amounts of incidental bycatch of elasmobranch fishes (sharks, skates, and rays). Teleost species lack electrosensory systems and development of technologies which target the ampullary organs of sharks provides an avenue to selectively deter elasmobranchs without affecting the catch rate of target teleosts. Electric field measurements and a controlled scientific longline study were conducted testing whether the lanthanide metal neodymium or zinc/graphite might reduce elasmobranch catch per unit effort (CPUE). Baited longline hooks were treated with neodymium and zinc/graphite and catch rates were compared to that of controls. Shark CPUE decreased by 60% on neodymium treated hooks and 80% on zinc/graphite treated hooks. The effectiveness of both treatments varied among species with significant reductions shown for Atlantic sharpnose sharks (Rhizoprionodon terranovae) but less dramatic differences for others. Zinc/graphite is potentially a viable tool for reduction of shark bycatch in a commercial longline fishery.
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Date Issued: 2013
PURL: http://purl.flvc.org/fcla/dt/3361053
Subject Headings: Fishery conservation, Sharks, Conservation, Lanthanide shift reagents, Wildlife management
Format: Document (PDF)

Title: Development of a novel assay for in vivo screening of neuromodulatory drugs and targeted disruption of cholinergic synaptic transmission in Drosophila melanogaster.
Creator: Mejia, Monica, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Finding novel compounds that affect neuronal or muscular function is of great interest, as they can serve as potential pharmacological agents for a variety of neurological disorders. For instance, conopeptides have been developed into powerful drugs like the painkiller PrialtTM. Most conopeptides, however, have yet to be characterized, revealing the need for a rapid and straightforward screening method. We have designed a novel bioassay, which allows for unbiased screening of biological...
Show moreFinding novel compounds that affect neuronal or muscular function is of great interest, as they can serve as potential pharmacological agents for a variety of neurological disorders. For instance, conopeptides have been developed into powerful drugs like the painkiller PrialtTM. Most conopeptides, however, have yet to be characterized, revealing the need for a rapid and straightforward screening method. We have designed a novel bioassay, which allows for unbiased screening of biological activity of compounds in vivo against numerous molecular targets on a wide variety of neurons and muscles in a rapid and straightforward manner. For this, we paired nanoinjection of compounds with electrophysiological recordings from the Giant Fiber System of Drosophila melanogaster, which mediates the escape response of the fly.
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Date Issued: 2013
PURL: http://purl.flvc.org/fcla/dt/3362560
Subject Headings: Drosophila melanogaster, Genetics, Drosophila melanogaster, Life cycles, Insects, Physiology, Developmental neurobiology, Neural transmission, Cholinergic mechanisms
Format: Document (PDF)

Title: Does size really matter: how synchrony and size affect the dynamic of aggression between two sympatric species of dolphin in the Bahamas.
Creator: Cuksick, Jessica A., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Bottlenose (Tursiops truncatus) and spotted (Stenella frontalis) dolphins are sympatric species, resident to Little Bahama Bank, Bahamas. A unique, dynamic methodology quantified how interspecific aggression changed over time in terms of the individuals participating, context, and behaviors used. The timing of human observation relative to the onset of aggression did not result in differences in the proportion of behaviors observed. Highly intense behaviors were used most often. The...
Show moreBottlenose (Tursiops truncatus) and spotted (Stenella frontalis) dolphins are sympatric species, resident to Little Bahama Bank, Bahamas. A unique, dynamic methodology quantified how interspecific aggression changed over time in terms of the individuals participating, context, and behaviors used. The timing of human observation relative to the onset of aggression did not result in differences in the proportion of behaviors observed. Highly intense behaviors were used most often. The synchronous state of spotted dolphin groups, not the presence alone, was a crucial factor in determining the onset and progression of aggression. When synchronous, spotted dolphins successfully dominated the larger bottlenose dolphins. Two levels of dominance were observed. Within a single encounter ("encounter level"), one species did dominate the other. When all aggressive encounters were considered collectively over the long term ("gross level"), one species did not dominate the other. The combination of contextual factors best determined the dynamic of interspecific aggression.
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Date Issued: 2012
PURL: http://purl.flvc.org/FAU/3342044
Subject Headings: Bottlenose dolphin, Behavior, Research, Atlantic spotted dolphin, Behavior, Research, Mammals, Behavior, Aggressive behavior in animals
Format: Document (PDF)

Title: Diversity and selection in the major histocompatibility complex: DQA and immune function in IRL and Atlantic bottlenose dolphins (Tursiops truncatus).
Creator: Ferrer, Tatiana., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: The Major Histocompatibility Complex (MHC) encodes proteins critical to the vertebrate immune response; therefore MHC diversity is an indicator of population health. I have (1) Isolated exon 2 of the class II gene DQA in Tursiops truncatus in the North Indian River Lagoon (IRL) (n=17), South IRL (n=29) and adjacent Atlantic waters (n=20), (2) assessed genetic variability between groups, (3) developed a method to genotype individuals, (4) typed 11 unique alleles in 66 individuals, (5) detected...
Show moreThe Major Histocompatibility Complex (MHC) encodes proteins critical to the vertebrate immune response; therefore MHC diversity is an indicator of population health. I have (1) Isolated exon 2 of the class II gene DQA in Tursiops truncatus in the North Indian River Lagoon (IRL) (n=17), South IRL (n=29) and adjacent Atlantic waters (n=20), (2) assessed genetic variability between groups, (3) developed a method to genotype individuals, (4) typed 11 unique alleles in 66 individuals, (5) detected geographic patterns of diversity between estuarine and coastal individuals (FST=0.1255, p<0.05), (6) found evidence of positive selection centered in the binding pockets P1, P6 and P9 of the peptide binding region (w=2.08), (7) found that patterns of polymorphism did not closely match patterns of diversity in neutral markers, (8) performed a pilot study with Orcinus orca. The initial findings highlight the need for further comparative work and suggest that silent mutations are not neutral.
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Date Issued: 2013
PURL: http://purl.flvc.org/fcla/dt/3362335
Subject Headings: Major histocompatibility complex, Immunogenetics, Molecular genetics, Endocrine disrupting chemicals, Dolphins, Geographical distribution, Population genetics, Social behavior in animals
Format: Document (PDF)

Title: Function of glycinergic interplexiform cells in rod synaptic transmission.
Creator: Jiang, Zheng., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: The interplexiform cells(IP cells) are the most recently discovered neurons in the retina and their function is to provide centrifugal feedback in retina. The anatomical structure of the IP cells has been well studied, but the function of these neurons is largely unknown. I systematically studied the excitatory and inhibitory inputs from IP cells in salamander retina. I found that L-EPSCs in IP cells are mediated by AMPA and NMDA receptors; in addition, L-IPSCs are mediated by glycine...
Show moreThe interplexiform cells(IP cells) are the most recently discovered neurons in the retina and their function is to provide centrifugal feedback in retina. The anatomical structure of the IP cells has been well studied, but the function of these neurons is largely unknown. I systematically studied the excitatory and inhibitory inputs from IP cells in salamander retina. I found that L-EPSCs in IP cells are mediated by AMPA and NMDA receptors; in addition, L-IPSCs are mediated by glycine receptors and GABAC receptors. In response to light, IP cells reaction potentials transiently at the onset and onset of light stimulation. The major neural transmitter of IP cells in salamander retina is glycine. We also studied the distribution and function of glycine transporters. Our result indicates that GlyT1- and GlyT2-like transporters were present in Muller cells and neurons. The glycine feedback at outer plexiform layer (OPL) has effects on both the bipolar cell dendrites and rod photoreceptor terminals. At bipolar cell dendrites, glycine selectively depolarizes rod-dominant On-bipolar cells, and hyperpolarizes Off- bipolar cells. At rod photoreceptor terminals, 10 M glycine activates voltage-gated Ca2+ channels. These effects facilitated glutamate vesicle release in photoreceptors. It increases the sEPSC in OFF bipolar cells. The combined effect of glycine at rod terminals and bipolar cell dendrites leads to enhanced dim light signal transduction in the rod photoreceptor to ganglion cell pathway. This study provides a model that displays the function of centrifugal feedback through IP cells in the retina.
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Date Issued: 2009
PURL: http://purl.flvc.org/FAU/2708369
Subject Headings: Cellular signal transduction, Neurochemistry, Neuroplasticity, Synapses, Retina, Cytology, Visual pathways
Format: Document (PDF)

Title: Effects of stressors on differential gene expression and secondary metabolites by Axinella corrugata.
Creator: Grima, Jennifer., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Sponges are an important source of bioactive marine natural products, or secondary metabolites. The common Caribbean reef sponge, Axinella corrugata, produces an antitumor and antibacterial chemical, stevensine. This study determined whether environmental stressors, such as elevated temperature and exposure to Amphibalanus amphitrite larvae, affect the production of stevensine by A.corrugata and if the stressors caused A.corrugata to exhibit differential gene expression. Temperature stress...
Show moreSponges are an important source of bioactive marine natural products, or secondary metabolites. The common Caribbean reef sponge, Axinella corrugata, produces an antitumor and antibacterial chemical, stevensine. This study determined whether environmental stressors, such as elevated temperature and exposure to Amphibalanus amphitrite larvae, affect the production of stevensine by A.corrugata and if the stressors caused A.corrugata to exhibit differential gene expression. Temperature stress resulted in no significant change in the production of stevensine; only two genes were significantly differentially expressed, including hsp70. Larval stressed resulted in increased production of stevensine and significant differential gene expression (more than seventy genes). This study suggests that A.corrugata may be resilient to elevations in temperature and that one of stevensine's roles in nature is as an antifoulant.
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Date Issued: 2013
PURL: http://purl.flvc.org/fcla/dt/3360781
Subject Headings: Axinellida, Sponges, Marine pharmacology, Adaptation (Biology), Gene expression, Genetic regulation, Stress (Physiology), Ecophysiology
Format: Document (PDF)

Title: Engineered and natural TIMP mutations.
Creator: Hamze, Asmaa Bilal., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Tissue inhibitors of metalloproteinases (TIMPs) comprise a family of four proteins in humans that modulate the turnover of the extracellular matrix by regulating the activities of endopeptidases that catalyze its degradation, especially the matrix metalloproteinases (MMP). In general, the four TIMPs are broad-spectrum tight binding inhibitors of MMPs with individual differences in specificity. In this study, we attempted to understand the basis of such variation by using membrane type-1 MMP ...
Show moreTissue inhibitors of metalloproteinases (TIMPs) comprise a family of four proteins in humans that modulate the turnover of the extracellular matrix by regulating the activities of endopeptidases that catalyze its degradation, especially the matrix metalloproteinases (MMP). In general, the four TIMPs are broad-spectrum tight binding inhibitors of MMPs with individual differences in specificity. In this study, we attempted to understand the basis of such variation by using membrane type-1 MMP (MT1-MMP) as a model, since it is inefficiently inhibited by TIMP-1 in contrast with the other TIMPs. We designed and engineered mutations in the N-domain of TIMP-1, based on current knowledge of TIMP interactions. By measuring inhibition levels of each mutant against several MMPs, including MT1-MMP, we were able to obtain a triple mutant with an vii improved affinity for MT1-MMP., Our results, along with previous data, confirm that multiple residues in the critical interface segments between TIMPs and MMPs, namely at positions 2, 4, 5, 6, and 98, are key in determining the basic interaction between the two molecules. The second part of this work focused on naturally occurring mutations in TIMP-3 which cause an early form of macular degeneration called Sorsby's Fundus Dystrophy (SFD). The TIMP-3 mutants identified so far share certain features but the mechanism by which they result in macular disease is not yet understood. As an initial step, we expressed recombinant TIMP-3 carrying a truncation mutation, glutamic acid 139 to a stop codon (E139X), and assessed its activity towards representative MMPs and tumor necrosis factor-(Sa (Bconverting enzyme, another metalloproteinase normally inhibited by TIMP-3. Our results indicate that this mutation does not impair the inhibitory activity of TIMP-3., Expression of this mutant in mammalian retinal cells revealed a difference in localization between wild-type and E139X mutant TIMP-3. Therefore, we concluded that the SFD mutations may actually influence the processing and/or binding properties of TIMP-3 in the retina.
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Date Issued: 2008
PURL: http://purl.flvc.org/FAU/186296
Subject Headings: Proteolytic enzymes, Extracellular matrix proteins, Metalloproteinases, Inhibitors, Apoptosis, Retinal degeneration, Research
Format: Document (PDF)

Title: Effects of mid-incubation egg movement on loggerhead (Caretta caretta) turtle hatch success and embryo development.
Creator: Ahles, Natasha M., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Due to an emergency status dune restoration project following Subtropical Storm Andrea in 2007 on Singer Island, Florida, nests needed to be moved during early to mid-incubation. Nesting success was compared between those moved mid-incubation, moved within 12h to either a native sand incubation area or a renourished sand incubation area, and those left in-situ. Nests moved within 12h to the native sand had a significantly larger proportion of pipped hatchlings. Nests moved mid-incubation had...
Show moreDue to an emergency status dune restoration project following Subtropical Storm Andrea in 2007 on Singer Island, Florida, nests needed to be moved during early to mid-incubation. Nesting success was compared between those moved mid-incubation, moved within 12h to either a native sand incubation area or a renourished sand incubation area, and those left in-situ. Nests moved within 12h to the native sand had a significantly larger proportion of pipped hatchlings. Nests moved mid-incubation had a significantly lower proportion of hatched eggs as well as emergent hatchlings. The stage in which embryonic development was arrested corresponded to the stage the embryos were in during the time of movement; indicating movement was the cause of death. When comparing nests moved within the initial 2.5 weeks of development to those moved after 2.5 weeks of development, there was no significant difference in hatching success.
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Date Issued: 2009
PURL: http://purl.flvc.org/FAU/221948
Subject Headings: Sea turtles, Embryology, Wildlife conservation
Format: Document (PDF)

Title: Activators and repressors of transcription: using bioinformatics approaches to analyze and group human transcription factors.
Creator: Savitskaya, Ala., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Transcription factors are macromolecules that are involved in transcriptional regulation by interacting with specific DNA regions, and they can cause activation or silencing of their target genes. Gene regulation by transcriptional control explains different biological processes such as development, function, and disease. Even though transcriptional control has been of great interest for molecular biology, much still remains unknown. This study was designed to generate the most current list...
Show moreTranscription factors are macromolecules that are involved in transcriptional regulation by interacting with specific DNA regions, and they can cause activation or silencing of their target genes. Gene regulation by transcriptional control explains different biological processes such as development, function, and disease. Even though transcriptional control has been of great interest for molecular biology, much still remains unknown. This study was designed to generate the most current list of human transcription factor genes. Unique entries of transcription factor genes were collected and entered into Microsoft Office 2007 Access Database along with information about each gene. Microsoft Office 2007 Access tools were used to analyze and group collected entries according to different properties such as activator or repressor record, or presence of certain protein domains. Furthermore, protein sequence alignments of members of different groups were performed, and phylogenetic trees were used to analyze relationship between different members of each group. This work contributes to the existing knowledge of transcriptional regulation in humans.
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Date Issued: 2010
PURL: http://purl.flvc.org/FAU/1930495
Subject Headings: Transcription factors, Genetic transcription, Regulation, Cellular signal transduction, DNA microarrays, Bioinformatics
Format: Document (PDF)

Cellular signal transduction (31)	+ -
Predation (Biology) (30)	+ -
Drosophila melanogaster (17)	+ -
Adaptation (Biology) (16)	+ -
Wetland ecology (14)	+ -

Animal behavior (13)	+ -
Ecology (13)	+ -
Sea turtles (13)	+ -
Gene expression (12)	+ -
Genetic transcription (12)	+ -
Loggerhead turtle (11)	+ -
Physiology (11)	+ -
Marine ecology (10)	+ -
Wildlife conservation (10)	+ -
Habitat (9)	+ -
Physiological effect (9)	+ -
Regulation (9)	+ -
Cellular signal transduction. (8)	+ -
Molecular aspects (8)	+ -
Population genetics (8)	+ -
Proteins (8)	+ -
Social behavior in animals (8)	+ -
Synthesis (8)	+ -
Transcription factors (8)	+ -
Behavior (7)	+ -
Biochemical markers (7)	+ -
Chemical modification (7)	+ -
Habitat (Ecology) (7)	+ -
Montastraea (7)	+ -
Mutation (Biology) (7)	+ -

Florida (21)	+ -
Everglades (7)	+ -
Bahamas (5)	+ -
Atlantic Ocean (4)	+ -
Little Bahama Bank (3)	+ -

United States (3)	+ -
Indian River (Fla. : Lagoon) (2)	+ -
Indian River (Lagoon) (2)	+ -
Abandoned farms--Florida--Everglades National Park (1)	+ -
Atlantic Coast (U.S.) (1)	+ -
Behavior (1)	+ -
Canaveral National Seashore (Fla.) (1)	+ -
Coral reef ecology--Florida (1)	+ -
Everglades Region (1)	+ -
Fishes--Florida (1)	+ -
Florida Keys (1)	+ -
Indian River Lagoon Estuary System (1)	+ -
Lake Okeechobee (1)	+ -
Mexico, Gulf of (1)	+ -
Okeechobee, Lake (Fla.) (1)	+ -
Plant succession--Florida--Everglades National Park (1)	+ -
Seminole Indians--Florida (1)	+ -
West Palm Beach (1)	+ -
Wildlife conservation--Florida (1)	+ -
Wildlife management--Florida (1)	+ -

Charles E. Schmidt College of Science (410)	+ -
Florida Atlantic University (305)	+ -
Salmon, Michael (15)	+ -
Gawlik, Dale E. (14)	+ -
Milton, Sarah L. (14)	+ -

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