Current Search: Charles E. Schmidt College of Science (x)
View All Items
Pages
- Title
- Regulation of growth by TGF-B in Drosophila.
- Creator
- Gesualdi, Scott C., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Key to our understanding of growth regulation in Drosophila would be discovering a ligand that could regulate steroid synthesis. Activins are involved in regulating steroid hormone release in vertebrates. In invertebrates, they most likely function to keep ecdysone levels low to allow the larvae more time to achieve critical weight in order to initiate the metamorphic process. TGF-B(Transforming Growth Factor Beta) is a family of cytokine growth factors. We find that two members of the TGF-B...
Show moreKey to our understanding of growth regulation in Drosophila would be discovering a ligand that could regulate steroid synthesis. Activins are involved in regulating steroid hormone release in vertebrates. In invertebrates, they most likely function to keep ecdysone levels low to allow the larvae more time to achieve critical weight in order to initiate the metamorphic process. TGF-B(Transforming Growth Factor Beta) is a family of cytokine growth factors. We find that two members of the TGF-B signaling pathway Drosophila Activin (dACT) and Activin-like ligand Dawdle (DAW) signal through the type I receptor Baboon (BABO) and the type II receptor PUNT to primarily activate the transcription factor dSMAD2 and MAD to a lesser extent. One transcription factor brinker (brk) appears to be central to dACT signaling., In wings dACT signaling is necessary to promote growth however, dACT is not expressed in wings suggesting that dACT is provided through the endocrine system. One possible target tissue of dACT signaling is the ring gland (RG), which synthesizes and secretes the steroid hormone ecdysone (E). Consistent with this idea, using the UAS/GAL-4 system, we find that over-expression of the TGF-B ligand dACT with the neuroendocrine driver 386Y-GAL4 results in an increase in the size of flies. Surprisingly, when we increase the dose with two copies of dACT, it decreases the size of flies also indicating non-autononomous effects. We find that overexpression of the activated form of the dACT type I receptor Baboon (BABO) or brk with the ring gland specific driver phm-GAL4 results in developmental arrest of larvae that stay small and never pupate. The developmental arrest can be overcome by feeding larvae E, suggesting that dACT represses E through brk. These results suggest a model where dACT signaling activates brk which inhibits E. We picked three cytochrome P450 enzymes: phantom (PHM), disembodied (DIS) and spookier (SPKR)., PHM is not regulated by any component in the dACT signaling pathway however, we find DIS and SPKR are down-regulated through brk. MAD and dSmad2 bind to a Smad binding site and MAD out-competes dSMAD2. We find no evidence that Drosophila insulin-like peptides (DILPS)/PI3- Kinase or Ras signal through the dActivin signaling pathway.
Show less - Date Issued
- 2009
- PURL
- http://purl.flvc.org/FAU/228773
- Subject Headings
- Cell differentiation, Developmental genetics, Integrins, Cellular control mechanisms
- Format
- Document (PDF)
- Title
- RNA oxidative damage and ribosomal RNA surveillance under oxidative stress.
- Creator
- Liu, Min, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
We have studies oxidative damage of RNA, a major type of cellular macromolecules. RNA is a primary target of reactive oxygen species (ROS). Under oxidative stress, most nucleic acid damages in Escherichia coli (E.coli) are present in RNA as shown by high levels of 8-oxo-G, an oxidized form of guanine. Increased RNA oxidation is closely correlated to cell death under oxidative stress. Surprisingly, neither RNA structure nor association with proteins protects RNA from oxidation... Our results...
Show moreWe have studies oxidative damage of RNA, a major type of cellular macromolecules. RNA is a primary target of reactive oxygen species (ROS). Under oxidative stress, most nucleic acid damages in Escherichia coli (E.coli) are present in RNA as shown by high levels of 8-oxo-G, an oxidized form of guanine. Increased RNA oxidation is closely correlated to cell death under oxidative stress. Surprisingly, neither RNA structure nor association with proteins protects RNA from oxidation... Our results demonstrate a major role for RNA degradation in controlling oxidized RNA. We have identified activities that may work in specific pathways for selectively degrading damaged RNA. These activities may play pivotal rold in controlling oxidized RNA and protecting cells under oxidative stress.
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3355620
- Subject Headings
- RNA, Metabolism, Cellular signal transduction, Genetic translation, Molecular biology
- Format
- Document (PDF)
- Title
- Quantifying the energetic cost of disorientation in loggerhead (Caretta caretta) and green (Chelonia mydas) sea turtle hatchlings.
- Creator
- Williams, Sean., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
The photopollution of beaches caused by artificial light sources can interfere with sea turtle hatchlings' ability to orient properly towards the sea. Exhaustion due to extended disorientation crawling may then increase hatchling mortality. This study evaluated the energetic consequences of long-distance crawls in green and loggerhead sea turtle hatchlings. Turtles crawled for 200 m or 500 m on a treadmill, followed by a 30 minute evaluation of swim performance. Metabolic activity was...
Show moreThe photopollution of beaches caused by artificial light sources can interfere with sea turtle hatchlings' ability to orient properly towards the sea. Exhaustion due to extended disorientation crawling may then increase hatchling mortality. This study evaluated the energetic consequences of long-distance crawls in green and loggerhead sea turtle hatchlings. Turtles crawled for 200 m or 500 m on a treadmill, followed by a 30 minute evaluation of swim performance. Metabolic activity was quantified via measures of blood glucose, blood lactate, and oxygen consumption. No significant changes in metabolism resulted from long-distance crawling activity in loggerheads ; however, loggerheads rested for extended periods of time during the crawl trials, often unresponsive to stimuli. This behavior implies that loggerhead hatchlings would be vulnerable to predation or temperature stress due to exhaustion from extended periods of crawling. Further data is required to draw any significant conclusions about long-term disorientatin crawling in green hatchlings.
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3356891
- Subject Headings
- Sea turtles, Nests, Protection, Sea turtles, Life cycles, Animal behavior, Predation (Biology)
- Format
- Document (PDF)
- Title
- Roles of troponin I in heart development and cardiac function.
- Creator
- Du, Jianfeng., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Two major troponin I (TnI) genes, fetal TnI (ssTnI) and adult TnI (cTnI), are expressed in the mammalian heart under the control of a developmentally regulated program. In this study, the up-stream domain (~1,800 bp) of mouse fetal TnI gene has been cloned and characterized. There is a high homology of this region among mouse, rat and human. Transfection assays indicated that conserved GA-rich sequences, CREB and a CCAAT box within the first 300 bp upstream of the transcription start site...
Show moreTwo major troponin I (TnI) genes, fetal TnI (ssTnI) and adult TnI (cTnI), are expressed in the mammalian heart under the control of a developmentally regulated program. In this study, the up-stream domain (~1,800 bp) of mouse fetal TnI gene has been cloned and characterized. There is a high homology of this region among mouse, rat and human. Transfection assays indicated that conserved GA-rich sequences, CREB and a CCAAT box within the first 300 bp upstream of the transcription start site were critical for the gene expression. Electrophoretic mobility shift assays (EMSAs) and chromatin immunoprecipitation (ChIP) assays revealed binding proteins to CREB site in nuclear extracts from myocardial cells. Thyroid hormone (T3) caused a significant inhibitory effect on ssTnI expression in myocardial cells. Cardiac troponin I (cTnI) mutations have been linked to the development of restrictive cardiomyopathy (RCM) in human patients. We modeled one mutation in human cTnI Cv terminus, arginine1 92 histidine (R192H) by cardiac specific expression of the mutated protein (cTnI193His in mouse sequence) in transgenic mice. The main functional alteration detected in cTnI193His mice by ultrasound cardiac imaging examinations was impaired cardiac relaxation manifested by a decreased left ventricular end diastolic dimension (LVEDD) and an increased end diastolic dimension in both atria. Echocardiography revealed a series of changes on the transgenic mice including a reversed E-to-A ratio, increased deceleration time, and prolonged isovolumetric relaxation time. At the age of 12 months, cardiac output in cTnI193His mice was significantly declined, and some transgenic mice showed congestive heart failure. The negative impact of cTnI193His on ventricular contraction and relaxation was further demonstrated in isolated mouse working heart preparations., Dobutamine stimulation increased heart rate in cTnI193His mice but did not improve CO.The cTnI193His mice had a phenotype similar to that in human RCM patients carrying the cTnI mutation. The results demonstrate a critical role of the COOH-terminal domain of cTnI in the diastolic function of cardiac muscle. This mouse model provides us with a tool to further investigate the pathophysiology and the development of RCM.
Show less - Date Issued
- 2008
- PURL
- http://purl.flvc.org/FAU/186287
- Subject Headings
- Mice as laboratory animals, Biochemical markers, Diagnostic use, Heart, Diseases, Molecular diagnosis, Cardiovascular system, Pathophysiology
- Format
- Document (PDF)
- Title
- Role of methionine sulfoxide reductase in thermal-induced spreading depression coma in Drosophila melanogaster.
- Creator
- Schey, Karin., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Drosophila melanogaster encounter periods of increased temperature or decreased oxygen in its native environment. One consequence of these environmental stresses is increased production of reactive oxygen species that damage major molecules within cells. Another consequence is that flies fall into a protective coma where biological functions are minimized to conserve energy expenditures. This biological phenomenon is called spreading depression. The overarching aim of this project is to...
Show moreDrosophila melanogaster encounter periods of increased temperature or decreased oxygen in its native environment. One consequence of these environmental stresses is increased production of reactive oxygen species that damage major molecules within cells. Another consequence is that flies fall into a protective coma where biological functions are minimized to conserve energy expenditures. This biological phenomenon is called spreading depression. The overarching aim of this project is to determine if methionine sulfoxide reductases affect entrance or exit from the protective coma induced by acute thermal stress. The data revealed that complete deficiency of Msr in young flies causes a faster induction of the coma. In both young and old flies, Msr does not affect average recovery time but does affect the pattern of recovery from coma. Entrance into the coma is age dependent with young flies maintaining activity longer than before entering into the coma as compared to old flies.
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3355873
- Subject Headings
- Cellular signal transduction, Proteins, Chemical modification, Spreading cortical depression, Oxidation-reduction reaction, Aging, Molecular aspects, Mutation (Biology)
- Format
- Document (PDF)
- Title
- Revisiting leisure activities and the risk of dementia in the elderly with special focus on dancing.
- Creator
- Stevens, Carrie., Charles E. Schmidt College of Science, Department of Mathematical Sciences
- Abstract/Description
-
Data was provided by researchers of the Einstein Aging Study (EAS) of the Albert Einstein College of Medicine, Yeshiva University whom statistically analyzed data from the Bronx Aging Study cohort, concluding that participation in cognitive leisure activities and one physical activity, dancing, were associated with a reduced risk of dementia [1]. We explore data from a second (the EAS) cohort, utilizing Cox Proportional-Hazards and extended Cox regression [13]. Cognitive leisure activities in...
Show moreData was provided by researchers of the Einstein Aging Study (EAS) of the Albert Einstein College of Medicine, Yeshiva University whom statistically analyzed data from the Bronx Aging Study cohort, concluding that participation in cognitive leisure activities and one physical activity, dancing, were associated with a reduced risk of dementia [1]. We explore data from a second (the EAS) cohort, utilizing Cox Proportional-Hazards and extended Cox regression [13]. Cognitive leisure activities in general, and particularly doing crossword puzzles, reading books, watching television, and emailing are associated with a reduced risk of dementia. Doing aerobics, learning computer programming, babysitting, dancing, jogging singing, and weight training are associated with an increased risk of dementia. Participation in cognitive leisure activities in general, and reading books in particular, remains highly significant even after adjustment for well-known risk factors [14] such as: age, cognitive status, depression, medical illnesses, gender, ethnicity, education and economic status.
Show less - Date Issued
- 2011
- PURL
- http://purl.flvc.org/FAU/3334097
- Subject Headings
- Aging, Psychological aspects, Older people, Health and hygiene, Forecasting, Older people, Mental health, Forecasting, Alzheimer's disease
- Format
- Document (PDF)
- Title
- Rings of integer-valued polynomials and derivatives.
- Creator
- Villanueva, Yuri., Charles E. Schmidt College of Science, Department of Mathematical Sciences
- Abstract/Description
-
For D an integral domain with field of fractions K and E a subset of K, the ring Int (E,D) = {f e K[X]lf (E) C D} of integer-valued polynomials on E has been well studies. In particulare, when E is a finite subset of D, Chapman, Loper, and Smith, as well as Boynton and Klingler, obtained a bound on the number of elements needed to generate a finitely generated ideal of Ing (E, D) in terms of the corresponding bound for D. We obtain analogous results for Int (r) (E, D) - {f e K [X]lf(k) (E) c...
Show moreFor D an integral domain with field of fractions K and E a subset of K, the ring Int (E,D) = {f e K[X]lf (E) C D} of integer-valued polynomials on E has been well studies. In particulare, when E is a finite subset of D, Chapman, Loper, and Smith, as well as Boynton and Klingler, obtained a bound on the number of elements needed to generate a finitely generated ideal of Ing (E, D) in terms of the corresponding bound for D. We obtain analogous results for Int (r) (E, D) - {f e K [X]lf(k) (E) c D for all 0 < k < r} , for finite E and fixed integer r > 1. These results rely on the work of Skolem [23] and Brizolis [7], who found ways to characterize ideals of Int (E, D) from the values of their polynomials at points in D. We obtain similar results for E = D in case D is local, Noetherian, one-dimensional, analytically irreducible, with finite residue field.
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3356899
- Subject Headings
- Rings of integers, Ideals (Algebra), Polynomials, Arithmetic algebraic geometry, Categories (Mathematics), Commutative algebra
- Format
- Document (PDF)
- Title
- Revisiting the methodology and application of Value-at-Risk.
- Creator
- Chung, Kyong., Charles E. Schmidt College of Science, Department of Mathematical Sciences
- Abstract/Description
-
The main objective of this thesis is to simulate, evaluate and discuss three standard methodologies of calculating Value-at-Risk (VaR) : Historical simulation, the Variance-covariance method and Monte Carlo simulations. Historical simulation is the most common nonparametric method. The Variance-covariance and Monte Carlo simulations are widely used parametric methods. This thesis defines the three aforementioned VaR methodologies, and uses each to calculate 1-day VaR for a hypothetical...
Show moreThe main objective of this thesis is to simulate, evaluate and discuss three standard methodologies of calculating Value-at-Risk (VaR) : Historical simulation, the Variance-covariance method and Monte Carlo simulations. Historical simulation is the most common nonparametric method. The Variance-covariance and Monte Carlo simulations are widely used parametric methods. This thesis defines the three aforementioned VaR methodologies, and uses each to calculate 1-day VaR for a hypothetical portfolio through MATLAB simulations. The evaluation of the results shows that historical simulation yields the most reliable 1-day VaR for the hypothetical portfolio under extreme market conditions. Finally, this paper concludes with a suggestion for further studies : a heavy-tail distribution should be used in order to imporve the accuracy of the results for the two parametric methods used in this study.
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3358328
- Subject Headings
- Valuation, Econometric models, Prices, Econometric models, Financial risk management, Mathematical optimization, Finance, Mathematical models
- Format
- Document (PDF)
- Title
- On the Laplacian and fractional Laplacian in exterior domains, and applications to the dissipative quasi-geostrophic equation.
- Creator
- Kosloff, Leonardo., Charles E. Schmidt College of Science, Department of Mathematical Sciences
- Abstract/Description
-
In this work, we develop an extension of the generalized Fourier transform for exterior domains due to T. Ikebe and A. Ramm for all dimensions n>2 to study the Laplacian, and fractional Laplacian operators in such a domain. Using the harmonic extension approach due to L. Caffarelli and L. Silvestre, we can obtain a localized version of the operator, so that it is precisely the square root of the Laplacian as a self-adjoint operator in L2 with DIrichlet boundary conditions. In turn, this...
Show moreIn this work, we develop an extension of the generalized Fourier transform for exterior domains due to T. Ikebe and A. Ramm for all dimensions n>2 to study the Laplacian, and fractional Laplacian operators in such a domain. Using the harmonic extension approach due to L. Caffarelli and L. Silvestre, we can obtain a localized version of the operator, so that it is precisely the square root of the Laplacian as a self-adjoint operator in L2 with DIrichlet boundary conditions. In turn, this allowed us to obtain a maximum principle for solutions of the dissipative two-dimensional quasi-geostrophic equation the exterior domain, which we apply to prove decay results using an adaptation of the Fourier Splitting method of M.E. Schonbek.
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3355570
- Subject Headings
- Fluid dynamics, Data processing, Laplacian matrices, Attractors (Mathematics), Differential equations, Partial
- Format
- Document (PDF)
- Title
- Patterns of inorganic phosphate and carbohydrate allocation in sawgrass (Cladium jamaicense Crantz) and southern cattail (Typha domingensis Pers.) grown at low and high phosphate levels.
- Creator
- Hill, Brian, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
In recent history, C. jamaicense has been displaced by another native monocot, T. domingensis, predominantly resulting from increased phosphorous enrichment in the Everglades. This study aimed to elucidate these two species responses to low and high [Pi] in terms of allocation, photosynthate partitioning and growth. C. jamaicense growth was independent of Pi, while T. domingensis growth increased with [Pi]. Under high [Pi], allocation to younger T. domingensis shoots occurred, while C....
Show moreIn recent history, C. jamaicense has been displaced by another native monocot, T. domingensis, predominantly resulting from increased phosphorous enrichment in the Everglades. This study aimed to elucidate these two species responses to low and high [Pi] in terms of allocation, photosynthate partitioning and growth. C. jamaicense growth was independent of Pi, while T. domingensis growth increased with [Pi]. Under high [Pi], allocation to younger T. domingensis shoots occurred, while C. jamaicense shoots retained more [Pi], while low [Pi] resulted in homogeneous allocation patterns for both species. Additionally, Pi deficiencies induced carbohydrate levels in older shoots of T. domingensis, while [Pi] had no effect on photosynthate partitioning patterns in C. jamaicense. ACP activity was induced by Pi deficiency in all T. domingensis shoots and increased with shoot age, while no effect was observed in C. jamaicense. Results indicate these two species differ in allocation strategies when [Pi] is altered.
Show less - Date Issued
- 2009
- PURL
- http://purl.flvc.org/FAU/238480
- Subject Headings
- Nitrogen-fixing algae, Biogeochemical cycles, Phosphorus, Psychological transport, Soil stabilization, Ecosystem management, Vegetation dynamics
- Format
- Document (PDF)
- Title
- Pelvic fin locomotion in batoids.
- Creator
- Macesic, Laura Jane., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Although most batoids (skates and rays) are benthic, only the skates (Rajidae) have been described as performing benthic locomotion, termed 'punting'. While keeping the rest of the body motionless, the skate's specialized pelvic fins are planted into the substrate and then retracted caudally, which thrusts the body forward. This may be advantageous for locating and feeding on prey, avoiding predators, and reducing energetic costs. By integrating kinematic, musculoskeletal, material properties...
Show moreAlthough most batoids (skates and rays) are benthic, only the skates (Rajidae) have been described as performing benthic locomotion, termed 'punting'. While keeping the rest of the body motionless, the skate's specialized pelvic fins are planted into the substrate and then retracted caudally, which thrusts the body forward. This may be advantageous for locating and feeding on prey, avoiding predators, and reducing energetic costs. By integrating kinematic, musculoskeletal, material properties, and compositional analyses across a range of morphologically and phylogenetically diverse batoids, this dissertation (i) demonstrates that punting is not confined to the skates, and (ii) provides reliable anatomical and mechanical predictors of punting ability. Batoids in this study performed true punting (employing only pelvic fins), or augmented punting (employing pectoral and pelvic fins). Despite the additional thrust from the pectoral fins, augmented punters failed to exceed the punting c apabilities of the true punters. True punters' pelvic fins had greater surface area and more specialized and robust musculature compared to the augmented punters' fins. The flexural stiffness of the main skeletal element used in punting, the propterygium, correlated with punting ability (3.37 x 10-5 - 1.80 x 10-4 Nm2). Variation was due to differences in mineral content (24.4-48-9% dry mass), and thus, material stiffness (140-2533 MPa), and second moment of area. The propterygium's radius-to-thickness ratio (mean = 5.52 +-0.441 SE) indicated that the propterygium would support true and augmented punters, but not non-punters, in an aquatic environment. All propterygia would fail on land. Geometric and linear morphometric analyses of 61 batoid pelvic girdles demonstrated that pelvic girdle shape can predict punting and swimming ability and taxonomic attribution to Order., Characteristics of true punters' pelvic girdles, such as laterally facing fin articulations, large surface area formuscle attachment, and tall lateral pelvic processes are similar to characteristics of early sprawled-gait tetrapods' pelvic girdles. This dissertation demonstrates that punting is common in batoids, illustrates the convergent evolution of true punter and early tetrapod pelvic anatomy, and gives possible explanations for the restriction of elasmobranchs to aquatic habitats.
Show less - Date Issued
- 2011
- PURL
- http://purl.flvc.org/FAU/3171678
- Subject Headings
- Sharks, Ecology, Fins, Anatomy, Adaptation (Biology), Aquatic animals, Physiology, Fishes, Locomotion
- Format
- Document (PDF)
- Title
- Magnetic orientation of loggerhead sea turtle hatchlings: migratory strategies in the Gulf of Mexico.
- Creator
- Merrill, Maria W., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Loggerhead sea turtles nest on either the Atlantic or Gulf coast of Florida. The hatchlings from these nests migrate offshore in opposite directions. The purpose of my study was to determine if Gulf coast hatchlings use magnetic maps, as Atlantic coast hatchlings do, both to locate areas favorable for survival in the Gulf of Mexico and to orient appropriately within surface currents that could transport them into the Atlantic Ocean. To find out, I presented Gulf coast hatchlings with magnetic...
Show moreLoggerhead sea turtles nest on either the Atlantic or Gulf coast of Florida. The hatchlings from these nests migrate offshore in opposite directions. The purpose of my study was to determine if Gulf coast hatchlings use magnetic maps, as Atlantic coast hatchlings do, both to locate areas favorable for survival in the Gulf of Mexico and to orient appropriately within surface currents that could transport them into the Atlantic Ocean. To find out, I presented Gulf coast hatchlings with magnetic fields corresponding to different locations inside the Gulf, and within currents leading into (Florida Straits) and within (Gulf Stream) the western portion of the Atlantic Ocean. I conclude that Gulf coast hatchlings (i) use a high resolution magnetic map for navigation within the Gulf of Mexico, (ii) initially remain within the eastern Gulf, but later may (iii) gain entry into currents that transport them into Atlantic waters.
Show less - Date Issued
- 2010
- PURL
- http://purl.flvc.org/FAU/2138106
- Subject Headings
- Animal orientation, Magnetic fields, Sea turtles, Migration, Loggerhead turtle, Migration
- Format
- Document (PDF)
- Title
- Highwire's characterization and signaling roles in Drosophila central synapse formation.
- Creator
- Rowland, Kimberly Diane., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
The assembly and maintenance of central synapses is a complex process, requiring myriad genes and their products. Highwire is a large gene containing a RING domain, characteristic of ubiquitin E3 ligases. Highwire has been shown to restrain axon growth and control synaptogenesis at a peripheral synapse. Here I examine the roles of Highwire at a central synapse in the adult Drosophila Giant Fiber System (GFS). Highwire is indeed necessary for proper axonal growth as well as synaptic...
Show moreThe assembly and maintenance of central synapses is a complex process, requiring myriad genes and their products. Highwire is a large gene containing a RING domain, characteristic of ubiquitin E3 ligases. Highwire has been shown to restrain axon growth and control synaptogenesis at a peripheral synapse. Here I examine the roles of Highwire at a central synapse in the adult Drosophila Giant Fiber System (GFS). Highwire is indeed necessary for proper axonal growth as well as synaptic transmission in the GFS. Differences arise between the central synapse and the neuromuscular junction (NMJ), where highwire was initially characterized : expresion from the postsynaptic cell can rescue highwire synaptic defects, which is not seen at the NMJ. In addition, a MAP kinase signaling pathway regulated by highwire at the NMJ has differing roles at a central synapse. Wallenda MAPK can rescue not only the highwire anatomical phenotype but also the defects seen in transmission. Another distinction is seen here : loss of function basket and Dfos enhance the highwire anatomical phenotype while expression of dominant negative basket and Dfos suppress the highwire phenotype. As a result we have compared the signaling pathway in flies and worms and found that the NMJ in the two organisms use a parallel pathway while the central synapse uses a distinct pathway.
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3352826
- Subject Headings
- Cellular control mechanisms, Cellular signal transduction, Cell differentiation, Gene expression, Genetic transcription, Transcription factors, Drosophila melanogaster, Cytogenetics
- Format
- Document (PDF)
- Title
- Higher order commutators in the method of orbits.
- Creator
- Kasprikova, Eva., Charles E. Schmidt College of Science, Department of Mathematical Sciences
- Abstract/Description
-
Benson spaces of higher order are introduced extending the idea of N. Krugljak and M. Milman, A distance between orbits that controls commutator estimates and invertibilty of operators, Advances in Mathematics 182 (2004), 78-123. The concept of Benson shift operators is introduced and a class of spaces equipped with these operators is considered. Commutator theorems of higher order on orbit spaces generated by a single element are proved for this class. It is shown that these results apply to...
Show moreBenson spaces of higher order are introduced extending the idea of N. Krugljak and M. Milman, A distance between orbits that controls commutator estimates and invertibilty of operators, Advances in Mathematics 182 (2004), 78-123. The concept of Benson shift operators is introduced and a class of spaces equipped with these operators is considered. Commutator theorems of higher order on orbit spaces generated by a single element are proved for this class. It is shown that these results apply to the complex method of interpolation and to the real method of interpolation for the case q=1. Two new characterizations are presented of the domain space of the "derivation" operator in the context of orbital methods. Comparisons to the work of others are made, especially the unifying paper of M. Cwikel, N. Kalton, M. Milman and R. Rochberg, A United Theory of Commutator Estimates for a Class of Interpolation Methods, Advances in Mathematics 169 2002, 241-312.
Show less - Date Issued
- 2009
- PURL
- http://purl.flvc.org/FAU/2684304
- Subject Headings
- Operator theory, Interpolation spaces, Finite groups, Sporadic groups (Mathematics)
- Format
- Document (PDF)
- Title
- Home on the range: spatial ecology of Atlantic spotted dolphins (Stenella frontalis) in the Bahamas.
- Creator
- Augliere, Bethany., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
I examined summer home range use of Altantic spotted dolphins in the Bahamas from 1991-2004. Home ranges for 86 dolphins using the 95% Fixed Kernel Density (FKD) estimator averages 62.o km2+=22.96 km2 and ranged from 24.8 km2 to 148.4 km2. Older animals and males had the largest ranges. Home range size did not vary with female reproductive status. core areas were defined by the 50% FKD contour and averaged 10.32 km2 +=5.47 km2. Habitat use was different between reproductive states for females...
Show moreI examined summer home range use of Altantic spotted dolphins in the Bahamas from 1991-2004. Home ranges for 86 dolphins using the 95% Fixed Kernel Density (FKD) estimator averages 62.o km2+=22.96 km2 and ranged from 24.8 km2 to 148.4 km2. Older animals and males had the largest ranges. Home range size did not vary with female reproductive status. core areas were defined by the 50% FKD contour and averaged 10.32 km2 +=5.47 km2. Habitat use was different between reproductive states for females. Non-reproductively active (NRA) females used deeped habitat. Behavioral use of the home range varied spatially. The core area was an important foraging area for males and reproductively active females. NRA females foraged more outside their core area compared to the core area.
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3358277
- Subject Headings
- Social behavior of animals, Dolphins, Geographical distribution, Dolphins, Habitat
- Format
- Document (PDF)
- Title
- Hatchling sex ratios and nest temperature-sex ratio response of three South Florida marine turtle species (Caretta caretta L., Chelona mydas L., and Dermochelys coriacea V.).
- Creator
- Rogers, Micah Marie., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
South Florida's loggerhead (Caretta caretta), green (Chelonia mydas) and leatherback (Dermochelys coriacea) sea turtles hatchling have environmentally determined sex. The in situ nest mean hatchling sex ratios (SR) were highly female-biased : loggerhead F=0.89) and green turtle F=0.81; leatherback's SR was nearly balanced (0.55F). Nest temperatures and SRs differed between leatherbacks and loggerhead and green turtles. The latter two did not differ. The loggerhead response parameters were...
Show moreSouth Florida's loggerhead (Caretta caretta), green (Chelonia mydas) and leatherback (Dermochelys coriacea) sea turtles hatchling have environmentally determined sex. The in situ nest mean hatchling sex ratios (SR) were highly female-biased : loggerhead F=0.89) and green turtle F=0.81; leatherback's SR was nearly balanced (0.55F). Nest temperatures and SRs differed between leatherbacks and loggerhead and green turtles. The latter two did not differ. The loggerhead response parameters were estimated within biological limitations by both 50-65% of incubation and mean middle 1/3 temperature. The maximum middle 1/3 temperature was the best-fit predictor for green turtles. No best-fit sex ratio-temperature response could be identified for leatherbacks. Clutches incubating under natural conditions can vary greatly in SR ; TRT differences may account for differences among species' sex ratios.
Show less - Date Issued
- 2013
- PURL
- http://purl.flvc.org/fcla/dt/3361062
- Subject Headings
- Sex ratio, Sex determination, Genetic, Sea turtles, Nests, Sea turtles, Embryology, Loggerhead turtle, Nests, Loggerhead turtle, Effect of temperature on
- Format
- Document (PDF)
- Title
- Hierarchical resource selection and movement of two wading bird species with divergent foraging strategies in the Everglades.
- Creator
- Beerens, James M., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Seasonal variation in food availability is one of the primary limitations to avian populations, particularly during the breeding season. However, the behavioral responses between species may differ based on foraging strategies. I examined the influence of food availability on landscape-level habitat selection, patch-level habitat selection, and movements of two wading bird species with divergent foraging strategies, the Great Egret and White Ibis. On a landscape scale, there appeared to be a...
Show moreSeasonal variation in food availability is one of the primary limitations to avian populations, particularly during the breeding season. However, the behavioral responses between species may differ based on foraging strategies. I examined the influence of food availability on landscape-level habitat selection, patch-level habitat selection, and movements of two wading bird species with divergent foraging strategies, the Great Egret and White Ibis. On a landscape scale, there appeared to be a relationship among resource availability, the temporal scale of the independent variable, and whether the response was similar or different between species. At the patch level, results demonstrated a relationship between resource availability and the spatial scale of the independent variables selected by birds. Species movements were consistent with the differing strategies. This study is the first to make the link between landscape hydrology patterns, prey availability, and responses in wading bird habitat selection at multiple spatial scales.
Show less - Date Issued
- 2008
- PURL
- http://purl.flvc.org/FAU/172667
- Subject Headings
- Water birds, Habitat, Habitat selection, Bird populations, Wetland ecology
- Format
- Document (PDF)
- Title
- Maternal and alloparental discipline in Atlantic spotted dolphins (Stenella frontalis) in the Bahamas.
- Creator
- Weinpress, Meghan., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Discipline was implemented by mothers and alloparent spotted dolphins (Stenella frontalis) mothers and alloparents on Little Bahama Bank, Bahamas. Disciplinarians were significantly more likely to be adults than juveniles. Although most disciplinarians were female, males were also observed to perform discipline. The recipients of discipline were male and female, and significantly more likely to be calves than juveniles. Pursuit, contact, and display behaviors were used in discipline, however...
Show moreDiscipline was implemented by mothers and alloparent spotted dolphins (Stenella frontalis) mothers and alloparents on Little Bahama Bank, Bahamas. Disciplinarians were significantly more likely to be adults than juveniles. Although most disciplinarians were female, males were also observed to perform discipline. The recipients of discipline were male and female, and significantly more likely to be calves than juveniles. Pursuit, contact, and display behaviors were used in discipline, however pursuit behaviors were most often observed. Variables such as age class, sex, and parity were not found to influence how discipline was implemented. The durations of all disciplinary pursuits were under thirty seconds, and successful pursuits had slightly shorter duration than unsuccessful pursuits. Disciplinarian success was not significantly influenced by age class, sex, parity, or behavior used.
Show less - Date Issued
- 2013
- PURL
- http://purl.flvc.org/fcla/dt/3361254
- Subject Headings
- Animal behavior, Behavior evolution, Atlantic mammals, Behavior, Parent-child relationships, Social behavior in animals, Discipline of children, Atlantic spotted dolphin
- Format
- Document (PDF)
- Title
- Mathematical modeling of plankton patchiness.
- Creator
- Ather, Simantha S., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
In natural systems, it has been observed that plankton exist in patches rather than in an even distribution across a body of water. However, the mechanisms behind this patchiness are not fully understood. Several previous modeling studies have examined the effects of abiotic and biotic factors on patch structure. Yet these models ignore a key point: zooplankton often undergo diel vertical migration. I have formulated a model that incorporates vertical movement into the Rosezweig-MacArthur (R...
Show moreIn natural systems, it has been observed that plankton exist in patches rather than in an even distribution across a body of water. However, the mechanisms behind this patchiness are not fully understood. Several previous modeling studies have examined the effects of abiotic and biotic factors on patch structure. Yet these models ignore a key point: zooplankton often undergo diel vertical migration. I have formulated a model that incorporates vertical movement into the Rosezweig-MacArthur (R-M) predator-prey model. The R-M model is stable only at a carrying capacity below a critical value. I found that adding vertical movement stabilizes the system even at a high carrying capacity. By analyzing temporal stability and spatial structure, my results show that vertical movement interacts with carrying capacity to determine patch structure.
Show less - Date Issued
- 2009
- PURL
- http://purl.flvc.org/FAU/368615
- Subject Headings
- Marine plankton, Vertical distribution, Marine ecology, Mathematical models, Macroecology, Mathematical models
- Format
- Document (PDF)
- Title
- Molecular mechanisms of neuroprotection in the anoxia tolerant freshwater turtle.
- Creator
- Kesaraju, Shailaja., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Cardiac ischemia, stroke and some neurodegenerative disorders are all characterized by cell damage and death due to low oxygen levels. Comparative studies show that anoxia tolerant model systems present a unique opportunity to study "survival" instead of death in the complete absence of oxygen. The freshwater turtle (Trachemys scripta elegans) is unique in its ability to survive total oxygen deprivation for hours to days, as well as reoxygenation insult after anoxia. The broad objective of...
Show moreCardiac ischemia, stroke and some neurodegenerative disorders are all characterized by cell damage and death due to low oxygen levels. Comparative studies show that anoxia tolerant model systems present a unique opportunity to study "survival" instead of death in the complete absence of oxygen. The freshwater turtle (Trachemys scripta elegans) is unique in its ability to survive total oxygen deprivation for hours to days, as well as reoxygenation insult after anoxia. The broad objective of this study is to understand the modulation of key molecular mechanisms involving stress proteins and VEGF that offer neuroprotection and enhance cell survival in the freshwater turtle through anoxia and reoxygenation. In vivo analyses have shown that anoxia induced stress proteins (Hsp72, Hsp60, Grp94, Hsp60, Hsp27, HO-1); modest changes in the Bcl2/Bax ratio and no change in cleaved caspase-3 expression suggesting resistance to neuronal damage. These results were corroborated with immunohistochemical evidence indicating no damage in turtle brain when subjected to the stress of anoxia and A/R. To understand the functional role of Hsp72, siRNA against Hsp72 was utilized to knockdown Hsp72 in vitro (neuronally enriched primary cell cultures established from the turtle). Knockdown cultures were characterized by increased cell death associated with elevated ROS levels. Silencing of Hsp72 knocks down the expression of Bcl2 and increases the expression of Bax, thereby decreasing the Bcl2/Bax ratio. However, there was no increase in cytosolic Cytochrome c or the expression levels of cleaved Caspase-3. Significant increase in AIF was observed in the knockdown cultures that increase through anoxia and reoxygenation, suggesting a caspase independent pathway of cell death., Expression of the master regulator of hypoxia, HIF1 alpha and its target gene, VEGF, were analyzed at the mRNA and protein levels. The results showed no significant increase in HIF-1 alpha levels but anoxia VE GF The levels of stress proteins and VEGF returned to control levels during reoxygenation suggesting robust ROS protection mechanisms through reoxygenation. The present study thereby emphasizes Trachemys scripta as an advantageous model to examine anoxia and reoxygenation survival without major damage to the brain due to it's modulation of molecular mechanisms.
Show less - Date Issued
- 2008
- PURL
- http://purl.flvc.org/FAU/165943
- Subject Headings
- Turtles, Physiology, Anoxemia, Proteins, Chemical modification, Oxygen, Physiological effect, Molecular neurobiology
- Format
- Document (PDF)