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Title: Algenwachstum in ozeanischen Tiefen [Plant life of the deep ocean realm].
Creator: Littler, Mark M., Littler, Diane S., Harbor Branch Oceanographic Institute
Date Issued: 1994
PURL: http://purl.flvc.org/FCLA/DT/3342315
Subject Headings: Deep-sea biology, Algae--Growth, Aquatic plants
Format: Document (PDF)

Title: Reconstruction of Family-Level Phylogenetic Relationships within Demospongiae (Porifera) Using Nuclear Encoded Housekeeping Genes.
Creator: Hill, Malcolm S., Hill, April L., Lopez, Jose V., Peterson, Kevin J., Pomponi, Shirley A., Diaz, Maria Cristina, Thacker, Robert W., Adamska, Maja, Boury-Esnault, Nicole, Cárdenas, Paco, Chaves-Fonnegra, Andia, Danka, Elizabeth, De Laine, Bre-Onna, Formica, Dawn, Hajdu, Eduardo, Lobo-Hajdu, Gisele, Klontz, Sarah, Morrow, Christine C., Patel, Jignasa, Picton, Bernard, Pisani, Davide, Pohlmann, Deborah, Redmond, Niamh E., Reed, John K., Richey, Stacy, Riesgo, Ana, Rubin, Ewelina, Russell, Zach, Rützler, Klaus, Sperling, Erik A., di Stefano, Michael, Tarver, James E., Collins, Allen G., Lin, Senjie
Abstract/Description: Background: Demosponges are challenging for phylogenetic systematics because of their plastic and relatively simple morphologies and many deep divergences between major clades. To improve understanding of the phylogenetic relationships within Demospongiae, we sequenced and analyzed seven nuclear housekeeping genes involved in a variety of cellular functions from a diverse group of sponges. Methodology/Principal Findings: We generated data from each of the four sponge classes (i.e., Calcarea,...
Show moreBackground: Demosponges are challenging for phylogenetic systematics because of their plastic and relatively simple morphologies and many deep divergences between major clades. To improve understanding of the phylogenetic relationships within Demospongiae, we sequenced and analyzed seven nuclear housekeeping genes involved in a variety of cellular functions from a diverse group of sponges. Methodology/Principal Findings: We generated data from each of the four sponge classes (i.e., Calcarea, Demospongiae, Hexactinellida, and Homoscleromorpha), but focused on family-level relationships within demosponges. With data for 21 newly sampled families, our Maximum Likelihood and Bayesian-based approaches recovered previously phylogenetically defined taxa: Keratosap, Myxospongiaep, Spongillidap, Haploscleromorphap (the marine haplosclerids) and Democlaviap. We found conflicting results concerning the relationships of Keratosap and Myxospongiaep to the remaining demosponges, but our results strongly supported a clade of Haploscleromorphap+Spongillidap+Democlaviap. In contrast to hypotheses based on mitochondrial genome and ribosomal data, nuclear housekeeping gene data suggested that freshwater sponges (Spongillidap) are sister to Haploscleromorphap rather than part of Democlaviap. Within Keratosap, we found equivocal results as to the monophyly of Dictyoceratida. Within Myxospongiaep, Chondrosida and Verongida were monophyletic. A wellsupported clade within Democlaviap, Tetractinellidap, composed of all sampled members of Astrophorina and Spirophorina (including the only lithistid in our analysis), was consistently revealed as the sister group to all other members of Democlaviap. Within Tetractinellidap, we did not recover monophyletic Astrophorina or Spirophorina. Our results also reaffirmed the monophyly of order Poecilosclerida (excluding Desmacellidae and Raspailiidae), and polyphyly of Hadromerida and Halichondrida. Conclusions/Significance: These results, using an independent nuclear gene set, confirmed many hypotheses based on ribosomal and/or mitochondrial genes, and they also identified clades with low statistical support or clades that conflicted with traditional morphological classification. Our results will serve as a basis for future exploration of these outstanding questions using more taxon- and gene-rich datasets.
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Date Issued: 2013-01-23
PURL: http://purl.flvc.org/fau/fd/FAUIR000044
Format: Citation

Title: Satellite-Observed Black Water Events off Southwest Florida: Implications for Coral Reef Health in the Florida Keys National Marine Sanctuary.
Creator: Zhao, Jun, Hu, Chuanmin, Lapointe, Brian E., Melo, Nelson, Johns, Elizabeth, Smith, Ryan
Abstract/Description: A “black water” event, as observed from satellites, occurred off southwest Florida in 2012. Satellite observations suggested that the event started in early January and ended in mid-April 2012. The black water patch formed off central west Florida and advected southward towards Florida Bay and the Florida Keys with the shelf circulation, which was confirmed by satellite-tracked surface drifter trajectories. Compared with a previous black water event in 2002, the 2012 event was weaker in terms...
Show moreA “black water” event, as observed from satellites, occurred off southwest Florida in 2012. Satellite observations suggested that the event started in early January and ended in mid-April 2012. The black water patch formed off central west Florida and advected southward towards Florida Bay and the Florida Keys with the shelf circulation, which was confirmed by satellite-tracked surface drifter trajectories. Compared with a previous black water event in 2002, the 2012 event was weaker in terms of spatial and temporal coverage. An in situ survey indicated that the 2012 black water patch contained toxic K. brevis and had relatively low CDOM (colored dissolved organic matter) and turbidity but high chlorophyll-a concentrations, while salinity was somewhat high compared with historical values. Further analysis revealed that the 2012 black water was formed by the K. brevis bloom initiated off central west Florida in late September 2011, while river runoff, Trichodesmium and possibly submarine groundwater discharge also played important roles in its formation. Black water patches can affect benthic coral reef communities by decreasing light availability at the bottom, and enhanced nutrient concentrations from black water patches support massive macroalgae growth that can overgrow coral reefs. It is thus important to continue the integrated observations where satellites provide synoptic and repeated observations of such adverse water quality events.
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Date Issued: 2013-01-18
PURL: http://purl.flvc.org/fau/fd/FAUIR000032
Format: Citation

Title: Separation and HPLC Analysis of Diastereomers and Rotational Isomers of.
Creator: Chamely-Wiik, Donna M., Carraher, Charles E., Kamel, George, Haky, Jerome E.
Date Issued: 2006-06-10
PURL: http://purl.flvc.org/fau/flvc_fau_islandoraimporter_10.1080_10826070600757680_1521555358
Format: Citation

Title: The Marine Natural Product Manzamine A Targets Vacuolar ATPases and Inhibits Autophagy in Pancreatic Cancer Cells.
Creator: Kallifatidis, Georgios, Hoepfner, Dominic, Jaeg, Tiphaine, Guzman, Esther A., Wright, Amy E.
Abstract/Description: Manzamine A, a member of the manzamine alkaloids, was originally isolated from marine sponges of the genus Haliclona. It was recently shown to have activity against pancreatic cancer cells, but the precise mechanism of action remained unclear. To further our understanding of the mechanism of action of manzamine A, chemogenomic profiling in the yeast S. cerevisiae was performed, suggesting that manzamine A is an uncoupler of vacuolar ATPases. Fluorescence microscopy confirmed this effect on...
Show moreManzamine A, a member of the manzamine alkaloids, was originally isolated from marine sponges of the genus Haliclona. It was recently shown to have activity against pancreatic cancer cells, but the precise mechanism of action remained unclear. To further our understanding of the mechanism of action of manzamine A, chemogenomic profiling in the yeast S. cerevisiae was performed, suggesting that manzamine A is an uncoupler of vacuolar ATPases. Fluorescence microscopy confirmed this effect on yeast vacuoles, where manzamine A produced a phenotype very similar to that of the established v-ATPase inhibitor bafilomycin A1. In pancreatic cancer cells, 10 μM manzamine A affected vacuolar ATPase activity and significantly increased the level of autophagosome marker LC3-II and p62/SQSTM1 as observed by western blot analysis. Treatment with manzamine A in combination with bafilomycin A1 (inhibitor of autophagosome-lysosome fusion) did not change the levels of LC3-II when compared to cells treated with bafilomycin A1 alone, suggesting that manzamine A is a potential inhibitor of autophagy by preventing autophagosome turnover. As autophagy is essential for pancreatic tumor growth, blocking this pathway with manzamine A suggests a promising strategy for the treatment of pancreatic cancer.
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Date Issued: 2013-09-17
PURL: http://purl.flvc.org/fau/fd/FAUIR000075
Format: Citation

Title: Correction: Kallifatidis, G. et al. The Marine Natural Product Manzamine A Targets Vacuolar ATPases and Inhibits Autophagy in Pancreatic Cancer Cells. Mar. Drugs 2013, 11, 3500–3516.
Creator: Kallifatidis, Georgios, Hoepfner, Dominic, Jaeg, Tiphaine, Guzman, Esther A., Wright, Amy E.
Abstract/Description: We found two errors in our previous published paper [1]. Figure 4A has a mistake in the units in the labels, where it shows mM instead of micromolar (μM). A correctly labeled Figure 4A ensues. In Figures 2 and 4, the size bar scale is micrometers (μm). We apologize for the inconvenience caused to our readers.
Date Issued: 2014-04-21
PURL: http://purl.flvc.org/fau/fd/FAUIR000076
Format: Citation

Title: Spongiatriol Inhibits Nuclear Factor Kappa B Activation and Induces Apoptosis in Pancreatic Cancer Cells.
Creator: Guzman, Esther A., Maher, Michael, Temkin, Alexis, Pitts, Tara P., Wright, Amy E.
Date Issued: 2013-04-02
PURL: http://purl.flvc.org/fau/fd/FAUIR000178
Format: Citation

Title: Concentracion deiones requirida para el cultivo de Litopenaeus vannamei en agua dulce.
Creator: Laramore, Susan E., Scarpa, John, McGraw, W. M.
Date Issued: 2003
PURL: http://purl.flvc.org/fau/fd/FA00007359
Format: Document (PDF)

Title: Hair Mercury Concentrations and Fish Consumption Patterns in Florida Residents.
Creator: Schaefer, Adam M., Jensen, Emily, Bossart, Gregory D., Reif, John S.
Date Issued: 2014-06-26
PURL: http://purl.flvc.org/fau/fd/FAUIR000186
Format: Citation

Title: Numerical simulations of a horizontal axis water turbine designed for underwater mooring platforms.
Creator: Tian, Wenlong, Song, Baowei, VanZwieten, James H., Pyakurel, Parakram, Li, Yanjun
Date Issued: 2016-01
PURL: http://purl.flvc.org/fau/fd/FAUIR000223
Format: Citation

Title: From Bhopal to Cold Fusion: A Case-Study Approach to Writing Assignments in Honors General Chemistry.
Creator: Chamely-Wiik, Donna M., Haky, Jerome E., Galin, Jeffrey R.
Date Issued: 2012-03-13
PURL: http://purl.flvc.org/fau/flvc_fau_islandoraimporter_10.1021_ed101129v_1521555359
Format: Citation

Title: Computational Fluid Dynamics Prediction of a Modified Savonius Wind Turbine with Novel Blade Shapes.
Creator: Tian, Wenlong, Song, Baowei, VanZwieten, James H., Pyakurel, Parakram
Date Issued: 2015-07-30
PURL: http://purl.flvc.org/fau/fd/FAUIR000195
Format: Citation

Title: Plant expression of cocaine hydrolase-Fc fusion protein for treatment of cocaine abuse.
Creator: Wang, Guojun, Zhang, Ting, Huang, Haifeng, Hou, Shurong, Chen, Xiabin, Zheng, Fang, Zhan, Chang-Guo
Date Issued: 2016-12-19
PURL: http://purl.flvc.org/fau/fd/FAUIR000197
Format: Citation

Title: Influence of Biological Factors on Connectivity Patterns for Concholepas concholepas (loco) in Chile.
Creator: Garavelli, Lysel, Colas, François, Verley, Philippe, Kaplan, David Michael, Yannicelli, Beatriz, Lett, Christophe, Chiang, Tzen-Yuh
Abstract/Description: In marine benthic ecosystems, larval connectivity is a major process influencing the maintenance and distribution of invertebrate populations. Larval connectivity is a complex process to study as it is determined by several interacting factors. Here we use an individual-based, biophysical model, to disentangle the effects of such factors, namely larval vertical migration, larval growth, larval mortality, adults fecundity, and habitat availability, for the marine gastropod Concholepas...
Show moreIn marine benthic ecosystems, larval connectivity is a major process influencing the maintenance and distribution of invertebrate populations. Larval connectivity is a complex process to study as it is determined by several interacting factors. Here we use an individual-based, biophysical model, to disentangle the effects of such factors, namely larval vertical migration, larval growth, larval mortality, adults fecundity, and habitat availability, for the marine gastropod Concholepas concholepas (loco) in Chile. Lower transport success and higher dispersal distances are observed including larval vertical migration in the model. We find an overall decrease in larval transport success to settlement areas from northern to southern Chile. This spatial gradient results from the combination of current direction and intensity, seawater temperature, and available habitat. From our simulated connectivity patterns we then identify subpopulations of loco along the Chilean coast, which could serve as a basis for spatial management of this resource in the future.
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Date Issued: 2016-01-11
PURL: http://purl.flvc.org/fau/fd/FAUIR000020
Format: Citation

Title: Ploidy Distribution of the Harmful Bloom Forming Macroalgae Ulva spp. in Narragansett Bay, Rhode Island, USA, Using Flow Cytometry Methods.
Creator: Potter, Elaine E., Thornber, Carol S., Swanson, John-David, McFarland, Malcolm, Gobler, Christopher J.
Abstract/Description: Macroalgal blooms occur worldwide and have the potential to cause severe ecological and economic damage. Narragansett Bay, RI is a eutrophic system that experiences summer macroalgal blooms composed mostly of Ulva compressa and Ulva rigida, which have biphasic life cycles with separate haploid and diploid phases. In this study, we used flow cytometry to assess ploidy levels of U. compressa and U. rigida populations from five sites in Narragansett Bay, RI, USA, to assess the relative...
Show moreMacroalgal blooms occur worldwide and have the potential to cause severe ecological and economic damage. Narragansett Bay, RI is a eutrophic system that experiences summer macroalgal blooms composed mostly of Ulva compressa and Ulva rigida, which have biphasic life cycles with separate haploid and diploid phases. In this study, we used flow cytometry to assess ploidy levels of U. compressa and U. rigida populations from five sites in Narragansett Bay, RI, USA, to assess the relative contribution of both phases to bloom formation. Both haploid gametophytes and diploid sporophytes were present for both species. Sites ranged from a relative overabundance of gametophytes to a relative overabundance of sporophytes, compared to the null model prediction of 2 gametophytes: 1 sporophyte. We found significant differences in cell area between ploidy levels for each species, with sporophyte cells significantly larger than gametophyte cells in U. compressa and U. rigida.We found no differences in relative growth rate between ploidy levels for each species. Our results indicate the presence of both phases of each of the two dominant bloom forming species throughout the bloom season, and represent one of the first studies of in situ Ulva life cycle dynamics.
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Date Issued: 2016-02-26
PURL: http://purl.flvc.org/fau/fd/FAUIR000037
Format: Citation

Title: Health evaluation of African penguins (Spheniscus demersus) in southern Africa.
Creator: Parsons, Nola J., Gous, Tertius A., Schaefer, Adam M., Vanstreels, Ralph E.T., Harbor Branch Oceanographic Institute
Abstract/Description: The African penguin (Spheniscus demersus) is an endangered seabird that breeds along the coast of Namibia and South Africa, and disease surveillance was identified as a priority for its conservation. Aiming for the establishment of baseline data on the presence of potential pathogens in this species, a comprehensive health assessment (blood smear examination, haematology, biochemistry and serology) was conducted on samples obtained from 578 African penguins at 11 breeding colonies and a...
Show moreThe African penguin (Spheniscus demersus) is an endangered seabird that breeds along the coast of Namibia and South Africa, and disease surveillance was identified as a priority for its conservation. Aiming for the establishment of baseline data on the presence of potential pathogens in this species, a comprehensive health assessment (blood smear examination, haematology, biochemistry and serology) was conducted on samples obtained from 578 African penguins at 11 breeding colonies and a rehabilitation centre. There were 68 penguins that were seropositive for at least one of seven pathogens tested: avian encephalomyelitis virus, avian infectious bronchitis virus, avian reovirus, infectious bursal disease virus, Newcastle disease virus, Mycoplasma gallisepticum and Mycoplasma synoviae. All samples were seronegative for avian influenza virus subtypes H5 and H7 and infectious laryngotracheitis virus. The apparent prevalence of Babesia sp. and Borrelia sp. in blood smears was consistent with previous studies. Babesia-infected individuals had a regenerative response of the erythrocytic lineage, an active inflammatory response and hepatic function impairment. These findings indicate that African penguins may be exposed to conservation-significant pathogens in the wild and encourage further studies aiming for the direct detection and/or isolation of these microorganisms.
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Date Issued: 2016-03-09
PURL: http://purl.flvc.org/fau/fd/FAUIR000049
Format: Citation

Title: Eastern Caribbean Circulation and Island Mass Effect on St. Croix, US Virgin Islands: A Mechanism for Relatively Consistent Recruitment Patterns.
Creator: Chérubin, Laurent Marcel, Garavelli, Lysel, Han, Guoqi, Harbor Branch Oceanographic Institute
Abstract/Description: The northeastern Caribbean Sea is under the seasonal influence of the Trade Winds but also of the Orinoco/Amazon freshwater plume. The latter is responsible for intensification of the Caribbean Current in general and of its eddy activity in the northern part of the Caribbean Sea. More importantly, we show in this study that the front of the freshwater plume drives a northward flow that impinges directly on the island of St. Croix in the United States Virgin Islands. The angle of incidence of...
Show moreThe northeastern Caribbean Sea is under the seasonal influence of the Trade Winds but also of the Orinoco/Amazon freshwater plume. The latter is responsible for intensification of the Caribbean Current in general and of its eddy activity in the northern part of the Caribbean Sea. More importantly, we show in this study that the front of the freshwater plume drives a northward flow that impinges directly on the island of St. Croix in the United States Virgin Islands. The angle of incidence of the incoming flow controls the nature of the wake on both sides and ends of the island, which changes from cyclonic to anticylonic wake flow, with either attached or shed eddies. Using an off-line bio-physical model, we simulated the dispersal and recruitment of an abundant Caribbean coral reef fish, the bluehead wrasse (Thalassoma bifasciatum) in the context of the wake flow variability around St. Croix. Our results revealed the role played by the consistent seasonal forcing of the wake flow on the recruitment patterns around the island at the interannual scale. The interannual variability of the timing of arrival and northward penetration of the plume instead controls the nature of the wake, hence the regional spatial recruitment patterns.
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Date Issued: 2016-03-04
PURL: http://purl.flvc.org/fau/fd/FAUIR000011
Format: Citation

Title: Establishment of baseline haematology and biochemistry parameters in wild adult African penguins (Spheniscus demersus).
Creator: Parsons, Nola J., Schaefer, Adam M., Van der Spuy, Stephen D., Gous, Tertius A.
Date Issued: 2015-03-25
PURL: http://purl.flvc.org/fau/fd/FAUIR000187
Format: Citation

Title: Natural Products from the Lithistida: A Review of the Literature since 2000.
Creator: Winder, Priscilla L., Pomponi, Shirley A., Wright, Amy E.
Abstract/Description: Lithistid sponges are known to produce a diverse array of compounds ranging from polyketides, cyclic and linear peptides, alkaloids, pigments, lipids, and sterols. A majority of these structurally complex compounds have very potent and interesting biological activities. It has been a decade since a thorough review has been published that summarizes the literature on the natural products reported from this amazing sponge order. This review provides an update on the current taxonomic...
Show moreLithistid sponges are known to produce a diverse array of compounds ranging from polyketides, cyclic and linear peptides, alkaloids, pigments, lipids, and sterols. A majority of these structurally complex compounds have very potent and interesting biological activities. It has been a decade since a thorough review has been published that summarizes the literature on the natural products reported from this amazing sponge order. This review provides an update on the current taxonomic classification of the Lithistida, describes structures and biological activities of 131 new natural products, and discusses highlights from the total syntheses of 16 compounds from marine sponges of the Order Lithistida providing a compilation of the literature since the last review published in 2002.
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Date Issued: 2011-12-15
PURL: http://purl.flvc.org/fau/fd/FAUIR000059
Format: Citation

Title: Two distinct microbial communities revealed in the sponge Cinachyrella.
Creator: Cuvelier, Marie L., Blake, Emily, Mulheron, Rebecca, McCarthy, Peter J., Blackwelder, Patricia, Thurber, Rebecca L. Vega, Lopez, Jose V.
Date Issued: 2014-11-04
PURL: http://purl.flvc.org/fau/fd/FAUIR000172
Format: Citation

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