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DELINEATING THE INTRACELLULAR AND EXTRACELLULAR ROLE OF MATRIX METALLOPROTEINASE 14 IN PRIMARY AND METASTATIC PANCREATIC CANCERS BxPC-3 AND HPAF-II

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Date Issued:
2023
Abstract/Description:
Using flow cytometry and two forms of hypoxic induction, chemical (CoCl2) and gaseous (Tri-gas chamber), this study highlighted the advantages of quantitative analyses of MMP-14 expression (intracellularly/extracellularly) for one primary (BxPC-3) and one metastatic (HPAF-II) pancreatic cancer line. No significant changes in MMP-14 expression were observed for pancreatic cancer lines using CoCl2. Increased expression levels of MMP-14 were observed using tri-gas incubation maintaining oxygen levels at 2% intracellularly for HPAF-II, but not BxPC-3. A combination of 2D/3D cell culturing techniques were also used to examine changes in cellular behavior/morphology after hypoxic exposure and a 24-hour reoxygenation cycle. BxPC-3 cells showed a greater propensity toward oxidative damage caused by reoxygenation using 2D culturing techniques. Using 3D biomimetic culturing techniques, reoxygenated BxPC-3 cells did not undergo significant apoptosis or necrosis. These results suggest that changes in cellular metabolism and behavior depend on both phenotype and culture scaffolding.
Title: DELINEATING THE INTRACELLULAR AND EXTRACELLULAR ROLE OF MATRIX METALLOPROTEINASE 14 IN PRIMARY AND METASTATIC PANCREATIC CANCERS BxPC-3 AND HPAF-II.
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Name(s): Gopi, Nihasika, author
Fields, Gregg B. , Thesis advisor
Harriet L. Wilkes Honors College
Florida Atlantic University, Degree grantor
Type of Resource: text
Genre: Thesis
Date Created: 2023
Date Issued: 2023
Publisher: Florida Atlantic University
Place of Publication: Jupiter, FL
Physical Form: application/pdf
Extent: 29 p.
Language(s): English
Abstract/Description: Using flow cytometry and two forms of hypoxic induction, chemical (CoCl2) and gaseous (Tri-gas chamber), this study highlighted the advantages of quantitative analyses of MMP-14 expression (intracellularly/extracellularly) for one primary (BxPC-3) and one metastatic (HPAF-II) pancreatic cancer line. No significant changes in MMP-14 expression were observed for pancreatic cancer lines using CoCl2. Increased expression levels of MMP-14 were observed using tri-gas incubation maintaining oxygen levels at 2% intracellularly for HPAF-II, but not BxPC-3. A combination of 2D/3D cell culturing techniques were also used to examine changes in cellular behavior/morphology after hypoxic exposure and a 24-hour reoxygenation cycle. BxPC-3 cells showed a greater propensity toward oxidative damage caused by reoxygenation using 2D culturing techniques. Using 3D biomimetic culturing techniques, reoxygenated BxPC-3 cells did not undergo significant apoptosis or necrosis. These results suggest that changes in cellular metabolism and behavior depend on both phenotype and culture scaffolding.
Identifier: FAUHT00284 (IID)
Degree granted: Thesis (B.S.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2023
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FAUHT00284
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Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.

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