You are here

FAU Collections » FAU Research Repository » FAU Theses and Dissertations

Resistant or persistent reovirus infections: Of normal human embryonic lung fibroblast WI-38 cells

Download pdf | Full Screen View

Date Issued:
2003
Summary:
Reovirus is a common virus that usually affects children; this infection causes symptoms such as respiratory and or gastrointestinal aliments. Morbidity most often occurs in impoverished countries where supportive hospitalization is not available. In the U.S. and other established countries morbidity is not an issue. When WI-38 cells are infected with reovirus the infection is either resisted by the cells or a persistent latent infection occurs. In this study, gene expression was analyzed by comparing Reovirus-infected WI-38 cells with mock infected cells. P.R.O.M(TM) analysis was performed on RNA sent to Genka Biotechnology Inc. Bioinformatics was used to analyze the data. Reovirus infection of the WI-38 cells resulted in increased mRNA levels for a number of transcription regulatory genes, and possibly more significant, decreased mRNA levels for some very important regulatory genes. These changes may be responsible for establishing the antiviral replication environment observed in these normal cells.
Title: Resistant or persistent reovirus infections: Of normal human embryonic lung fibroblast WI-38 cells.
124 views
35 downloads
Name(s): Eukitis, Martine Marie.
Florida Atlantic University, Degree grantor
Roner, Michael R., Thesis advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 2003
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 78 p.
Language(s): English
Summary: Reovirus is a common virus that usually affects children; this infection causes symptoms such as respiratory and or gastrointestinal aliments. Morbidity most often occurs in impoverished countries where supportive hospitalization is not available. In the U.S. and other established countries morbidity is not an issue. When WI-38 cells are infected with reovirus the infection is either resisted by the cells or a persistent latent infection occurs. In this study, gene expression was analyzed by comparing Reovirus-infected WI-38 cells with mock infected cells. P.R.O.M(TM) analysis was performed on RNA sent to Genka Biotechnology Inc. Bioinformatics was used to analyze the data. Reovirus infection of the WI-38 cells resulted in increased mRNA levels for a number of transcription regulatory genes, and possibly more significant, decreased mRNA levels for some very important regulatory genes. These changes may be responsible for establishing the antiviral replication environment observed in these normal cells.
Identifier: 9780496179282 (isbn), 12991 (digitool), FADT12991 (IID), fau:9858 (fedora)
Collection: FAU Electronic Theses and Dissertations Collection
Note(s): Thesis (M.S.)--Florida Atlantic University, 2003.
Charles E. Schmidt College of Science
Subject(s): Reoviruses
Genetic regulation
Fibroblasts
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/12991
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.