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MICROFLUIDIC ELECTRICAL IMPEDANCE TECHNOLOGY FOR POINT-OFCARE ASSESSMENT OF SICKLE CELL DISEASE
| Title: | MICROFLUIDIC ELECTRICAL IMPEDANCE TECHNOLOGY FOR POINT-OFCARE ASSESSMENT OF SICKLE CELL DISEASE. |
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| Name(s): |
Dieujuste, Darryl , author Du, Sarah, Thesis advisor Florida Atlantic University, Degree grantor Department of Ocean and Mechanical Engineering College of Engineering and Computer Science |
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| Type of Resource: | text | |
| Genre: | Electronic Thesis Or Dissertation | |
| Date Created: | 2023 | |
| Date Issued: | 2023 | |
| Publisher: | Florida Atlantic University | |
| Place of Publication: | Boca Raton, Fla. | |
| Physical Form: | application/pdf | |
| Extent: | 85 p. | |
| Language(s): | English | |
| Abstract/Description: | Sickle Cell Disease (SCD) is a genetic disease that affects approximately 100,000 people in the USA and millions worldwide. The disease is defined by a mutation in hemoglobin, the red blood cell’s oxygen carrying component. Under hypoxic (low oxygen) conditions, the mutated hemoglobin (known as HbS) polymerizes into rigid fibers that stretch the cell into a sickle shape. These rigid cells can occlude blood vessels and cause an individual immense pain. Currently, no point-of-care devices exist in the market for assisting those with SCD. Using microfluidics with custom designed portable impedance measuring hardware we can achieve label-free in vitro analyses of SCD rheology. This dissertation presents two impedance-based devices for finger-prick volume blood testing, including a microflow cytometer for SCD diagnostics and a vaso-occlusion tester for monitoring blood flow activities. First, the microflow cytometer is validated by measuring the electrical impedance of individual cells flowing through a narrow microfluidic channel. Cellular impedance is interpreted by changes in subcellular components due to oxygen association-dissociation of hemoglobin, using an equivalent circuit model and Multiphysics simulation. Impedance values of sickle cells exhibit remarkable deviations from normal blood cells. Such deviation is quantified by a conformity score, which allows for measurement of SCD heterogeneity, and potentially disease severity. Findings from this study demonstrate the potential for SCD screening via electrical impedance. Second, a vaso-occlusion tester is validated by measuring the impedance response of blood flow within a microfluidic mimic of capillary bed. | |
| Identifier: | FA00014243 (IID) | |
| Degree granted: | Dissertation (PhD)--Florida Atlantic University, 2023. | |
| Collection: | FAU Electronic Theses and Dissertations Collection | |
| Note(s): | Includes bibliography. | |
| Subject(s): |
Sickle cell anemia Microfluidics Point-of-care testing Electric Impedance |
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| Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00014243 | |
| Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
| Host Institution: | FAU |
