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stability study of octadecylalumina columns for use in HPLC

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Date Issued:
2000
Summary:
Chromatographic stability studies of an alumina-based reversed phase, ODA, stationary phase were performed. These studies involved monitoring the chromatographic performance of columns packed with the ODA phase after separate, continuous treatment with aqueous solutions of trifluoroacetic acid and sodium hydroxide over a period of several weeks. Chromatographic performance of the ODA columns undergoing treatment with these mobile phases was evaluated by comparing changes in chromatographic capacity factors, resolutions, plate numbers, and peak asymmetries of a test mixture consisting of uracil, phenol, aniline, methyl benzoate, m-toluidine, N,N-dimethylaniline, and toluene. The results indicate that the alumina-based ODA phase is stable under acidic conditions (pH 2--3) and exceptionally stable to chemical degradation under alkaline conditions (pH ∼ 11).
Title: A stability study of octadecylalumina columns for use in HPLC.
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Name(s): Goldberg, James Vincent
Florida Atlantic University, Degree Grantor
Haky, Jerome E., Thesis Advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 2000
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 98 p.
Language(s): English
Summary: Chromatographic stability studies of an alumina-based reversed phase, ODA, stationary phase were performed. These studies involved monitoring the chromatographic performance of columns packed with the ODA phase after separate, continuous treatment with aqueous solutions of trifluoroacetic acid and sodium hydroxide over a period of several weeks. Chromatographic performance of the ODA columns undergoing treatment with these mobile phases was evaluated by comparing changes in chromatographic capacity factors, resolutions, plate numbers, and peak asymmetries of a test mixture consisting of uracil, phenol, aniline, methyl benzoate, m-toluidine, N,N-dimethylaniline, and toluene. The results indicate that the alumina-based ODA phase is stable under acidic conditions (pH 2--3) and exceptionally stable to chemical degradation under alkaline conditions (pH ∼ 11).
Identifier: 9780599813724 (isbn), 12681 (digitool), FADT12681 (IID), fau:9563 (fedora)
Note(s): Thesis (M.S.)--Florida Atlantic University, 2000.
Subject(s): Chromatographic analysis
High performance liquid chromatography
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/12681
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
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Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.