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Origin and mechanism of terpene biosynthesis in Pseudopterogorgia spp

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Date Issued:
2006
Summary:
The goal of this work was to investigate the biosynthetic origins of diterpene natural products (pseudopterosins, kallolides, bipinnatins, and cembrenes) from corals of the genus Pseudopterogorgia as well as the biosynthetic pathways by which they are produced. These studies have shown that the pseudopterosins from Pseudopterogorgia elisabethae are biosynthesized within the algal symbiont (or possibly a bacterium or fungus associated with the symbiont), are not inducible by manipulation of light levels, and do not change as a result of transplantation to new locations. Studies on Pseudopterogorgia bipinnata revealed that only one chemotype is capable of biosynthesizing the kallolide family of diterpenes. The biosynthetic pathway which gives rise to the kallolides has been shown to involve members of another family of diterpenes, the bipinnatins, which coexist within the coral holobiont. Two diterpene cyclase products have been discovered within P. bipinnata chemotype A, cembrene and neocembrene, and it has been shown that neocembrene gives rise to the kallolides. Finally, the enzymatic conversion of bipinnatin J to kallolide A has shown for the first time that these compounds are in fact biogenetically related.
Title: Origin and mechanism of terpene biosynthesis in Pseudopterogorgia spp.
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Name(s): Boehnlein, Jennifer M.
Florida Atlantic University, Degree Grantor
Kerr, Russell G., Thesis Advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 2006
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 142 p.
Language(s): English
Summary: The goal of this work was to investigate the biosynthetic origins of diterpene natural products (pseudopterosins, kallolides, bipinnatins, and cembrenes) from corals of the genus Pseudopterogorgia as well as the biosynthetic pathways by which they are produced. These studies have shown that the pseudopterosins from Pseudopterogorgia elisabethae are biosynthesized within the algal symbiont (or possibly a bacterium or fungus associated with the symbiont), are not inducible by manipulation of light levels, and do not change as a result of transplantation to new locations. Studies on Pseudopterogorgia bipinnata revealed that only one chemotype is capable of biosynthesizing the kallolide family of diterpenes. The biosynthetic pathway which gives rise to the kallolides has been shown to involve members of another family of diterpenes, the bipinnatins, which coexist within the coral holobiont. Two diterpene cyclase products have been discovered within P. bipinnata chemotype A, cembrene and neocembrene, and it has been shown that neocembrene gives rise to the kallolides. Finally, the enzymatic conversion of bipinnatin J to kallolide A has shown for the first time that these compounds are in fact biogenetically related.
Identifier: 9780542579448 (isbn), 12205 (digitool), FADT12205 (IID), fau:9112 (fedora)
Note(s): Thesis (Ph.D.)--Florida Atlantic University, 2006.
Subject(s): Terpenes--Synthesis
Marine pharmacology
Alcyonacea
Biosynthesis
Anti-inflammatory agents
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/12205
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
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Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.