You are here
Strategies for flow cytometric analysis of marine microalgae and sponge cells
| Title: | Strategies for flow cytometric analysis of marine microalgae and sponge cells. |
 97 views 29 downloads |
|---|---|---|
| Name(s): |
Yentsch, C. M., creator Pomponi, Shirley A., creator |
|
| Type of Resource: | text | |
| Genre: | Article | |
| Issuance: | single unit | |
| Date Issued: | 1994 | |
| Publisher: | Academic Press, Inc. | |
| Extent: | 17 p. | |
| Physical Description: | ||
| Language(s): | English | |
| Identifier: | 3353886 (digitool), FADT3353886 (IID), fau:6663 (fedora), 10.1016/S0091-679X(08)61093-6 (doi) | |
| Note(s): |
The objective of this chapter is to detail two strategies important in order to overcome constraints inherent in flow cytometric analysis of marine microalgae and sponge cells. The two strategies are: (I) selective cell enrichment, employing physical separation techniques, and (2) selective signal enhancement, optimizing the optics. Specifically, we detail cell dissociation and density gradient separation of sponge cells, concentration of microalgae from seawater samples, determination of absolute (vs relative) abundance of microalgal subpopulations, and a metabolic index protocol that can be used for both microalgae and sponge cells. The final published version of this manuscript is available at http://www.sciencedirect.com/science/bookseries/0091679X and may be cited as: Yentsch, C. M., & Pomponi, S. A. (1994). Strategies for flow cytometric analysis of marine microalgae and sponge cells. In Z. Darzynkiewicz, J. P. Robinson & H. A. Crissman (eds.). Methods in Cell Biology, Part B, 42, 523-538. doi:10.1016/S0091-679X(08)61093-6 Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1039. |
|
| Subject(s): |
Microalgae Sponges Cytometric cellular analysis |
|
| Persistent Link to This Record: | http://purl.flvc.org/FCLA/DT/3353886 | |
| Links: | http://dx.doi.org/10.1016/S0091-679X(08)61093-6 | |
| Restrictions on Access: | ©1994 Academic Press, Inc. | |
| Host Institution: | FAU |
