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Recovery of functional human lymphocytes from Leukotrap* filters
| Title: | Recovery of functional human lymphocytes from Leukotrap* filters. |
 96 views 32 downloads |
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| Name(s): |
Longley, Ross E., creator Stewart, D., creator Harbor Branch Oceanographic Institute |
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| Type of Resource: | text | |
| Genre: | Article | |
| Issuance: | single unit | |
| Date Issued: | 1989 | |
| Publisher: | Elsevier B.V. | |
| Extent: | 7 p. | |
| Physical Description: | ||
| Language(s): | English | |
| Identifier: | 3353830 (digitool), FADT3353830 (IID), fau:6635 (fedora), 10.1016/0022-1759(89)90416-X (doi) | |
| Note(s): |
We report here a method by which functional human PBL may be recovered from a blood product preparation unit (Leukotrap) which is normally employed by blood banks to prepare a leukocyte-poor blood product and which is commonly discarded after use. Blood filters which were back-flushed with cold, phosphate-buffered saline (PBS) and the resulting buffy coats collected and separated on Histopaque gradients yielded an average of 2.7 × 108 (±1.17 × 108) cells per filter processed (n = 38), with viability greater than 95%. The final published version of this manuscript is available at http://www.sciencedirect.com/science/journal/00221759 and may be cited as: Longley, R. E., & Stewart, D. (1989). Recovery of functional human lymphocytes from Leukotrap filters. Journal of Immunological Methods, 121(2), 33-38.doi:10.1016/0022-1759(89)90416-X Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #692. |
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| Subject(s): |
Lymphocytes Leucocytes Blood--Filtration |
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| Persistent Link to This Record: | http://purl.flvc.org/FCLA/DT/3353830 | |
| Links: | http://dx.doi.org/10.1016/0022-1759(89)90416-X | |
| Restrictions on Access: | ©1989 Elsevier B.V. | |
| Host Institution: | FAU |
