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A rapid colorimetric microassay to detect agonists/antagonists of protein kinase C based on adherence of EL-4.IL-2 cells

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Date Issued:
1991
Title: A rapid colorimetric microassay to detect agonists/antagonists of protein kinase C based on adherence of EL-4.IL-2 cells.
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Name(s): Longley, Ross E., creator
Harmody, Dedra K., creator
Harbor Branch Oceanographic Institute
Type of Resource: text
Genre: Article
Issuance: single unit
Date Issued: 1991
Publisher: Nature Publishing Group
Extent: 11 p.
Physical Description: pdf
Language(s): English
Identifier: 3351944 (digitool), FADT3351944 (IID), fau:6508 (fedora)
Note(s): A rapid, colorimetric, microassay for detection of agents which are known agonists/antagonists of protein kinase C (PKC) was developed, utilizing their effects on adherence of EL-4.IL-2 cells. Cells that were incubated with agents which are known inducers of PKC activation, phorbol 12-myristate 13-acetate (PMA), phorbol 12, 13-dibutyrate (PDBu), mezerein and indolactam V, readily adhered to wells of 96 well microtiter plates within 1 - 2 hours, whereas cells incubated with the negative PKC activator, 4α-phorbol 12-myristate 13-acetate (4α-PMA), which is structurally related to PMA (4β-PMA), did not adhere.
This manuscript is an author version with the final publication available at http://www.nature.com/ja/index.html and may be cited as: Longley R. E., & Harmody, D. (1991). A rapid colorimetric microassay to detect agonists/antagonists of protein kinase C based on adherence of EL-4.IL-2 cells. The Journal of Antibiotics, 44(1), 93-102.
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #818.
Subject(s): Protein kinase C
Colorimetric analysis
Diglycerides
Persistent Link to This Record: http://purl.flvc.org/FCLA/DT/3351944
Restrictions on Access: ©1991 Nature Publishing Group
Host Institution: FAU