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Separation and concentration of phytoplankton populations using centrifugal elutriation
- Date Issued:
- 1989
Title: | Separation and concentration of phytoplankton populations using centrifugal elutriation. |
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Name(s): |
Pomponi, Shirley A., creator Cucci, T. L., creator |
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Type of Resource: | text | |
Genre: | Article | |
Issuance: | single unit | |
Date Issued: | 1989 | |
Publisher: | John Wiley & Sons, Inc. | |
Extent: | 8 p. | |
Physical Description: | ||
Language(s): | English | |
Identifier: | 3342295 (digitool), FADT3342295 (IID), fau:6407 (fedora), 10.1002/cyto.990100513 (doi) | |
Note(s): |
Unialgal cultures, mixtures of two phytoplankton cultures, and natural seawater samples were elutriated to determine the feasibility of this technique for collecting fractions of different cell cycle phases, separating two phytoplankton species, and concentrating cells from dilute samples. Elutriation resulted in the separation of a culture of Dunaliella tertiolecta and Phaeodactylum tricornutum into homogeneous fractions of each species. Cells in the natural seawater sample were concentrated by nearly 2 orders of magnitude. Centrifugal elutriation provides an alternative cell separation and concentration technique when large numbers of cells are required. This manuscript is an author version with the final publication available at http://www.wiley.com/WileyCDA/ and may be cited as: Pomponi, S. A., & Cucci, T. L. (1989). Separation and concentration of phytoplankton populations using centrifugal elutriation. Cytometry, 10(5), 580-586. doi:10.1002/cyto.990100513 Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #728. |
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Subject(s): |
Phytoplankton populations Flow cytometry Cell separation |
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Persistent Link to This Record: | http://purl.flvc.org/FCLA/DT/3342295 | |
Links: | http://dx.doi.org/10.1002/cyto.990100513 | |
Restrictions on Access: | ©1989 John Wiley & Sons, Inc. | |
Host Institution: | FAU |