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A mammalian cell agar-diffusion assay for the detection of toxic compounds
| Title: | A mammalian cell agar-diffusion assay for the detection of toxic compounds. |
 89 views 13 downloads |
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| Name(s): |
Burres, N. S., creator Hunter, J. E., creator Wright, Amy E., creator |
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| Type of Resource: | text | |
| Genre: | Article | |
| Issuance: | single unit | |
| Date Issued: | 1989 | |
| Publisher: | The American Chemical Society and American Society of Pharmacognosy | |
| Extent: | 7 p. | |
| Physical Description: | ||
| Language(s): | English | |
| Identifier: | 3332955 (digitool), FADT3332955 (IID), fau:6180 (fedora), 10.1021/np50063a010 (doi) | |
| Note(s): |
A method using murine P-388 leukemia or human HT-29 colon carcinoma cells was developed for the bioautography of potential antitumor agents. Of 18 cancer chemotherapeutic drugs and natural products tested, all were detected by toxicity at 0.01 or 1.0 µg with P-388 cells, and 11 of the 18 were detected at 10 µg or less with HT-29 cells. Bioautography of a crude extract of Pseudoplexaura wagenaari and subsequent purification yielded the known compound crassin acetate. With modification, the assay detected specifically toxic DNA-binding agents. This document is the accepted manuscript version of a published work that appeared in final form in Journal of Natural Products after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/np50063a010. This article may be cited as: Burress, N. S., Hunter, J. E., & Wright, A. E. (1989). A mammalian cell agar-diffusion assay for the detection of toxic compounds. Journal of Natural Products, 52(3), 522-527. doi:10.1021/np50063a010 Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #694. |
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| Subject(s): |
Antineoplastic agents Toxins Chromatographic analysis Thin layer chromatography |
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| Persistent Link to This Record: | http://purl.flvc.org/FCLA/DT/3332955 | |
| Links: | http://dx.doi.org/10.1021/np50063a010 | |
| Restrictions on Access: | ©1989 The American Chemical Society and American Society of Pharmacognosy | |
| Host Institution: | FAU |
