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Induction of apoptosis in mouse thymocytes by Microcolin A and its synthetic analog

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Date Issued:
1999
Title: Induction of apoptosis in mouse thymocytes by Microcolin A and its synthetic analog.
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Name(s): Zhang, L.-H., creator
Longley, Ross E., creator
Harbor Branch Oceanographic Institute
Type of Resource: text
Genre: Article
Issuance: single unit
Date Issued: 1999
Publisher: Elsevier B.V.
Extent: 17 p.
Physical Description: pdf
Language(s): English
Identifier: 3331937 (digitool), FADT3331937 (IID), fau:6163 (fedora), 10.1016/S0024-3205(99)00028-4 (doi)
Note(s): Microcolin A (Mic-1), a marine-derived compound, has been shown to be a novel antiproliferative and immunosuppressive agent. We investigated the ability of Mic-1 and its chemosynthetic analog, microcolin A3 (Mic-3), to induce apoptosis in murine thymocytes. Following incubation of the cells with Mic-1 (10–100 nM) or Mic-3 (10–100 nM), internucleosomal DNA fragmentation in apoptotic cells was detected by agarose gel electrophoresis and the diphenylamine (DPA) assay; the presence of hypodiploid nuclei assessed by propidium iodide (PI) staining; and the percentages of apoptotic and necrotic cells quantified by morphological observation and fluorescein labeled annexin-V binding.
This manuscript is an author version with the final publication available at http://www.sciencedirect.com/science/journal/00243205 and may be cited as: Zhang, L.-H., & Longley, R. E. (1999). Induction of apoptosis in mouse thymocytes by Microcolin A and its synthetic analog. Life Sciences, 64(12), 1013-1028. doi:10.1016/S0024-3205(99)00028-4
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1268.
Subject(s): Apoptosis
Thymocytes
Persistent Link to This Record: http://purl.flvc.org/FCLA/DT/3331937
Links: http://dx.doi.org/10.1016/S0024-3205(99)00028-4
Restrictions on Access: ©1999 Elsevier B.V.
Host Institution: FAU