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Induction of apoptosis in mouse thymocytes by Microcolin A and its synthetic analog
| Title: | Induction of apoptosis in mouse thymocytes by Microcolin A and its synthetic analog. |
 68 views 14 downloads |
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| Name(s): |
Zhang, L.-H., creator Longley, Ross E., creator Harbor Branch Oceanographic Institute |
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| Type of Resource: | text | |
| Genre: | Article | |
| Issuance: | single unit | |
| Date Issued: | 1999 | |
| Publisher: | Elsevier B.V. | |
| Extent: | 17 p. | |
| Physical Description: | ||
| Language(s): | English | |
| Identifier: | 3331937 (digitool), FADT3331937 (IID), fau:6163 (fedora), 10.1016/S0024-3205(99)00028-4 (doi) | |
| Note(s): |
Microcolin A (Mic-1), a marine-derived compound, has been shown to be a novel antiproliferative and immunosuppressive agent. We investigated the ability of Mic-1 and its chemosynthetic analog, microcolin A3 (Mic-3), to induce apoptosis in murine thymocytes. Following incubation of the cells with Mic-1 (10–100 nM) or Mic-3 (10–100 nM), internucleosomal DNA fragmentation in apoptotic cells was detected by agarose gel electrophoresis and the diphenylamine (DPA) assay; the presence of hypodiploid nuclei assessed by propidium iodide (PI) staining; and the percentages of apoptotic and necrotic cells quantified by morphological observation and fluorescein labeled annexin-V binding. This manuscript is an author version with the final publication available at http://www.sciencedirect.com/science/journal/00243205 and may be cited as: Zhang, L.-H., & Longley, R. E. (1999). Induction of apoptosis in mouse thymocytes by Microcolin A and its synthetic analog. Life Sciences, 64(12), 1013-1028. doi:10.1016/S0024-3205(99)00028-4 Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1268. |
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| Subject(s): |
Apoptosis Thymocytes |
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| Persistent Link to This Record: | http://purl.flvc.org/FCLA/DT/3331937 | |
| Links: | http://dx.doi.org/10.1016/S0024-3205(99)00028-4 | |
| Restrictions on Access: | ©1999 Elsevier B.V. | |
| Host Institution: | FAU |
