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Cell cycle analysis of primary sponge cell cultures
| Title: | Cell cycle analysis of primary sponge cell cultures. |
 76 views 12 downloads |
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| Name(s): |
Schippers, K. J., creator Martens, D. E.,, creator Pomponi, Shirley A., creator Wijffels, R. H., creator |
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| Type of Resource: | text | |
| Genre: | Article | |
| Issuance: | single unit | |
| Date Issued: | 2011 | |
| Publisher: | Springer | |
| Extent: | 11 p. | |
| Physical Description: | ||
| Language(s): | English | |
| Identifier: | 3318911 (digitool), FADT3318911 (IID), fau:6010 (fedora), 10.1007/s11626-011-9391-x (doi) | |
| Note(s): |
Proliferation of sponge cells is generally measured via cell counts or viability assays. However, more insight into the proliferative state of a sponge cell population can be obtained from the distribution of the cells over the different phases of the cell cycle. Cell cycle distribution of sponge cells was measured via flow cytometry after staining the DNA with propidium iodide. The five sponges studied in this paper all showed a large fraction of cells in G1/G0 compared to G2/M and S, indicating that cells were not actively dividing. This manuscript is an author version with the final publication available at http://www.springerlink.com and may be cited as: Schippers, K. J., Martens, D. E., Pomponi, S. A., & Wijffels, R. H. (2011). Cell cycle analysis of primary sponge cell cultures. In Vitro Cellular & Developmental Biology - Animal, 47(4), 302-311. doi:10.1007/s11626-011-9391-x Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1831. |
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| Subject(s): |
Sponges--Cytology Cell cycle Cell proliferation Flow cytometry |
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| Persistent Link to This Record: | http://purl.flvc.org/FCLA/DT/3318911 | |
| Links: | http://dx.doi.org/10.1007/s11626-011-9391-x | |
| Restrictions on Access: | ©2011 The Authors | |
| Host Institution: | FAU |
