You are here
Isolation and characterization of niphatevirin, a human-immunodeficiency-virus-inhibitory glycoprotein from the marine sponge Niphates erecta.
| Title: | Isolation and characterization of niphatevirin, a human-immunodeficiency-virus-inhibitory glycoprotein from the marine sponge Niphates erecta. |
 85 views 16 downloads |
|---|---|---|
| Name(s): |
O'Keefe, Barry R., creator Beutler, John A., creator Cardellina, John H., II, creator Gulakowski, Robert J., creator Krepps, Benjamin L., creator McMahon, James B., creator Sowder, Raymond C., II, creator Henderson, Louis E., creator Pannell, Lewis K., creator Pomponi, Shirley A., creator Boyd, Michael R., creator |
|
| Type of Resource: | text | |
| Genre: | Article | |
| Issuance: | single unit | |
| Date Issued: | 1997 | |
| Publisher: | John Wiley & Sons, Inc. | |
| Extent: | 8 p. | |
| Physical Description: | ||
| Language(s): | English | |
| Identifier: | 3174088 (digitool), FADT3174088 (IID), fau:5767 (fedora), 10.1111/j.1432-1033.1997.t01-1-00047.x (doi) | |
| Note(s): |
Anti-human immunodeficiency virus (HIV)-bioassay-guided fractionation of aqueous extracts of the Caribbean sponge Niphates erecta led to isolation of a novel anti-HIV protein, named niphatevirin. The protein was purified to homogeneity by ethanol precipitation, ammonium sulfate precipitation, gel-permeation chromatography and concanavalin-A–Sepharose affinity chromatography. Niphatevirin potently inhibited the cytopathic effects of HIV-1 infection in cultured human lymphoblastoid (CEM-SS) cells; the effective concentration of drug that results in 50% protection of the cells through inhibition of cell lethality, cell-cell fusion and syncytium formation was approximately 10 nM. This manuscript is an author version with the final publication available at http://www.wiley.com/WileyCDA/ and may be cited as: O’Keefe, B. R., Beutler, J. A., Cardellina II, J. H., Gulakowski, R. J., Krepps, B. L., McMahon, J. B., Sowder II, R. C., Henderson, L. E., Pannell, L. K., Pomponi, S. A., & Boyd, M. R. (1997). Isolation and characterization of niphatevirin, a human-immunodeficiency-virus-inhibitory glycoprotein from the marine sponge Niphates erecta. European Journal of Biochemistry, 245(1), 47-53. doi:10.1111/j.1432-1033.1997.t01-1-00047.x Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1196. |
|
| Subject(s): |
Sponges Sponges--Anatomy |
|
| Persistent Link to This Record: | http://purl.flvc.org/FCLA/DT/3174088 | |
| Links: | http://dx.doi.org/10.1111/j.1432-1033.1997.t01-1-00047.x | |
| Restrictions on Access: | ©1997 Federation of European Biochemical Societies | |
| Host Institution: | FAU |
