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Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis

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Date Issued:
2005
Title: Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis.
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Name(s): Snyder, Richard V., creator
Guerrero, Maria A., creator
Sinigalliano, Christopher D., creator
Winshell, Jamie, creator
Perez, Roberto, creator
Lopez, Jose V., creator
Rein, Kathleen S., creator
Harbor Branch Oceanographic Institute
Type of Resource: text
Genre: Article
Issuance: single unit
Date Issued: 2005
Publisher: Elsevier Ltd.
Extent: 15 p.
Physical Description: pdf
Language(s): English
Identifier: 2827832 (digitool), FADT2827832 (IID), fau:5571 (fedora), 10.1016/j.phytochem.2005.06.010 (doi)
Note(s): The carbon framework of all polyketides is assembled by a polyketide synthase (PKS). Previously, PKS encoding genes were amplified from K. brevis culture and their similarity to a PKS gene from the closely related protist, Cryptosporidium parvum, suggested that these genes originate from the dinoflagellate. However, K. brevis has not been grown axenically. The associated bacteria might be the source of the toxins or the PKS genes. Herein we report the localization of PKS encoding genes by a combination of flow cytometry/PCR and fluorescence in situ hybridization (FISH).
This is the author’s version of a work accepted for publication byElsevier. Changes resulting from the publishing process, including peer review, editing,corrections, structural formatting and other quality control mechanisms, may not be reflectedin this document. Changes may have been made to this work since it was submitted forpublication. The definitive version has been published athttp://www.elsevier.com/locate/phytochem and may be cited as Snyder, Richard V., MariaA. Guerrero, Christopher D. Sinigalliano, Jamie Winshell, Roberto Perez, Jose V. Lopez,Kathleen S. Rein (2005) Localization of polyketide synthase encoding genes to the toxicdinoflagellate Karenia brevis, Phytochemistry 66: 1767-1780 doi:10.1016/j.phytochem.2005.06.010
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #s 1600 and 1607.
Subject(s): Polyketides
Dinoflagellates, Fossil --Identification
Seafood --Contamination
Seafood --Microbiology
Microbial ecology
Persistent Link to This Record: http://purl.flvc.org/FCLA/DT/2827832
Links: http://dx.doi.org/10.1016/j.phytochem.2005.06.010
Restrictions on Access: ©2005 Elsevier Ltd.
Host Institution: FAU
Other Version: This is the author’s version of a work accepted for publication by Elsevier. Changes resulting from the publishing process, including peer review, editing, corrections, structural formatting and other quality control mechanisms, may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. The definitive version has been published at http://www.elsevier.com/locate/phytochem and may be cited as Snyder, Richard V., Maria A. Guerrero, Christopher D. Sinigalliano, Jamie Winshell, Roberto Perez, Jose V. Lopez, Kathleen S. Rein (2005) Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis, Phytochemistry 66: 1767–1780 doi:10.1016/j.phytochem.2005.06.010.