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Quantitative assessment of marine sponge cells in vitro: Development of improved growth medium
| Title: | Quantitative assessment of marine sponge cells in vitro: Development of improved growth medium. |
 108 views 22 downloads |
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| Name(s): |
Willoughby, Robin, creator Pomponi, Shirley A., creator |
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| Type of Resource: | text | |
| Genre: | Article | |
| Issuance: | single unit | |
| Date Issued: | 2000 | |
| Publisher: | Society for In Vitro Biology | |
| Extent: | 8 p. | |
| Physical Description: | ||
| Language(s): | English | |
| Identifier: | 2795606 (digitool), FADT2795606 (IID), fau:5534 (fedora), 10.1290/1071-2690(2000)036<0194:QAOMSC>2.0.CO;2 (doi) | |
| Note(s): |
As sources of natural products with potential human therapeutic value, marine sponges are important subjects for cell culture studies. A critical component of any cell culture system is its growth medium. Proceeding from the hypotheses that the thawed, cryopreserved, primary cells would display detectable differential responses and that those responses could be comparatively quantified, this study has established that multiwell screening assays are useful tools for improving medium formulations in cell cultures of the marine sponge, Teichaxinella morchella. Fluorescent probe signals were correlated with known cell densities and viabilities in a 96-well format. Analysis of variance and post-test methods were applied to judge the significance of signal differences seen in a variety of medium formulations. Results from a series of experiments suggested that reducing glutamine and selenium concentrations in the standard medium would result in greater DNA, protein, and esterase activity signals. This manuscript is a version of an article with the final publication found online at http://www.springerlink.com and may be cited as: Willoughby, Robin and Shirley A. Pomponi (2000) Quantitative assessment of marine sponge cells in vitro: Development of improved growth medium, In Vitro Cellular & Developmental Biology -Animal 36:194-200 doi: 10.1290/1071-2690(2000)036<0194:QAOMSC>2.0.CO;2 Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1317. |
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| Subject(s): |
Animal cell biotechnology Marine biology Cell culture --Technique |
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| Persistent Link to This Record: | http://purl.flvc.org/FCLA/DT/2795606 | |
| Restrictions on Access: | ©2000 Society for In Vitro Biology. | |
| Host Institution: | FAU |
