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Improved recoverability of microbial colonies from marine sponge samples
| Title: | Improved recoverability of microbial colonies from marine sponge samples. |
 98 views 42 downloads |
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| Name(s): |
Olson, Julie B., creator Lord, C. C., creator McCarthy, Peter J., creator |
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| Type of Resource: | text | |
| Genre: | Article | |
| Issuance: | single unit | |
| Date Issued: | 2000 | |
| Publisher: | Springer-Verlag | |
| Extent: | 10 p. | |
| Physical Description: | ||
| Language(s): | English | |
| Identifier: | 2783209 (digitool), FADT2783209 (IID), fau:5514 (fedora), 10.1007/s002480000058 (doi) | |
| Note(s): |
The growth of microorganisms from marine sponge samples was studied on various low to high nutrient solid media using media supplements. The supplements utilized were catalase, sodium pyruvate, and a combination of the two. Medium composition was found to influence the growth response on the supplemented media. Microorganisms on low nutrient media responded more favorably to the media additions than on high nutrient media. Thirty-five percent of the supplemented media demonstrated colony forming unit (CFU) recoveries that were 50% or greater than those of the unamended control plates. Twenty-one percent showed recoveries of more than 100% of the control values, with sodium pyruvate additions providing for the greatest overall increase in recovery, whether alone or in conjunction with catalase. These findings suggest that addition of catalase or sodium pyruvate to solid growth and isolation media may improve recoverability of microorganisms from natural samples. This manuscript is a version of an article with the final publication found online at http://www.springerlink.com and may be cited as: Olson, J. B., C. C. Lord and P. J. McCarthy (2000) Improved Recoverability of Microbial Colonies from Marine Sponge Samples, Microbial Ecology 40:139–147 DOI: 10.1007/s002480000058 Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1360. |
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| Subject(s): |
Microbiology --Cultures and culture media Sponges |
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| Persistent Link to This Record: | http://purl.flvc.org/FCLA/DT/2783209 | |
| Links: | http://dx.doi.org/10.1007/s002480000058 | |
| Restrictions on Access: | ©2000 Springer-Verlag | |
| Host Institution: | FAU |
