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In vitro culture of the tropical sponge Axinella Corrugata (Demospongiae): effect of food cell concentration on growth, clearance rate, and biosynthesis of stevensine
- Date Issued:
- 2003
Title: | In vitro culture of the tropical sponge Axinella Corrugata (Demospongiae): effect of food cell concentration on growth, clearance rate, and biosynthesis of stevensine. |
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Name(s): |
Duckworth, Alan R., creator Samples, Gail A., creator Wright, Amy E., creator Pomponi, Shirley A., creator |
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Type of Resource: | text | |
Genre: | Article | |
Issuance: | single unit | |
Date Issued: | 2003 | |
Publisher: | Springer-Verlag | |
Extent: | 10 p. | |
Physical Description: | ||
Language(s): | English | |
Identifier: | 2709450 (digitool), FADT2709450 (IID), fau:5507 (fedora), 10.1007/s10126-002-0111-0 (doi) | |
Note(s): |
In vitro culture is one possible method for supplying sponge metabolites for pharmaceutical applications, but appropriate feeding regimens that maximize both growth and metabolite biosynthesis are largely unknown. According to the natural concentration (NC) of cells 1 to 50 lm in size that are available to wild Axinella corrugata, we fed explants a multispecific diet of bacteria, microalgae, and yeast at 4 different concentrations: 1NC, 3NC, 5NC, and 5+1NC (the last consisted of 5 NC of bacteria and 1 NC of microalgae and yeast). Explants fed a 3NC diet had the best culture response, growing on average from 8.5 g to 10.3 g in 8 weeks, and showing a 110% increase in concentration (milligrams per gram of dry weight) of the antitumor compound stevensine. This manuscript is a version of an article with the original publication available at http://www.springerlink.com and may be cited as: Duckworth, Alan R., Gail A. Samples, Amy E. Wright and Shirley A. Pomponi (2003) In Vitro CuIture of the Tropical Sponge Axinella corrugata (Demospongiae): Effect of Food Cell Concentration on Growth, Clearance Rate, and Biosynthesis of Stevensine, Marine Biotechnology 5:519–527 DOI: 10.1007/s10126-002-0111-0 Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1503. |
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Subject(s): | Sponges --Microbiology | |
Persistent Link to This Record: | http://purl.flvc.org/FCLA/DT/2709450 | |
Links: | http://dx.doi.org/10.1007/s10126-002-0111-0 | |
Restrictions on Access: | ©2003 Springer-Verlag. | |
Host Institution: | FAU |