You are here

FAU Collections » FAU Research Repository » FAU College Collections » Harriet L. Wilkes Honors College » Honors Student Theses

Isolating the Manganese Superoxide Dismutase Gene (SOD2) from T. scripta""

Download pdf | Full Screen View

Date Issued:
2011
Abstract/Description:
Reactive oxygen species (ROS) are molecular oxygen-derived molecules that are exceedingly reactive, often generated as free radical bi-products of mitochondrial respiration, which cause oxidative stress that leads to aging. To avoid the generation of ROS, aerobic organisms have antioxidant defense mechanisms that use enzymes such as Superoxide Dismutase (SOD) to convert the superoxide radicals into hydrogen peroxide and oxygen. Manganesecontaining SOD (Mn-SOD), a product of the SOD2 gene is found in all eukaryotic organisms in the mitochondrial matrix, including T. scripta (red-eared slider turtle). Extraordinarily, T. scripta can live without oxygen for long periods of time without experiencing adverse effects when oxygen is again available. The purpose of our experiment is to clone the T. scripta SOD2 gene, and test its activity. A partial fragment of the gene has been isolated by screening the cDNA library, and we are currently working on finding the remaining sequence.
Title: Isolating the Manganese Superoxide Dismutase Gene (SOD2) from T. scripta"".
31 views
13 downloads
Name(s): Sanchez, Jairo, author
Kirchman, Paul A., Thesis advisor
Chandrasekhar, Chitra , Thesis advisor
Harriet L. Wilkes Honors College
Florida Atlantic University
Type of Resource: text
Genre: Thesis
Date Created: 2011
Date Issued: 2011
Publisher: Florida Atlantic University Digital Library
Place of Publication: Boca Raton, Fla.
Physical Form: online resource
Extent: 31 p.
Language(s): English
Abstract/Description: Reactive oxygen species (ROS) are molecular oxygen-derived molecules that are exceedingly reactive, often generated as free radical bi-products of mitochondrial respiration, which cause oxidative stress that leads to aging. To avoid the generation of ROS, aerobic organisms have antioxidant defense mechanisms that use enzymes such as Superoxide Dismutase (SOD) to convert the superoxide radicals into hydrogen peroxide and oxygen. Manganesecontaining SOD (Mn-SOD), a product of the SOD2 gene is found in all eukaryotic organisms in the mitochondrial matrix, including T. scripta (red-eared slider turtle). Extraordinarily, T. scripta can live without oxygen for long periods of time without experiencing adverse effects when oxygen is again available. The purpose of our experiment is to clone the T. scripta SOD2 gene, and test its activity. A partial fragment of the gene has been isolated by screening the cDNA library, and we are currently working on finding the remaining sequence.
Identifier: FA00003605 (IID)
Degree granted: Thesis (B.A.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2011.
Collection: Florida Atlantic University Digital Library Collections
Note(s): Includes bibliography.
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00003605
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU

In Collections