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The Role of IL-17 in T-Cell Development

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Date Issued:
2011
Abstract/Description:
T-lymphocytes develop from bone marrow derived hematopoietic stem cells (HSCs) and mature in the thymus, where they participate in reciprocal signaling with thymic stromal cells. The thymic developmental stages are well characterized, but only a few intrathymic signals that influence the development of T-lymphocytes have been identified. Previous microarray experiments revealed interleukin-17A (IL-17A) and its receptor (IL-17RA) as a possible stromal-lymphoid signal. In this study, an IL-17RA-/- knockout was used to determine whether the IL-17RA gene has a role in T-lymphocyte maturation. We made competitive bone marrow chimeras and analyzed the percentage of donor wildtype and mutant HSCs present in the bone marrow, and compared it to the percentage of a particular blood cell type that developed from these donor HSCs. We found that IL-17RA influences the maturation of T-lymphocytes, but does not affect the development of other immune cells such as B-lymphocytes, macrophages, and granulocytes.
Title: The Role of IL-17 in T-Cell Development.
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Name(s): Gopaldas, Manesh, author
Kirchman, Paul A., Thesis advisor
Chandrasekhar, Chitra , Thesis advisor
Harriet L. Wilkes Honors College
Florida Atlantic University
Type of Resource: text
Genre: Thesis
Date Created: 2011
Date Issued: 2011
Publisher: Florida Atlantic University Digital Library
Place of Publication: Boca Raton, Fla.
Physical Form: online resource
Extent: 44 p.
Language(s): English
Abstract/Description: T-lymphocytes develop from bone marrow derived hematopoietic stem cells (HSCs) and mature in the thymus, where they participate in reciprocal signaling with thymic stromal cells. The thymic developmental stages are well characterized, but only a few intrathymic signals that influence the development of T-lymphocytes have been identified. Previous microarray experiments revealed interleukin-17A (IL-17A) and its receptor (IL-17RA) as a possible stromal-lymphoid signal. In this study, an IL-17RA-/- knockout was used to determine whether the IL-17RA gene has a role in T-lymphocyte maturation. We made competitive bone marrow chimeras and analyzed the percentage of donor wildtype and mutant HSCs present in the bone marrow, and compared it to the percentage of a particular blood cell type that developed from these donor HSCs. We found that IL-17RA influences the maturation of T-lymphocytes, but does not affect the development of other immune cells such as B-lymphocytes, macrophages, and granulocytes.
Identifier: FA00003578 (IID)
Degree granted: Thesis (B.A.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2011.
Collection: Florida Atlantic University Digital Library Collections
Note(s): Includes bibliography.
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00003578
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
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Host Institution: FAU

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