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Molecular and phenotypic characterization of MsrA MsrB mutants of Drosophila melanogaster
| Title: | Molecular and phenotypic characterization of MsrA MsrB mutants of Drosophila melanogaster. |
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| Name(s): |
Robbins, Kelli. Charles E. Schmidt College of Science Department of Biological Sciences |
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| Type of Resource: | text | |
| Genre: | Electronic Thesis Or Dissertation | |
| Date Issued: | 2009 | |
| Publisher: | Florida Atlantic University | |
| Physical Form: | electronic | |
| Extent: | ix, 104 p. : ill. (some col.) | |
| Language(s): | English | |
| Summary: | Aging is a multifactoral biological process of progressive and deleterious changes partially attributed to a build up of oxidatively damaged biomolecules resulting from attacks by free radicals. Methionine sulfoxide reductases (Msrs) are enzymes that repair oxidized methionine (Met) residues found in proteins. Oxidized Met produces two enantiomers, Met-S-(o) and Met-R-(o), reduced by MsrA and MsrB respectively. Unlike other model organisms, our MsrA null fly mutant did not display increased sensitivity to oxidative stress or shortened lifespan, suggesting that in Drosophila, having either a functional copy of either Msr is sufficient. Here, two Msr mutant types were phenotypically assayed against isogenic controls. Results suggest that only the loss of both MsrA and MsrB produces increased sensitivity to oxidative stress and shortened lifespan, while locomotor defects became more severe with the full Msr knockout fly. | |
| Identifier: | 477183594 (oclc), 359920 (digitool), FADT359920 (IID), fau:4231 (fedora) | |
| Note(s): |
by Kelli Robbins. Thesis (M.S.)--Florida Atlantic University, 2009. Includes bibliography. Electronic reproduction. Boca Raton, Fla., 2009. Mode of access: World Wide Web. |
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| Subject(s): |
Genetic regulation Oxidation-reduction reaction Proteins -- Chemical modification Aging -- Molecular aspects Mutation (Biology) Cell metabolism Mitochondrial DNA |
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| Persistent Link to This Record: | http://purl.flvc.org/FAU/359920 | |
| Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
| Host Institution: | FAU |
