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ENGINEERING CHANNELS IN POROUS CALCIUM PHOSPHATE BIOCERAMIC SCAFFOLDS FOR BONE TISSUE REGENERATION
| Title: | ENGINEERING CHANNELS IN POROUS CALCIUM PHOSPHATE BIOCERAMIC SCAFFOLDS FOR BONE TISSUE REGENERATION. |
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| Name(s): |
Wang, Xuesong , author Kang, Yunqing , Thesis advisor Florida Atlantic University, Degree grantor Department of Ocean and Mechanical Engineering College of Engineering and Computer Science |
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| Type of Resource: | text | |
| Genre: | Electronic Thesis Or Dissertation | |
| Date Created: | 2019 | |
| Date Issued: | 2019 | |
| Publisher: | Florida Atlantic University | |
| Place of Publication: | Boca Raton, Fla. | |
| Physical Form: | application/pdf | |
| Extent: | 97 p. | |
| Language(s): | English | |
| Abstract/Description: | Inadequate nutrition exchange and slow transportation in a porous scaffold often resulted in insufficient vasculature formation, which hindered rapid bone regeneration. In this study, interconnected porous beta-tricalcium phosphate (b-TCP) scaffolds with channeled geometry were fabricated. In vitro fluid transportation and degradation of the scaffolds were performed. Cell attachment, migration, proliferation, and differentiation were carried out under both static and dynamic culturing conditions. A computational simulation model and a series of immunofluorescent staining were implemented to understand the mechanism of cell behavior in respond to different scaffolds geometry. We then implanted scaffolds into rat critical-sized calvarial defects to further evaluate channels’ function on bone regeneration in vivo. Results showed that multiple channeled geometry significantly accelerated the release of Ca2+ and increased the fluid diffusion efficiency. Moreover, multiple channels promoted human umbilical vein endothelial cells (HUVECs) infiltration, migration, besides prominently promoted alkaline phosphatase (ALP) activity, and up-regulated osteogenic gene expression in human bone marrow mesenchymal stem cells (hBMSCs) at both static and dynamic culturing conditions in vitro. The expression of both cell migration related protein a5 and angiogenesis related protein CD31 were upregulated by multiple channels in HUVECs. And the expression of mechanosensing markers, focal adhesion kinase (FAK), polymeric filamentous actin (Factin), and Yes-associated protein-1 (YAP-1) were highly stimulated by multiple channels in hBMSCs. The in vivo implantation and characterization results demonstrated more bone formation inside multiple-channeled scaffolds compared to non-channeled scaffolds. Multiple channels accelerated collagen type I, Bone Sialoprotein (Bsp), Osteocalcin (OC) protein expression prominently. The angiogenesis related protein CD31 staining displayed longer and more vasculature structures on multiple-channeled scaffolds compared to nonchanneled scaffolds. Fluorescent images of the fluorochrome labeled samples exhibited considerably more mineral deposition on multiple-channeled scaffolds than non-channeled scaffolds. All the findings suggested that the addition of multiple channels in the porous b-TCP scaffold is very promising approach to promote vascularization and bone tissue regeneration. | |
| Identifier: | FA00013426 (IID) | |
| Degree granted: | Dissertation (Ph.D.)--Florida Atlantic University, 2019. | |
| Collection: | FAU Electronic Theses and Dissertations Collection | |
| Note(s): | Includes bibliography. | |
| Subject(s): |
Tissue Engineering Bone and Bones Bone regeneration Calcium Phosphates Biocompatible Materials |
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| Held by: | Florida Atlantic University Libraries | |
| Sublocation: | Digital Library | |
| Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00013426 | |
| Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
| Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
| Host Institution: | FAU | |
| Is Part of Series: | Florida Atlantic University Digital Library Collections. |
