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SUPPORTED ERYTHROCYTE MEMBRANES ON PIEZOELECTRIC SENSORS FOR STUDYING THE INTERACTIONS BETWEEN NANOPARTICLES AND SURFACES OF RED BLOOD CELLS
| Title: | SUPPORTED ERYTHROCYTE MEMBRANES ON PIEZOELECTRIC SENSORS FOR STUDYING THE INTERACTIONS BETWEEN NANOPARTICLES AND SURFACES OF RED BLOOD CELLS. |
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| Name(s): |
Islam, Tanaz, author Yi, Peng, Thesis advisor Florida Atlantic University, Degree grantor Department of Civil, Environmental and Geomatics Engineering College of Engineering and Computer Science |
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| Type of Resource: | text | |
| Genre: | Electronic Thesis Or Dissertation | |
| Date Created: | 2019 | |
| Date Issued: | 2019 | |
| Publisher: | Florida Atlantic University | |
| Place of Publication: | Boca Raton, Fla. | |
| Physical Form: | application/pdf | |
| Extent: | 86 p. | |
| Language(s): | English | |
| Abstract/Description: | The Supported red blood cell membrane (SRBCm) was developed on a piezoelectric sensor to study the attachment of nanoparticles to erythrocyte surfaces. A well-dispersed colloidal suspension of fragments of RBCm was prepared from whole blood, and characterized thoroughly using cryogenic transmission electron microscopy, dynamic light scattering, and zeta potential analysis. To develop SRBCm, RBCm fragments were immobilized onthe sensor in a quartz crystal microbalance with dissipation monitoring system. A complete monolayer of flattened fragments of RBCm was formed on the positively charged surface of the piezoelectric sensor in 1 mM NaCl and 0.2 mM NaHCO3 at pH 7.1. The surface morphology of SRBCm was characterized via atomic force microscopy. The even distribution of surface proteins expressed on erythrocytes was found on SRBCm through indirect immunofluorescence microscopy. The attachment efficiencies of model nanoparticles, e.g. hematite nanoparticles and carboxylated polystyrene nanoparticles, on the SRBCm were quantified using a classic methodology. KEYWORDS: Supported erythrocyte membrane, piezoelectric sensor, phospholipid bilayers, nanoparticles | |
| Identifier: | FA00013383 (IID) | |
| Degree granted: | Thesis (M.S.)--Florida Atlantic University, 2019. | |
| Collection: | FAU Electronic Theses and Dissertations Collection | |
| Note(s): | Includes bibliography. | |
| Subject(s): |
Erythrocyte Membrane Piezoelectric polymer biosensors Nanoparticles Phospholipid bilayers Bilayer lipid membranes |
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| Held by: | Florida Atlantic University Libraries | |
| Sublocation: | Digital Library | |
| Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00013383 | |
| Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
| Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
| Host Institution: | FAU | |
| Is Part of Series: | Florida Atlantic University Digital Library Collections. |
