You are here

Neuroprotection from induced glutamate excitotoxicity by Conus brunneus conopeptides in a stroke-related model

Download pdf | Full Screen View

Date Issued:
2013
Summary:
Cone snails are carnivorous marine mollusks, utilizing their neuropeptide-rich venom for prey capture. The venom of Conus brunneus, a wide-spread Eastern Pacific vermivore, has not been extensively studied. In the current work, peptides from the dissected venom were characterized and tested using preliminary bioassays. Six peptides (A-F) were isolated and tested. Three peptide identities were determined by comparison with previously reported data: bru9a (A), bru3a (F), and an a-conotoxin (E). Preliminary screening in a stroke-related model of induced glutamate excitotoxicity in primary neuronal cells and PC12 cell cultures indicated potential neuroprotective activity of peptide fractions A, D, and F. Further testing is necessary to determine and verify structure, activity, target, and mechanism of action of the promising peptides from C. brunneus, which may prove effective neuropharmacological agents to treat stroke.
Title: Neuroprotection from induced glutamate excitotoxicity by Conus brunneus conopeptides in a stroke-related model.
189 views
79 downloads
Name(s): Crouch, Rebecca A.
Charles E. Schmidt College of Science
Department of Chemistry and Biochemistry
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Date Issued: 2013
Publisher: Florida Atlantic University
Physical Form: electronic
Extent: xiii, 85 p. : ill. (some col.)
Language(s): English
Summary: Cone snails are carnivorous marine mollusks, utilizing their neuropeptide-rich venom for prey capture. The venom of Conus brunneus, a wide-spread Eastern Pacific vermivore, has not been extensively studied. In the current work, peptides from the dissected venom were characterized and tested using preliminary bioassays. Six peptides (A-F) were isolated and tested. Three peptide identities were determined by comparison with previously reported data: bru9a (A), bru3a (F), and an a-conotoxin (E). Preliminary screening in a stroke-related model of induced glutamate excitotoxicity in primary neuronal cells and PC12 cell cultures indicated potential neuroprotective activity of peptide fractions A, D, and F. Further testing is necessary to determine and verify structure, activity, target, and mechanism of action of the promising peptides from C. brunneus, which may prove effective neuropharmacological agents to treat stroke.
Identifier: 858866188 (oclc), 3362331 (digitool), FADT3362331 (IID), fau:4169 (fedora)
Note(s): by Rebecca A. Crouch.
Thesis (M.S.)--Florida Atlantic University, 2013.
Includes bibliography.
Mode of access: World Wide Web.
System requirements: Adobe Reader.
Subject(s): Gastropoda -- Venom -- Therapeutic use
Peptides -- Structure
Neuroprotective agents
Central nervous system -- Diseases -- Treatment
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/3362331
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU