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Analysis of Expression and Subcellular Localization of OGT-1 in C. elegans

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Date Issued:
2018
Abstract/Description:
EEL-1, the HUWE1 ortholog in C. elegans, is a large E3 ubiquitin ligase that is involved in the regulation of neuronal function and development. Huwe1 has been shown to be associated with intellectual disability. Through the use of electrophysiology and cell specific rescue it has been shown that EEL-1 is involved in GABAergic synaptic transmission. The mechanism of action of EEL-1 within GABA neurons remains largely unknown. Affinity purification proteomics have identified OGT-1, an ortholog of mammalian O-linked N-acetylglucosamine (O-GlcNAc) transferase (Ogt), as an EEL-1 binding protein. By analyzing the expression pattern of OGT-1 in transgenic animals, we show that OGT-1 is expressed both GABAergic motor neurons and cholinergic motor neurons. Analysis of the subcellular distribution within GABAergic motor neurons shows that OGT-1 is enriched in presynaptic terminals. These findings support the hypothesis that EEL-1 and OGT-1 form a complex within GABA motor neurons to regulate synaptic transmission.
Title: Analysis of Expression and Subcellular Localization of OGT-1 in C. elegans.
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Name(s): Tavora, Rubens, author
Grill, Brock, Thesis advisor
Florida Atlantic University, Degree Grantor
Harriet L. Wilkes Honors College
Type of Resource: text
Genre: Thesis
Date Created: 2018
Date Issued: 2018
Publisher: Florida Atlantic University
Place of Publication: Jupiter, Florida
Physical Form: application/pdf
Extent: 22 p.
Language(s): English
Abstract/Description: EEL-1, the HUWE1 ortholog in C. elegans, is a large E3 ubiquitin ligase that is involved in the regulation of neuronal function and development. Huwe1 has been shown to be associated with intellectual disability. Through the use of electrophysiology and cell specific rescue it has been shown that EEL-1 is involved in GABAergic synaptic transmission. The mechanism of action of EEL-1 within GABA neurons remains largely unknown. Affinity purification proteomics have identified OGT-1, an ortholog of mammalian O-linked N-acetylglucosamine (O-GlcNAc) transferase (Ogt), as an EEL-1 binding protein. By analyzing the expression pattern of OGT-1 in transgenic animals, we show that OGT-1 is expressed both GABAergic motor neurons and cholinergic motor neurons. Analysis of the subcellular distribution within GABAergic motor neurons shows that OGT-1 is enriched in presynaptic terminals. These findings support the hypothesis that EEL-1 and OGT-1 form a complex within GABA motor neurons to regulate synaptic transmission.
Identifier: FAUHT00048 (IID)
Degree granted: Thesis (B.A.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2018.
Collection: FAU Honors Theses Digital Collection
Note(s): Includes bibliography.
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FAUHT00048
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.

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