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Development of A Portable Impedance Based Flow Cytometer for Diagnosis of Sickle Cell Disease

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Date Issued:
2018
Abstract/Description:
Sickle cell disease is an inherited blood cell disorder that affects about 100,000 people in the US and results in high cost of medical care exceeding $1.1 billion annually. Sickle cell patients suffer from unpredictable, painful vaso-occlusive crises. Portable, costeffective approaches for diagnosis and monitoring sickle blood activities are important for a better management of the disease and reducing the medical cost. In this research, a mobile application controlled, impedance-based flow cytometer is developed for the diagnosis of sickle cell disease. Calibration of the portable device is performed using a component of known impedance value. The preliminary test results are then compared to those obtained by a commercial benchtop impedance analyzer for further validation. With the developed portable flow cytometer, experiments are performed on two sickle cell samples and a healthy cell sample. The acquired results are subsequently analyzed with MATLAB scripts to extract single-cell level impedance information as well as statistics of different cell conditions. Significant differences in cell impedance signals are observed between sickle cells and normal cells, as well as between sickle cells under hypoxia and normoxia conditions.
Title: Development of A Portable Impedance Based Flow Cytometer for Diagnosis of Sickle Cell Disease.
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Name(s): Dieujuste, Darryl, author
Zhuang, Hanqi, Thesis advisor
Du, Sarah, Thesis advisor
Florida Atlantic University, Degree grantor
College of Engineering and Computer Science
Department of Computer and Electrical Engineering and Computer Science
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Date Created: 2018
Date Issued: 2018
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 57 p.
Language(s): English
Abstract/Description: Sickle cell disease is an inherited blood cell disorder that affects about 100,000 people in the US and results in high cost of medical care exceeding $1.1 billion annually. Sickle cell patients suffer from unpredictable, painful vaso-occlusive crises. Portable, costeffective approaches for diagnosis and monitoring sickle blood activities are important for a better management of the disease and reducing the medical cost. In this research, a mobile application controlled, impedance-based flow cytometer is developed for the diagnosis of sickle cell disease. Calibration of the portable device is performed using a component of known impedance value. The preliminary test results are then compared to those obtained by a commercial benchtop impedance analyzer for further validation. With the developed portable flow cytometer, experiments are performed on two sickle cell samples and a healthy cell sample. The acquired results are subsequently analyzed with MATLAB scripts to extract single-cell level impedance information as well as statistics of different cell conditions. Significant differences in cell impedance signals are observed between sickle cells and normal cells, as well as between sickle cells under hypoxia and normoxia conditions.
Identifier: FA00013145 (IID)
Degree granted: Thesis (M.S.)--Florida Atlantic University, 2018.
Collection: FAU Electronic Theses and Dissertations Collection
Note(s): Includes bibliography.
Subject(s): Sickle cell disease
Sickle cell anemia--Diagnosis
Flow cytometry--Diagnostic use
Mobile Applications
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00013145
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.