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OPTIMIZATION OF STABLE CELL-BASED OVEREXPRESSION OF MATRIX METALLOPROTEINASE 14

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Date Issued:
2017
Summary:
Matrix Metalloproteinase-14 (MMP-14) functions as a protease that assists in the digestion of type 1 collagen, a significant component of the extracellular matrix (ECM). MMP-14 may be essential in the metastases and invasion of cancers like lung, melanoma, and colon as well as diseases of the connective tissue. The progression of certain cancers may be constricted by developing specific enzymatic inhibitors that can target the action of this enzyme. In order to study the role of MMP-14 mediated invasion, we focused on the creation of a human breast adenocarcinoma (MCF-7) cell line stably overexpressing the MMP-14 protein. This cell line will be used for future diagnostic research of cell-based MMP-14 kinetic activity in order to eventually develop an effective and specific inhibitor for this protease.
Title: OPTIMIZATION OF STABLE CELL-BASED OVEREXPRESSION OF MATRIX METALLOPROTEINASE 14.
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Name(s): Estrada, Christie-Anne, author
Fields, Gregg B., Thesis advisor
Florida Atlantic University, Degree Grantor
Harriet L. Wilkes Honors College
Type of Resource: text
Genre: Thesis
Date Created: 2017
Date Issued: 2017
Publisher: Florida Atlantic University
Place of Publication: Jupiter, Florida
Physical Form: application/pdf
Extent: 30 p.
Language(s): English
Summary: Matrix Metalloproteinase-14 (MMP-14) functions as a protease that assists in the digestion of type 1 collagen, a significant component of the extracellular matrix (ECM). MMP-14 may be essential in the metastases and invasion of cancers like lung, melanoma, and colon as well as diseases of the connective tissue. The progression of certain cancers may be constricted by developing specific enzymatic inhibitors that can target the action of this enzyme. In order to study the role of MMP-14 mediated invasion, we focused on the creation of a human breast adenocarcinoma (MCF-7) cell line stably overexpressing the MMP-14 protein. This cell line will be used for future diagnostic research of cell-based MMP-14 kinetic activity in order to eventually develop an effective and specific inhibitor for this protease.
Identifier: FA00012616 (IID)
Degree granted: Thesis (B.A.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2017.
Collection: FAU Honors Theses Digital Collection
Note(s): Includes bibliography.
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00012616
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.

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