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INVASION ASSAY OF MELANOMA CELLS WM115 AND WM266-4

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Date Issued:
2017
Summary:
Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that play an important role in tumor growth and invasion (Gialeli, 2010). They can degrade cell-adhesion molecules that allow for cell-cell and cell-extracellular matrix interactions, which is necessary for cancer cells to degrade physical barriers (Gialeli, 2010). The presence of MMPs in cancer cells help promote cell invasion. In this project, melanoma cell lines WM115 (primary) and WM266-4 (metastatic) were used to conduct the invasion assay. The invasion assay was designed and optimized to test the ability of the cell to cut and pass through the collagen membrane. Next, a general MMP inhibitor was tested for inhibition of invasion. Varying inhibitor concentrations were used to see the effects. Following that, a more specific inhibitor targeting MT-1 MMP was tested with WM266-4 cells and inhibition of invasion was observed. MT1-MMP was the target of the inhibitor because of its involvement in cell invasion and its ability to cleave the collagen membrane.
Title: INVASION ASSAY OF MELANOMA CELLS WM115 AND WM266-4.
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Name(s): Davis, Dominique, author
Fields, Gregg B., Thesis advisor
Florida Atlantic University, Degree Grantor
Harriet L. Wilkes Honors College
Type of Resource: text
Genre: Thesis
Date Created: 2017
Date Issued: 2017
Publisher: Florida Atlantic University
Place of Publication: Jupiter, Florida
Physical Form: application/pdf
Extent: 22 p.
Language(s): English
Summary: Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that play an important role in tumor growth and invasion (Gialeli, 2010). They can degrade cell-adhesion molecules that allow for cell-cell and cell-extracellular matrix interactions, which is necessary for cancer cells to degrade physical barriers (Gialeli, 2010). The presence of MMPs in cancer cells help promote cell invasion. In this project, melanoma cell lines WM115 (primary) and WM266-4 (metastatic) were used to conduct the invasion assay. The invasion assay was designed and optimized to test the ability of the cell to cut and pass through the collagen membrane. Next, a general MMP inhibitor was tested for inhibition of invasion. Varying inhibitor concentrations were used to see the effects. Following that, a more specific inhibitor targeting MT-1 MMP was tested with WM266-4 cells and inhibition of invasion was observed. MT1-MMP was the target of the inhibitor because of its involvement in cell invasion and its ability to cleave the collagen membrane.
Identifier: FA00012615 (IID)
Degree granted: Thesis (B.A.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2017.
Collection: FAU Honors Theses Digital Collection
Note(s): Includes bibliography.
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00012615
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.

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