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CELLULAR SENESCENCE INCREASES IN THE ERCC1-/Δ MOUSE MODEL OF ACCELERATED AGING
- Date Issued:
- 2017
- Abstract/Description:
- Aging is the result of the progressive and intrinsic accumulation of detrimental changes in an organism over time. Understanding the molecular pathways that contribute to aging is critical for the development of therapeutic agents to treat age-related disorders. As an animal ages, it accumulates senescent cells, cells that are unable to grow or divide but remain metabolically active. They secrete Senescence Associated Secretory Phenotype (SASP) factors, which can disrupt tissue and cause age related diseases. The Ercc1-/Δ mice are an accelerated aging, progeriod model, and thus it was hypothesized that cellular senescence would increase in the Ercc1-/Δ mice. Through quantitative polymerase chain reaction (qPCR) analysis, the expression of senescence biomarkers p16INK4a, p21, IL-6 and TNF-α were measured to confirm that Ercc1-/Δ mice do experience an increase in cellular senescence. Additionally, we were able to determine that there are gender differences regarding the accumulation of senescent cells in various body parts.
Title: | CELLULAR SENESCENCE INCREASES IN THE ERCC1-/Δ MOUSE MODEL OF ACCELERATED AGING. |
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Name(s): |
Bukata, Christina , author Wetterer, James K., Thesis advisor Florida Atlantic University, Degree Grantor Harriet L. Wilkes Honors College |
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Type of Resource: | text | |
Genre: | Thesis | |
Date Created: | 2017 | |
Date Issued: | 2017 | |
Publisher: | Florida Atlantic University | |
Place of Publication: | Jupiter, Florida | |
Physical Form: | application/pdf | |
Extent: | 38 p. | |
Language(s): | English | |
Abstract/Description: | Aging is the result of the progressive and intrinsic accumulation of detrimental changes in an organism over time. Understanding the molecular pathways that contribute to aging is critical for the development of therapeutic agents to treat age-related disorders. As an animal ages, it accumulates senescent cells, cells that are unable to grow or divide but remain metabolically active. They secrete Senescence Associated Secretory Phenotype (SASP) factors, which can disrupt tissue and cause age related diseases. The Ercc1-/Δ mice are an accelerated aging, progeriod model, and thus it was hypothesized that cellular senescence would increase in the Ercc1-/Δ mice. Through quantitative polymerase chain reaction (qPCR) analysis, the expression of senescence biomarkers p16INK4a, p21, IL-6 and TNF-α were measured to confirm that Ercc1-/Δ mice do experience an increase in cellular senescence. Additionally, we were able to determine that there are gender differences regarding the accumulation of senescent cells in various body parts. | |
Identifier: | FA00012609 (IID) | |
Degree granted: | Thesis (B.A.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2017. | |
Collection: | FAU Honors Theses Digital Collection | |
Note(s): | Includes bibliography. | |
Held by: | Florida Atlantic University Libraries | |
Sublocation: | Digital Library | |
Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00012609 | |
Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
Host Institution: | FAU | |
Is Part of Series: | Florida Atlantic University Digital Library Collections. |