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CELLULAR SENESCENCE INCREASES IN THE ERCC1-/Δ MOUSE MODEL OF ACCELERATED AGING

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Date Issued:
2017
Abstract/Description:
Aging is the result of the progressive and intrinsic accumulation of detrimental changes in an organism over time. Understanding the molecular pathways that contribute to aging is critical for the development of therapeutic agents to treat age-related disorders. As an animal ages, it accumulates senescent cells, cells that are unable to grow or divide but remain metabolically active. They secrete Senescence Associated Secretory Phenotype (SASP) factors, which can disrupt tissue and cause age related diseases. The Ercc1-/Δ mice are an accelerated aging, progeriod model, and thus it was hypothesized that cellular senescence would increase in the Ercc1-/Δ mice. Through quantitative polymerase chain reaction (qPCR) analysis, the expression of senescence biomarkers p16INK4a, p21, IL-6 and TNF-α were measured to confirm that Ercc1-/Δ mice do experience an increase in cellular senescence. Additionally, we were able to determine that there are gender differences regarding the accumulation of senescent cells in various body parts.
Title: CELLULAR SENESCENCE INCREASES IN THE ERCC1-/Δ MOUSE MODEL OF ACCELERATED AGING.
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Name(s): Bukata, Christina , author
Wetterer, James K., Thesis advisor
Florida Atlantic University, Degree Grantor
Harriet L. Wilkes Honors College
Type of Resource: text
Genre: Thesis
Date Created: 2017
Date Issued: 2017
Publisher: Florida Atlantic University
Place of Publication: Jupiter, Florida
Physical Form: application/pdf
Extent: 38 p.
Language(s): English
Abstract/Description: Aging is the result of the progressive and intrinsic accumulation of detrimental changes in an organism over time. Understanding the molecular pathways that contribute to aging is critical for the development of therapeutic agents to treat age-related disorders. As an animal ages, it accumulates senescent cells, cells that are unable to grow or divide but remain metabolically active. They secrete Senescence Associated Secretory Phenotype (SASP) factors, which can disrupt tissue and cause age related diseases. The Ercc1-/Δ mice are an accelerated aging, progeriod model, and thus it was hypothesized that cellular senescence would increase in the Ercc1-/Δ mice. Through quantitative polymerase chain reaction (qPCR) analysis, the expression of senescence biomarkers p16INK4a, p21, IL-6 and TNF-α were measured to confirm that Ercc1-/Δ mice do experience an increase in cellular senescence. Additionally, we were able to determine that there are gender differences regarding the accumulation of senescent cells in various body parts.
Identifier: FA00012609 (IID)
Degree granted: Thesis (B.A.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2017.
Collection: FAU Honors Theses Digital Collection
Note(s): Includes bibliography.
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00012609
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.

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