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Development of a Biosensor to Detect Landfill Odors Using Human Odorant Binding Protein

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Date Issued:
2017
Summary:
Landfills receive odor complaints from local residents potentially resulting in legal ramifications and decreasing quality of life of the neighboring communities. However, analytical technologies to objectively measure odors in the field that will reflect human odor perceptions at a reasonable price do not currently exist. Hydrogen sulfide (H2S) is detectable by humans at low odor thresholds and may be one of the main nuisance odors emanating from landfills. For the first time, hOBPIIa complexed with 1- AMA has been used to detect H2S concentrations by spectrofluorometry. The optimal ratio of hOPBIIa-to-1-AMA was determined to be approximately 1:1. A strong linear regression model was generated to predict H2S concentrations from peak fluorescent emission intensity measurements within a range of 0-8.3 g of H2S. A novel experimental exposure chamber prototype was developed that has the potential to be incorporated into a portable sensor because it is compact.
Title: Development of a Biosensor to Detect Landfill Odors Using Human Odorant Binding Protein.
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Name(s): Roblyer, Julia Gia, author
Meeroff, Daniel E., Thesis advisor
Florida Atlantic University, Degree grantor
College of Engineering and Computer Science
Department of Civil, Environmental and Geomatics Engineering
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Date Created: 2017
Date Issued: 2017
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 117 p.
Language(s): English
Summary: Landfills receive odor complaints from local residents potentially resulting in legal ramifications and decreasing quality of life of the neighboring communities. However, analytical technologies to objectively measure odors in the field that will reflect human odor perceptions at a reasonable price do not currently exist. Hydrogen sulfide (H2S) is detectable by humans at low odor thresholds and may be one of the main nuisance odors emanating from landfills. For the first time, hOBPIIa complexed with 1- AMA has been used to detect H2S concentrations by spectrofluorometry. The optimal ratio of hOPBIIa-to-1-AMA was determined to be approximately 1:1. A strong linear regression model was generated to predict H2S concentrations from peak fluorescent emission intensity measurements within a range of 0-8.3 g of H2S. A novel experimental exposure chamber prototype was developed that has the potential to be incorporated into a portable sensor because it is compact.
Identifier: FA00005939 (IID)
Degree granted: Thesis (M.S.)--Florida Atlantic University, 2017.
Collection: FAU Electronic Theses and Dissertations Collection
Note(s): Includes bibliography.
Subject(s): Dissertations, Academic -- Florida Atlantic University
Landfills.
Biosensors.
Odor sensors.
Odor control.
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00005939
Use and Reproduction: Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.