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Pyroelectric Crystal Generated Very Low Dose X-rays Enhanced the Phytotherapeutic Effects of Beta-lapachone in Hormone Dependent Prostate Cancer Cells In Vitro
| Title: | Pyroelectric Crystal Generated Very Low Dose X-rays Enhanced the Phytotherapeutic Effects of Beta-lapachone in Hormone Dependent Prostate Cancer Cells In Vitro. |
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| Name(s): |
Oseni, Saheed Oluwasina Kumi-Diaka, James Jebelli, Joubin Graduate College Goldsmith, Harris Kaldas, George Branly, Rolando |
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| Type of Resource: | text | |
| Genre: | Poster | |
| Date Created: | 2015 | |
| Date Issued: | 2015 | |
| Publisher: | Florida Atlantic University | |
| Place of Publication: | Boca Raton, Fla. | |
| Physical Form: | application/pdf | |
| Extent: | 1 p. | |
| Language(s): | English | |
| Abstract/Description: | In 2015, an estimated 220, 800 new cases and 27, 540 deaths are expected to occur due to prostate cancer in US men, thus adding to the economic burden of the over 2.6 million men currently battling the disease. Plethora of studies have demonstrated the phytotherapeutic potentials of beta-lapachone, a phytochemical compound derived from the bark of the lapacho tree, native to South America. Betalapachone (β-lap) has been shown to exhibit its anti-cancer effects majorly by the futile cycling between the oxidized and the two electron reduction of β-lap mediated by NAD(P)H:quinone oxidoreductase (NQO1) resulting in the generation of reactive oxygen species (ROS) using NADH or NAD(P) as electron sources. β-lap is known to selectively kill human cancer cells, since NQO1 is expressed more abundantly in numerous human solid tumors than in the adjacent normal tissues; NQO1 has been shown to be exceptionally under expressed in hormone dependent prostate cancer cells (LNCaP) compared to the hormone independent prostate cancer cells (PC3). This study was aimed to investigate the enhancing effects of very low dose radiation (VLDR (20mGy)) derived from a pyroelectric crystal generator on the phytotherapeutic activity of beta-lapachone in LNCaP cell line in vitro accessed by MTT and Trypan blue assay. Treatment-induced intracellular levels of ROS were also assessed using Nitro blue tetrazolium assay. NQO1 activities in LNCaP cells were also investigated following treatment with VLDR and/or β-lap using Dicoumarol (NQO1 inhibitor). Results indicate that LNCaP cells respond significantly to combined treatments compared to single treatments. | |
| Identifier: | FA00005905 (IID) | |
| Collection: | FAU Student Research Digital Collection | |
| Note(s): | The Sixth Annual Graduate Research Day was organized by Florida Atlantic University’s Graduate Student Association. Graduate students from FAU Colleges present abstracts of original research and posters in a competition for monetary prizes, awards, and recognition. | |
| Held by: | Florida Atlantic University Libraries | |
| Sublocation: | Digital Library | |
| Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00005905 | |
| Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
| Host Institution: | FAU | |
| Is Part of Series: | Florida Atlantic University Digital Library Collections. |
