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Repairing spherical aberration during in vivo two-photon imaging

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Date Issued:
2015
Abstract/Description:
In vivo two photon microscopy generally requires a cranial window implant to aid in stabilization of the brain. These windows introduce aberration into the optical excitation, due to the use of glass coverslips and other media with different refractive index than that for which the microscope objective is designed. These mismatches introduce spherical aberration which can have dramatic effects on the axial pointspread function psf. We have developed a framework for computational simulations of typically used configurations including up to five media interfaces, as a means to understand the impact of spherical aberration, and to search for experimental solutions to correct for it. Our simulations suggest that spherical aberration even from a single glass coverslip ~170 microns can introduce a substantial loss of z-axis resolution, and blurring of signals from neighboring neural elements. We propose the use of opposite refractive index mismatch to correct for spherical aberration. Our simulations suggest that an appropriate magnitude of opposite refractive index can almost fully recover the ideal psf, and our experimental tests support these simulations.
Title: Repairing spherical aberration during in vivo two-photon imaging.
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Name(s): Estrada, Gerardo
Schummers, James
Graduate College
Type of Resource: text
Genre: Poster
Date Created: 2015
Date Issued: 2015
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 1 p.
Language(s): English
Abstract/Description: In vivo two photon microscopy generally requires a cranial window implant to aid in stabilization of the brain. These windows introduce aberration into the optical excitation, due to the use of glass coverslips and other media with different refractive index than that for which the microscope objective is designed. These mismatches introduce spherical aberration which can have dramatic effects on the axial pointspread function psf. We have developed a framework for computational simulations of typically used configurations including up to five media interfaces, as a means to understand the impact of spherical aberration, and to search for experimental solutions to correct for it. Our simulations suggest that spherical aberration even from a single glass coverslip ~170 microns can introduce a substantial loss of z-axis resolution, and blurring of signals from neighboring neural elements. We propose the use of opposite refractive index mismatch to correct for spherical aberration. Our simulations suggest that an appropriate magnitude of opposite refractive index can almost fully recover the ideal psf, and our experimental tests support these simulations.
Identifier: FA00005875 (IID)
Collection: FAU Student Research Digital Collection
Note(s): The Sixth Annual Graduate Research Day was organized by Florida Atlantic University’s Graduate Student Association. Graduate students from FAU Colleges present abstracts of original research and posters in a competition for monetary prizes, awards, and recognition.
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00005875
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.