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Unlocking the Phytotherapeutic Synergism between Beta-lapachone and Soybean-derived Genistein in Human Prostate cancer cells

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Date Issued:
2014
Summary:
Prostate cancer after many years is still the second most common cancer in American men with about 233,000 new cases and 29,480 deaths estimated to be occurring in 2014. Despite the wide spectra of reports demonstrating the anti-cancer phytotherapeutic potentials of beta-lapachone and soybean-derived genistein in various tumors, little emphasis had been placed on their synergistic effects in androgen-independent PC3 and androgen-dependent LNCaP prostate cancer cell lines. In this study, we aim to characterize the combined effects of genistein and b-lapachone on the phyto/chemosensitivity of LNCaP and PC3 human prostate cancer cells in-vitro, using MTT assay and LDH assay to study treatment-induced growth inhibition and cytotoxicity. Annexin-V-FITC and PI-TUNEL assays were also used to determine the potential treatment-induced apoptosis and/or necrosis. Our results revealed that both PC3 and LNCaP are phytosensitive to both single and combined treatments, though time-and dose-dependent. We observed that our treatments induced dual death pathways-apoptosis and necrosis-in both cell types and also observed that growth inhibition in both correlated positively with cell death in which, b-lapachone and genistein induced cell cycle arrest at the G1 and/or S phase and G2–M checkpoints respectively. Invariably, our results indicate that combination treatments with b-lapachone and genistein are more potent in killing both PC3 and LNCaP cancer cells than treatment with either genistein or b-lapachone alone. Our current results are therefore in agreement with the hypothesis that drugcombinations that target cell cycles at different critical checkpoints are more effective in causing cell death.
Title: Unlocking the Phytotherapeutic Synergism between Beta-lapachone and Soybean-derived Genistein in Human Prostate cancer cells.
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Name(s): Oseni, Saheed Oluwasina
Sandoval-Bernal, Bibiana
Kumi-Diaka, James
Graduate College
Type of Resource: text
Genre: Abstract
Date Created: 2014
Date Issued: 2014
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 1 p.
Language(s): English
Summary: Prostate cancer after many years is still the second most common cancer in American men with about 233,000 new cases and 29,480 deaths estimated to be occurring in 2014. Despite the wide spectra of reports demonstrating the anti-cancer phytotherapeutic potentials of beta-lapachone and soybean-derived genistein in various tumors, little emphasis had been placed on their synergistic effects in androgen-independent PC3 and androgen-dependent LNCaP prostate cancer cell lines. In this study, we aim to characterize the combined effects of genistein and b-lapachone on the phyto/chemosensitivity of LNCaP and PC3 human prostate cancer cells in-vitro, using MTT assay and LDH assay to study treatment-induced growth inhibition and cytotoxicity. Annexin-V-FITC and PI-TUNEL assays were also used to determine the potential treatment-induced apoptosis and/or necrosis. Our results revealed that both PC3 and LNCaP are phytosensitive to both single and combined treatments, though time-and dose-dependent. We observed that our treatments induced dual death pathways-apoptosis and necrosis-in both cell types and also observed that growth inhibition in both correlated positively with cell death in which, b-lapachone and genistein induced cell cycle arrest at the G1 and/or S phase and G2–M checkpoints respectively. Invariably, our results indicate that combination treatments with b-lapachone and genistein are more potent in killing both PC3 and LNCaP cancer cells than treatment with either genistein or b-lapachone alone. Our current results are therefore in agreement with the hypothesis that drugcombinations that target cell cycles at different critical checkpoints are more effective in causing cell death.
Identifier: FA00005845 (IID)
Collection: FAU Student Research Digital Collection
Note(s): The Fifth Annual Graduate Research Day was organized by Florida Atlantic University’s Graduate Student Association. Graduate students from FAU Colleges present abstracts of original research and posters in a competition for monetary prizes, awards, and recognition
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Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00005845
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Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.