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Effects of cGMP-dependent protein kinase signaling in Drosophila S2 cells subjected to anoxia and oxidative stress
- Date Issued:
- 2014
- Abstract/Description:
- Ischemic stroke is one of the leading causes of human death worldwide. It occurs due to high susceptibility of neurons to anoxia and reoxygenation. Unlike mammals, invertebrate Drosophila melanogaster withstands low oxygen levels without showing pathology. In the present study, Drosophila Schneider (S2) cells were employed to investigate the role of the cGMP-dependent Protein Kinase (PKG) signaling pathway in Drosophila anoxia-tolerance in vitro. This was accomplished by subjecting cells to chemical anoxia and oxidative stress concurrently with treatments by pharmacological agents affecting specific targets of the PKG pathway and assessing cytotoxicity. In this study, activation of the PKG pathway protected S2 cells from the aforementioned stresses. The results of this study may lead to a better understanding of fruit fly’s innate anoxia-tolerance strategies. Consequently, this knowledge may be used to identify potential therapeutic targets to prevent detrimental neurological effects of an ischemic stroke in humans.
Title: | Effects of cGMP-dependent protein kinase signaling in Drosophila S2 cells subjected to anoxia and oxidative stress. |
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Name(s): |
Makhnyeva, Olena Milton, Sarah L. Dawson-Scully, Ken Graduate College |
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Type of Resource: | text | |
Genre: | Abstract | |
Date Created: | 2014 | |
Date Issued: | 2014 | |
Publisher: | Florida Atlantic University | |
Place of Publication: | Boca Raton, Fla. | |
Physical Form: | application/pdf | |
Extent: | 1 p. | |
Language(s): | English | |
Abstract/Description: | Ischemic stroke is one of the leading causes of human death worldwide. It occurs due to high susceptibility of neurons to anoxia and reoxygenation. Unlike mammals, invertebrate Drosophila melanogaster withstands low oxygen levels without showing pathology. In the present study, Drosophila Schneider (S2) cells were employed to investigate the role of the cGMP-dependent Protein Kinase (PKG) signaling pathway in Drosophila anoxia-tolerance in vitro. This was accomplished by subjecting cells to chemical anoxia and oxidative stress concurrently with treatments by pharmacological agents affecting specific targets of the PKG pathway and assessing cytotoxicity. In this study, activation of the PKG pathway protected S2 cells from the aforementioned stresses. The results of this study may lead to a better understanding of fruit fly’s innate anoxia-tolerance strategies. Consequently, this knowledge may be used to identify potential therapeutic targets to prevent detrimental neurological effects of an ischemic stroke in humans. | |
Identifier: | FA00005837 (IID) | |
Collection: | FAU Student Research Digital Collection | |
Note(s): | The Fifth Annual Graduate Research Day was organized by Florida Atlantic University’s Graduate Student Association. Graduate students from FAU Colleges present abstracts of original research and posters in a competition for monetary prizes, awards, and recognition | |
Held by: | Florida Atlantic University Libraries | |
Sublocation: | Digital Library | |
Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00005837 | |
Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
Host Institution: | FAU | |
Is Part of Series: | Florida Atlantic University Digital Library Collections. |