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Novel function of PTP69D in giant fiber synapse formation

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Date Issued:
2014
Summary:
PTP69D is a receptor protein tyrosine phosphatase RPTP with two intracellular catalytic domains Cat1 and Cat2, which has been shown to play a role in axon outgrowth and guidance of embryonic motoneurons as well as targeting of photoreceptor neurons in the visual system of Drosophila melanogaster. Here, we characterized the developmental role of PTP69D in the giant fiber GF neurons; two interneurons in the central nervous system CNS that control the escape response of the fly. In addition to guidance and targeting functions, our studies reveal an additional role for PTP69D in synaptic terminal growth in the CNS. We found that inhibition of phosphatase activity in catalytic domain Cat1 proximal to the transmembrane domain did not affect axon guidance or targeting but resulted in stunted terminal growth of the GFs. Cell autonomous rescue and knockdown experiments demonstrated a function for PTP69D in the GFs, but not its postsynaptic target neurons. In addition, complementation studies and structurefunction analyses revealed that for GF terminal growth Cat1 function of PTP69D requires the Immunoglobulin and the Cat2 domain, but not the Fibronectin III and the Membrane Proximal Region domains. In contrast, the Fibronectin III, but not the Immunoglobulin domains, were previously shown to be essential for axon targeting of photoreceptor neurons. Thus, our studies uncover a novel role for PTP69D in synaptic terminal growth in the CNS that is mechanistically distinct from its function during earlier developmental process.
Title: Novel function of PTP69D in giant fiber synapse formation.
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Name(s): Lee, LaTasha Hoskins
Godenschwege, Tanja A.
Graduate College
Type of Resource: text
Genre: Abstract
Date Created: 2014
Date Issued: 2014
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 1 p.
Language(s): English
Summary: PTP69D is a receptor protein tyrosine phosphatase RPTP with two intracellular catalytic domains Cat1 and Cat2, which has been shown to play a role in axon outgrowth and guidance of embryonic motoneurons as well as targeting of photoreceptor neurons in the visual system of Drosophila melanogaster. Here, we characterized the developmental role of PTP69D in the giant fiber GF neurons; two interneurons in the central nervous system CNS that control the escape response of the fly. In addition to guidance and targeting functions, our studies reveal an additional role for PTP69D in synaptic terminal growth in the CNS. We found that inhibition of phosphatase activity in catalytic domain Cat1 proximal to the transmembrane domain did not affect axon guidance or targeting but resulted in stunted terminal growth of the GFs. Cell autonomous rescue and knockdown experiments demonstrated a function for PTP69D in the GFs, but not its postsynaptic target neurons. In addition, complementation studies and structurefunction analyses revealed that for GF terminal growth Cat1 function of PTP69D requires the Immunoglobulin and the Cat2 domain, but not the Fibronectin III and the Membrane Proximal Region domains. In contrast, the Fibronectin III, but not the Immunoglobulin domains, were previously shown to be essential for axon targeting of photoreceptor neurons. Thus, our studies uncover a novel role for PTP69D in synaptic terminal growth in the CNS that is mechanistically distinct from its function during earlier developmental process.
Identifier: FA00005831 (IID)
Collection: FAU Student Research Digital Collection
Note(s): The Fifth Annual Graduate Research Day was organized by Florida Atlantic University’s Graduate Student Association. Graduate students from FAU Colleges present abstracts of original research and posters in a competition for monetary prizes, awards, and recognition
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00005831
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Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.