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Selective Activation of the SK1 Subtype of Small Conductance Ca2+-Activated K+Channels by 4-(2-Methoxyphenylcarbamoyloxymethyl)-piperidine-l-carboxylic Acid tert-Butyl Ester(GW542573X) in C57BL/6J Mice lmpairs Hippocampal-Dependent Memory.
| Title: | Selective Activation of the SK1 Subtype of Small Conductance Ca2+-Activated K+Channels by 4-(2-Methoxyphenylcarbamoyloxymethyl)-piperidine-l-carboxylic Acid tert-Butyl Ester(GW542573X) in C57BL/6J Mice lmpairs Hippocampal-Dependent Memory. |
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| Name(s): |
Kuchera, Claire Rice Stackman, Robert W. Graduate College |
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| Type of Resource: | text | |
| Genre: | Abstract | |
| Date Created: | 2014 | |
| Date Issued: | 2014 | |
| Publisher: | Florida Atlantic University | |
| Place of Publication: | Boca Raton, Fla. | |
| Physical Form: | application/pdf | |
| Extent: | 1 p. | |
| Language(s): | English | |
| Abstract/Description: | Small conductance Ca2+ -activated K+ (SK) channels have physiological roles in learning and memory, intrinsic excitability, synaptic transmission and plasticity, and addiction. While SK2 and SK3 channels have been studied, the role of SK1 has not yet been determined due to the prior lack of gene-specific antibodies and agonists. SK1 are robustly expressed in the CA1 pyramidal neurons of the hippocampus and modulate their excitability by affecting afterhyperpolarization. SK1 subunits are only sited in the plasma membrane when co-expressed with SK2 or SK3. Co-expressed and co-assembled SK1, SK2, and SK3 subunits form functional apamin-sensitive channels. SK1 are not apamin selective, suggesting the overriding hypothesis that SK1 is a subunit of heteromeric SK channels that bind specific interacting proteins. We examined the effect of a new SK1 selective activator, GW542573X, on hippocampal dependent object memory in male C57BL/6J mice. The results showed that activating SK1 channels by systemic injection of the SK1 agonist GW542573X before the sample session, led to impaired object memory in mice 24 h later. Mice treated with GW542573X acquired the sample object exploration criterion in a similar latency as the vehicle-treated mice.GW542573X treated mice exhibited significantly less preference for exploring the novel object during the test session compared to the vehicle-treated mice. These results suggest that the SK1 activator disrupted the encoding of object memory without affecting the motivation to explore objects. This supports a role for SK1 in the modulation of hippocampal synaptic plasticity and hippocampal-dependent memory. | |
| Identifier: | FA00005830 (IID) | |
| Collection: | FAU Student Research Digital Collection | |
| Note(s): | The Fifth Annual Graduate Research Day was organized by Florida Atlantic University’s Graduate Student Association. Graduate students from FAU Colleges present abstracts of original research and posters in a competition for monetary prizes, awards, and recognition | |
| Held by: | Florida Atlantic University Libraries | |
| Sublocation: | Digital Library | |
| Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00005830 | |
| Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
| Host Institution: | FAU | |
| Is Part of Series: | Florida Atlantic University Digital Library Collections. |
