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Object-specific activity recorded from C57BL/6J mouse hippocampal CA1 neurons

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Date Issued:
2014
Summary:
The rodent hippocampus is an essential neural substrate for spatial memory. This functional capacity is considered to rely upon a cognitive map that represents the location where relevant non-spatial items or objects are encountered and where specific events occur within a contextual or spatial reference frame. Place cell activity recorded from CA1 pyramidal neurons of the dorsal hippocampus of freely moving rodents is influenced by distal and proximal cues or items within an environment, and increases when objects are placed into a familiar arena. Recently, the CA1 region of the rodent dorsal hippocampus was shown to play a vital role in object-in-context memory, and object memory independent of context; findings consistent with the cognitive map view. Here, we tested the influence of 3D objects on the spatial firing properties of CA1 neurons, since object-specific neuronal activity has not yet been fully established in mouse hippocampus. In vivo extracellular recordings from intermediate dorsal CA1 yielded simultaneous recordings of place cells and a pyramidal neuron demonstrating object-specific activity over two consecutive sessions with objects present. Higher frequency object-specific activity was recorded from the same mouse again 3 weeks later during a comparable task. Object-specific activity was observed only when the mouse explored objects in the arena, and was independent of spatial location or object identity. Recordings from more distal region of dorsal CA1, which receives input from proximal CA3, yielded two additional neurons that demonstrated comparable object-related activity. These results further support the involvement of the rodent hippocampus in non-spatial object memory.
Title: Object-specific activity recorded from C57BL/6J mouse hippocampal CA1 neurons.
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Name(s): Asgeirsdottir, Herborg Nanna
Stackman, Robert W.
Graduate College
Type of Resource: text
Genre: Abstract
Date Created: 2014
Date Issued: 2014
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 1 p.
Language(s): English
Summary: The rodent hippocampus is an essential neural substrate for spatial memory. This functional capacity is considered to rely upon a cognitive map that represents the location where relevant non-spatial items or objects are encountered and where specific events occur within a contextual or spatial reference frame. Place cell activity recorded from CA1 pyramidal neurons of the dorsal hippocampus of freely moving rodents is influenced by distal and proximal cues or items within an environment, and increases when objects are placed into a familiar arena. Recently, the CA1 region of the rodent dorsal hippocampus was shown to play a vital role in object-in-context memory, and object memory independent of context; findings consistent with the cognitive map view. Here, we tested the influence of 3D objects on the spatial firing properties of CA1 neurons, since object-specific neuronal activity has not yet been fully established in mouse hippocampus. In vivo extracellular recordings from intermediate dorsal CA1 yielded simultaneous recordings of place cells and a pyramidal neuron demonstrating object-specific activity over two consecutive sessions with objects present. Higher frequency object-specific activity was recorded from the same mouse again 3 weeks later during a comparable task. Object-specific activity was observed only when the mouse explored objects in the arena, and was independent of spatial location or object identity. Recordings from more distal region of dorsal CA1, which receives input from proximal CA3, yielded two additional neurons that demonstrated comparable object-related activity. These results further support the involvement of the rodent hippocampus in non-spatial object memory.
Identifier: FA00005801 (IID)
Collection: FAU Student Research Digital Collection
Note(s): The Fifth Annual Graduate Research Day was organized by Florida Atlantic University’s Graduate Student Association. Graduate students from FAU Colleges present abstracts of original research and posters in a competition for monetary prizes, awards, and recognition
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00005801
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Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.