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Enhanced CpG Activated Macrophage Killing of 3-Bromopyruvate Pre-treated 4T1 Breast Cancer Cells

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Date Issued:
2017
Summary:
A common feature of breast cancer cells is the evasion of singular treatments by using the Warburg Effect, a process of metabolic ATP production through rapid anaerobic glycolysis. Cancer research has transitioned to an investigation of combination therapies to combat cancer. In our study, we seek to metabolically inhibit cancer cells before application of immunogenic killing. The Warburg Effect was targeted with 3-Bromopyruvate (3-BP), which blocks Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) production. Treatment with 3-BP yielded up to 86.5% cancer cell death. Glycolytic inhibition renders cancer cells metabolically stressed, which may enable an effective immune response. Our hypothesis was that CpG activated macrophage will possess tumoricidal potential to target metabolically stressed cancer cells. Macrophages and CpG cultivation alone yielded a significant immune response. We sought to find a synergistic effect of 3-BP induced killing susceptibility with CpG activated macrophages may lead to an effective method of combination therapy.
Title: Enhanced CpG Activated Macrophage Killing of 3-Bromopyruvate Pre-treated 4T1 Breast Cancer Cells.
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Name(s): Rumicha, Dawit
Liddle, Genevieve M.
Hartmann, James X.
Office of Undergraduate Research and Inquiry
Type of Resource: text
Genre: Poster
Date Created: 2017
Date Issued: 2017
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Florida
Physical Form: application/pdf
Extent: 1 p.
Language(s): English
Summary: A common feature of breast cancer cells is the evasion of singular treatments by using the Warburg Effect, a process of metabolic ATP production through rapid anaerobic glycolysis. Cancer research has transitioned to an investigation of combination therapies to combat cancer. In our study, we seek to metabolically inhibit cancer cells before application of immunogenic killing. The Warburg Effect was targeted with 3-Bromopyruvate (3-BP), which blocks Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) production. Treatment with 3-BP yielded up to 86.5% cancer cell death. Glycolytic inhibition renders cancer cells metabolically stressed, which may enable an effective immune response. Our hypothesis was that CpG activated macrophage will possess tumoricidal potential to target metabolically stressed cancer cells. Macrophages and CpG cultivation alone yielded a significant immune response. We sought to find a synergistic effect of 3-BP induced killing susceptibility with CpG activated macrophages may lead to an effective method of combination therapy.
Identifier: FA00005634 (IID)
Subject(s): College students --Research --United States.
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00005634
Restrictions on Access: Author retains rights.
Host Institution: FAU